Experiment: D-Cycloserine 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_1744 and Sama_1745 are separated by 73 nucleotides Sama_1745 and Sama_1746 are separated by 208 nucleotides Sama_1746 and pssA are separated by 11 nucleotides
Sama_1744: Sama_1744 - Serine-type D-Ala-D-Ala carboxypeptidase (RefSeq), at 2,134,055 to 2,135,719
_1744
Sama_1745: Sama_1745 - hypothetical protein (RefSeq), at 2,135,793 to 2,135,978
_1745
Sama_1746: Sama_1746 - hypothetical protein (RefSeq), at 2,136,187 to 2,136,513
_1746
Sama_1747: pssA - phosphatidylserine synthase (RefSeq), at 2,136,525 to 2,137,838
pssA
Position (kb)
2136
2137 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2135.221 kb on + strand, within Sama_1744 at 2135.221 kb on + strand, within Sama_1744 at 2135.221 kb on + strand, within Sama_1744 at 2135.323 kb on + strand, within Sama_1744 at 2135.324 kb on - strand, within Sama_1744 at 2135.324 kb on - strand, within Sama_1744 at 2135.411 kb on - strand, within Sama_1744 at 2135.411 kb on - strand, within Sama_1744 at 2135.654 kb on + strand at 2135.661 kb on - strand at 2135.708 kb on + strand at 2135.709 kb on - strand at 2135.742 kb on - strand at 2135.834 kb on - strand, within Sama_1745 at 2135.850 kb on - strand, within Sama_1745 at 2135.850 kb on - strand, within Sama_1745 at 2135.850 kb on - strand, within Sama_1745 at 2135.944 kb on - strand, within Sama_1745 at 2135.989 kb on + strand at 2135.996 kb on + strand at 2135.997 kb on - strand at 2135.997 kb on - strand at 2136.015 kb on - strand at 2136.016 kb on + strand at 2136.016 kb on + strand at 2136.031 kb on - strand at 2136.039 kb on - strand at 2136.048 kb on - strand at 2136.054 kb on + strand at 2136.074 kb on + strand at 2136.075 kb on - strand at 2136.075 kb on - strand at 2136.114 kb on + strand at 2136.292 kb on + strand, within Sama_1746 at 2136.292 kb on + strand, within Sama_1746 at 2136.465 kb on + strand, within Sama_1746 at 2136.490 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.03125 mg/ml remove 2,135,221 + Sama_1744 0.70 +1.3 2,135,221 + Sama_1744 0.70 -1.4 2,135,221 + Sama_1744 0.70 -0.1 2,135,323 + Sama_1744 0.76 +1.0 2,135,324 - Sama_1744 0.76 +0.9 2,135,324 - Sama_1744 0.76 +1.7 2,135,411 - Sama_1744 0.81 +0.3 2,135,411 - Sama_1744 0.81 +1.0 2,135,654 + +0.6 2,135,661 - +0.4 2,135,708 + -0.1 2,135,709 - +0.2 2,135,742 - +1.2 2,135,834 - Sama_1745 0.22 +0.0 2,135,850 - Sama_1745 0.31 -0.0 2,135,850 - Sama_1745 0.31 +0.9 2,135,850 - Sama_1745 0.31 -0.9 2,135,944 - Sama_1745 0.81 +1.5 2,135,989 + -1.3 2,135,996 + +1.3 2,135,997 - -1.5 2,135,997 - -0.7 2,136,015 - +0.3 2,136,016 + -0.5 2,136,016 + +0.6 2,136,031 - -1.1 2,136,039 - -1.7 2,136,048 - -0.7 2,136,054 + -0.6 2,136,074 + +1.8 2,136,075 - -1.6 2,136,075 - +1.3 2,136,114 + -0.0 2,136,292 + Sama_1746 0.32 +0.3 2,136,292 + Sama_1746 0.32 -0.7 2,136,465 + Sama_1746 0.85 -1.5 2,136,490 + -2.7
Or see this region's nucleotide sequence