Experiment: D-Cycloserine 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpoZ and gmk are separated by 77 nucleotides gmk and Sama_0267 are separated by 187 nucleotides Sama_0267 and Sama_0268 are separated by 85 nucleotides Sama_0268 and Sama_0269 overlap by 8 nucleotides
Sama_0265: rpoZ - DNA-directed RNA polymerase subunit omega (RefSeq), at 304,957 to 305,235
rpoZ
Sama_0266: gmk - guanylate kinase (RefSeq), at 305,313 to 305,936
gmk
Sama_0267: Sama_0267 - hypothetical protein (RefSeq), at 306,124 to 307,608
_0267
Sama_0268: Sama_0268 - hypothetical protein (RefSeq), at 307,694 to 308,560
_0268
Sama_0269: Sama_0269 - hypothetical protein (RefSeq), at 308,553 to 309,617
_0269
Position (kb) 306
307
308 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 305.218 kb on + strand at 305.219 kb on - strand at 305.219 kb on - strand at 305.219 kb on - strand at 305.225 kb on - strand at 305.238 kb on - strand at 305.238 kb on - strand at 305.263 kb on - strand at 305.263 kb on - strand at 305.301 kb on - strand at 305.301 kb on - strand at 305.305 kb on + strand at 305.305 kb on + strand at 305.306 kb on - strand at 305.306 kb on - strand at 305.306 kb on - strand at 305.333 kb on + strand at 305.334 kb on - strand at 305.339 kb on + strand at 305.339 kb on + strand at 305.339 kb on + strand at 305.339 kb on + strand at 305.340 kb on - strand at 305.340 kb on - strand at 305.373 kb on + strand at 306.001 kb on - strand at 306.001 kb on - strand at 306.013 kb on - strand at 306.013 kb on - strand at 306.015 kb on - strand at 306.015 kb on - strand at 306.081 kb on - strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.097 kb on + strand at 306.097 kb on + strand at 306.097 kb on + strand at 306.097 kb on + strand at 306.097 kb on + strand at 306.097 kb on + strand at 306.097 kb on + strand at 306.097 kb on + strand at 306.097 kb on + strand at 306.098 kb on - strand at 306.098 kb on - strand at 306.098 kb on - strand at 306.098 kb on - strand at 306.098 kb on - strand at 306.145 kb on - strand at 306.145 kb on - strand at 306.175 kb on - strand at 306.200 kb on - strand at 306.200 kb on - strand at 306.200 kb on - strand at 306.200 kb on - strand at 306.332 kb on - strand, within Sama_0267 at 306.372 kb on - strand, within Sama_0267 at 306.382 kb on - strand, within Sama_0267 at 306.382 kb on - strand, within Sama_0267 at 306.432 kb on + strand, within Sama_0267 at 306.432 kb on + strand, within Sama_0267 at 306.432 kb on + strand, within Sama_0267 at 306.432 kb on + strand, within Sama_0267 at 306.432 kb on + strand, within Sama_0267 at 306.432 kb on + strand, within Sama_0267 at 306.433 kb on - strand, within Sama_0267 at 306.478 kb on + strand, within Sama_0267 at 306.683 kb on + strand, within Sama_0267 at 306.730 kb on + strand, within Sama_0267 at 306.730 kb on + strand, within Sama_0267 at 306.936 kb on + strand, within Sama_0267 at 306.937 kb on - strand, within Sama_0267 at 306.987 kb on + strand, within Sama_0267 at 307.012 kb on + strand, within Sama_0267 at 307.012 kb on + strand, within Sama_0267 at 307.012 kb on + strand, within Sama_0267 at 307.012 kb on + strand, within Sama_0267 at 307.013 kb on - strand, within Sama_0267 at 307.102 kb on + strand, within Sama_0267 at 307.102 kb on + strand, within Sama_0267 at 307.102 kb on + strand, within Sama_0267 at 307.102 kb on + strand, within Sama_0267 at 307.102 kb on + strand, within Sama_0267 at 307.102 kb on + strand, within Sama_0267 at 307.102 kb on + strand, within Sama_0267 at 307.102 kb on + strand, within Sama_0267 at 307.103 kb on - strand, within Sama_0267 at 307.103 kb on - strand, within Sama_0267 at 307.113 kb on + strand, within Sama_0267 at 307.132 kb on + strand, within Sama_0267 at 307.132 kb on + strand, within Sama_0267 at 307.132 kb on + strand, within Sama_0267 at 307.132 kb on + strand, within Sama_0267 at 307.133 kb on - strand, within Sama_0267 at 307.136 kb on + strand, within Sama_0267 at 307.185 kb on + strand, within Sama_0267 at 307.214 kb on + strand, within Sama_0267 at 307.214 kb on + strand, within Sama_0267 at 307.214 kb on + strand, within Sama_0267 at 307.214 kb on + strand, within Sama_0267 at 307.215 kb on - strand, within Sama_0267 at 307.215 kb on - strand, within Sama_0267 at 307.347 kb on + strand, within Sama_0267 at 307.347 kb on + strand, within Sama_0267 at 307.347 kb on + strand, within Sama_0267 at 307.347 kb on + strand, within Sama_0267 at 307.348 kb on - strand, within Sama_0267 at 307.348 kb on - strand, within Sama_0267 at 307.348 kb on - strand, within Sama_0267 at 307.348 kb on - strand, within Sama_0267 at 307.348 kb on - strand, within Sama_0267 at 307.348 kb on - strand, within Sama_0267 at 307.348 kb on - strand, within Sama_0267 at 307.402 kb on - strand, within Sama_0267 at 307.488 kb on + strand at 307.488 kb on + strand at 307.489 kb on - strand at 307.489 kb on - strand at 307.626 kb on + strand at 307.641 kb on + strand at 307.641 kb on + strand at 307.641 kb on + strand at 307.641 kb on + strand at 307.641 kb on + strand at 307.641 kb on + strand at 307.641 kb on + strand at 307.641 kb on + strand at 307.641 kb on + strand at 307.641 kb on + strand at 307.641 kb on + strand at 307.641 kb on + strand at 307.641 kb on + strand at 307.641 kb on + strand at 307.642 kb on - strand at 307.642 kb on - strand at 307.642 kb on - strand at 307.642 kb on - strand at 307.642 kb on - strand at 307.642 kb on - strand at 307.655 kb on + strand at 307.655 kb on + strand at 307.655 kb on + strand at 307.655 kb on + strand at 307.656 kb on - strand at 307.685 kb on + strand at 307.685 kb on + strand at 307.685 kb on + strand at 307.685 kb on + strand at 307.686 kb on - strand at 307.686 kb on - strand at 307.686 kb on - strand at 307.686 kb on - strand at 307.686 kb on - strand at 307.692 kb on + strand at 307.692 kb on + strand at 307.692 kb on + strand at 307.692 kb on + strand at 307.693 kb on - strand at 307.693 kb on - strand at 307.693 kb on - strand at 307.693 kb on - strand at 307.693 kb on - strand at 307.693 kb on - strand at 307.716 kb on + strand at 307.716 kb on + strand at 307.717 kb on - strand at 307.717 kb on - strand at 307.717 kb on - strand at 307.717 kb on - strand at 307.736 kb on - strand at 307.736 kb on - strand at 307.753 kb on + strand at 307.753 kb on + strand at 307.754 kb on - strand at 307.843 kb on - strand, within Sama_0268 at 307.851 kb on + strand, within Sama_0268 at 307.852 kb on - strand, within Sama_0268 at 308.078 kb on + strand, within Sama_0268 at 308.078 kb on + strand, within Sama_0268 at 308.116 kb on + strand, within Sama_0268 at 308.145 kb on - strand, within Sama_0268 at 308.181 kb on + strand, within Sama_0268 at 308.193 kb on + strand, within Sama_0268 at 308.193 kb on + strand, within Sama_0268 at 308.193 kb on + strand, within Sama_0268 at 308.193 kb on + strand, within Sama_0268 at 308.193 kb on + strand, within Sama_0268 at 308.193 kb on + strand, within Sama_0268 at 308.194 kb on - strand, within Sama_0268 at 308.194 kb on - strand, within Sama_0268 at 308.194 kb on - strand, within Sama_0268 at 308.213 kb on + strand, within Sama_0268 at 308.214 kb on - strand, within Sama_0268 at 308.214 kb on - strand, within Sama_0268 at 308.234 kb on + strand, within Sama_0268 at 308.234 kb on + strand, within Sama_0268 at 308.235 kb on - strand, within Sama_0268 at 308.235 kb on - strand, within Sama_0268 at 308.235 kb on - strand, within Sama_0268 at 308.235 kb on - strand, within Sama_0268 at 308.235 kb on - strand, within Sama_0268 at 308.235 kb on - strand, within Sama_0268 at 308.244 kb on + strand, within Sama_0268 at 308.244 kb on + strand, within Sama_0268 at 308.244 kb on + strand, within Sama_0268 at 308.244 kb on + strand, within Sama_0268 at 308.244 kb on + strand, within Sama_0268 at 308.244 kb on + strand, within Sama_0268 at 308.244 kb on + strand, within Sama_0268 at 308.244 kb on + strand, within Sama_0268 at 308.245 kb on - strand, within Sama_0268 at 308.245 kb on - strand, within Sama_0268 at 308.245 kb on - strand, within Sama_0268 at 308.245 kb on - strand, within Sama_0268 at 308.245 kb on - strand, within Sama_0268 at 308.245 kb on - strand, within Sama_0268 at 308.245 kb on - strand, within Sama_0268 at 308.265 kb on + strand, within Sama_0268 at 308.265 kb on + strand, within Sama_0268 at 308.265 kb on + strand, within Sama_0268 at 308.266 kb on - strand, within Sama_0268 at 308.266 kb on - strand, within Sama_0268 at 308.279 kb on - strand, within Sama_0268 at 308.373 kb on + strand, within Sama_0268 at 308.374 kb on - strand, within Sama_0268 at 308.374 kb on - strand, within Sama_0268 at 308.374 kb on - strand, within Sama_0268 at 308.374 kb on - strand, within Sama_0268 at 308.376 kb on + strand, within Sama_0268 at 308.377 kb on - strand, within Sama_0268 at 308.417 kb on + strand, within Sama_0268 at 308.417 kb on + strand, within Sama_0268 at 308.417 kb on + strand, within Sama_0268 at 308.417 kb on + strand, within Sama_0268 at 308.417 kb on + strand, within Sama_0268 at 308.418 kb on - strand, within Sama_0268 at 308.418 kb on - strand, within Sama_0268 at 308.418 kb on - strand, within Sama_0268 at 308.418 kb on - strand, within Sama_0268 at 308.418 kb on - strand, within Sama_0268 at 308.501 kb on - strand at 308.501 kb on - strand at 308.504 kb on + strand at 308.504 kb on + strand at 308.504 kb on + strand at 308.504 kb on + strand at 308.504 kb on + strand at 308.504 kb on + strand at 308.505 kb on - strand at 308.505 kb on - strand at 308.505 kb on - strand at 308.527 kb on - strand at 308.527 kb on - strand at 308.547 kb on + strand at 308.547 kb on + strand at 308.547 kb on + strand at 308.547 kb on + strand at 308.547 kb on + strand at 308.547 kb on + strand at 308.548 kb on - strand at 308.589 kb on + strand at 308.589 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.03125 mg/ml remove 305,218 + +0.8 305,219 - +1.3 305,219 - +0.9 305,219 - +1.5 305,225 - +1.0 305,238 - +1.9 305,238 - +0.9 305,263 - +0.7 305,263 - +1.0 305,301 - +0.4 305,301 - +0.4 305,305 + -1.0 305,305 + -0.0 305,306 - +0.7 305,306 - -0.8 305,306 - +0.8 305,333 + +1.5 305,334 - +0.4 305,339 + -0.8 305,339 + -0.2 305,339 + -1.6 305,339 + -0.6 305,340 - -0.1 305,340 - +0.8 305,373 + -1.2 306,001 - +0.5 306,001 - +0.1 306,013 - +0.4 306,013 - -0.6 306,015 - -1.6 306,015 - -0.9 306,081 - -0.6 306,095 + +0.4 306,095 + +1.2 306,095 + -1.0 306,095 + +0.1 306,095 + +0.1 306,095 + -0.2 306,095 + +0.7 306,095 + +0.8 306,095 + -0.0 306,095 + +0.2 306,095 + -1.7 306,096 - -1.5 306,096 - -0.1 306,096 - -1.7 306,096 - -0.2 306,096 - +0.5 306,096 - +0.8 306,096 - -2.3 306,096 - +0.9 306,096 - +0.8 306,096 - -1.4 306,096 - +0.2 306,097 + -0.4 306,097 + +0.2 306,097 + -0.0 306,097 + -1.4 306,097 + -0.3 306,097 + -0.9 306,097 + +0.4 306,097 + -0.1 306,097 + +0.4 306,098 - +1.0 306,098 - -0.1 306,098 - -0.6 306,098 - -1.6 306,098 - +0.1 306,145 - +0.4 306,145 - +1.0 306,175 - +1.8 306,200 - +1.1 306,200 - +0.2 306,200 - -0.7 306,200 - -0.8 306,332 - Sama_0267 0.14 +0.1 306,372 - Sama_0267 0.17 -0.0 306,382 - Sama_0267 0.17 -2.3 306,382 - Sama_0267 0.17 -0.2 306,432 + Sama_0267 0.21 +0.6 306,432 + Sama_0267 0.21 -1.8 306,432 + Sama_0267 0.21 -2.0 306,432 + Sama_0267 0.21 -0.7 306,432 + Sama_0267 0.21 +0.1 306,432 + Sama_0267 0.21 -1.1 306,433 - Sama_0267 0.21 -0.4 306,478 + Sama_0267 0.24 -0.7 306,683 + Sama_0267 0.38 -0.7 306,730 + Sama_0267 0.41 -0.2 306,730 + Sama_0267 0.41 +0.2 306,936 + Sama_0267 0.55 -0.8 306,937 - Sama_0267 0.55 +0.2 306,987 + Sama_0267 0.58 -0.8 307,012 + Sama_0267 0.60 +0.4 307,012 + Sama_0267 0.60 +0.3 307,012 + Sama_0267 0.60 +0.1 307,012 + Sama_0267 0.60 +1.5 307,013 - Sama_0267 0.60 -2.4 307,102 + Sama_0267 0.66 -0.6 307,102 + Sama_0267 0.66 -1.4 307,102 + Sama_0267 0.66 -1.9 307,102 + Sama_0267 0.66 +0.3 307,102 + Sama_0267 0.66 -1.2 307,102 + Sama_0267 0.66 -0.9 307,102 + Sama_0267 0.66 -0.2 307,102 + Sama_0267 0.66 -1.0 307,103 - Sama_0267 0.66 +0.0 307,103 - Sama_0267 0.66 +1.2 307,113 + Sama_0267 0.67 +0.3 307,132 + Sama_0267 0.68 -1.5 307,132 + Sama_0267 0.68 -0.0 307,132 + Sama_0267 0.68 +1.8 307,132 + Sama_0267 0.68 +1.0 307,133 - Sama_0267 0.68 -0.7 307,136 + Sama_0267 0.68 -0.6 307,185 + Sama_0267 0.71 -0.7 307,214 + Sama_0267 0.73 -0.8 307,214 + Sama_0267 0.73 +0.5 307,214 + Sama_0267 0.73 +1.4 307,214 + Sama_0267 0.73 -0.6 307,215 - Sama_0267 0.73 -0.2 307,215 - Sama_0267 0.73 -0.2 307,347 + Sama_0267 0.82 +0.4 307,347 + Sama_0267 0.82 +0.1 307,347 + Sama_0267 0.82 -0.4 307,347 + Sama_0267 0.82 +0.2 307,348 - Sama_0267 0.82 -0.1 307,348 - Sama_0267 0.82 -1.2 307,348 - Sama_0267 0.82 -3.2 307,348 - Sama_0267 0.82 -0.2 307,348 - Sama_0267 0.82 -0.6 307,348 - Sama_0267 0.82 -0.0 307,348 - Sama_0267 0.82 -0.8 307,402 - Sama_0267 0.86 +0.6 307,488 + +0.8 307,488 + -0.4 307,489 - +0.1 307,489 - -0.6 307,626 + +1.1 307,641 + +0.4 307,641 + -0.6 307,641 + -0.5 307,641 + -0.1 307,641 + +0.4 307,641 + +0.7 307,641 + +0.7 307,641 + -0.2 307,641 + +0.5 307,641 + -0.2 307,641 + +0.1 307,641 + +0.4 307,641 + -0.3 307,641 + -0.4 307,642 - -1.3 307,642 - +1.8 307,642 - +1.4 307,642 - -1.1 307,642 - +0.1 307,642 - +0.7 307,655 + +0.1 307,655 + +0.2 307,655 + -0.0 307,655 + +0.0 307,656 - -0.7 307,685 + +0.3 307,685 + +0.1 307,685 + -0.6 307,685 + -0.5 307,686 - -1.1 307,686 - -1.0 307,686 - +0.5 307,686 - +0.4 307,686 - -1.5 307,692 + +1.5 307,692 + -0.6 307,692 + +0.1 307,692 + -0.1 307,693 - -1.6 307,693 - +0.0 307,693 - +1.0 307,693 - -1.0 307,693 - +0.7 307,693 - -0.6 307,716 + -2.1 307,716 + -0.2 307,717 - -1.0 307,717 - -0.9 307,717 - +0.4 307,717 - +0.6 307,736 - -2.2 307,736 - -0.7 307,753 + -1.8 307,753 + +0.8 307,754 - +0.4 307,843 - Sama_0268 0.17 +0.4 307,851 + Sama_0268 0.18 -0.2 307,852 - Sama_0268 0.18 -0.3 308,078 + Sama_0268 0.44 -0.4 308,078 + Sama_0268 0.44 +0.1 308,116 + Sama_0268 0.49 +0.3 308,145 - Sama_0268 0.52 -0.9 308,181 + Sama_0268 0.56 -0.6 308,193 + Sama_0268 0.58 +1.2 308,193 + Sama_0268 0.58 +0.2 308,193 + Sama_0268 0.58 +0.5 308,193 + Sama_0268 0.58 -0.4 308,193 + Sama_0268 0.58 -0.0 308,193 + Sama_0268 0.58 +1.0 308,194 - Sama_0268 0.58 +0.4 308,194 - Sama_0268 0.58 -0.6 308,194 - Sama_0268 0.58 -0.2 308,213 + Sama_0268 0.60 -0.1 308,214 - Sama_0268 0.60 -1.8 308,214 - Sama_0268 0.60 +0.4 308,234 + Sama_0268 0.62 +0.0 308,234 + Sama_0268 0.62 -0.3 308,235 - Sama_0268 0.62 +0.4 308,235 - Sama_0268 0.62 -0.8 308,235 - Sama_0268 0.62 -1.6 308,235 - Sama_0268 0.62 -0.6 308,235 - Sama_0268 0.62 +0.5 308,235 - Sama_0268 0.62 -0.5 308,244 + Sama_0268 0.63 -0.1 308,244 + Sama_0268 0.63 -1.1 308,244 + Sama_0268 0.63 +0.3 308,244 + Sama_0268 0.63 +0.0 308,244 + Sama_0268 0.63 -0.6 308,244 + Sama_0268 0.63 +0.2 308,244 + Sama_0268 0.63 +0.8 308,244 + Sama_0268 0.63 -0.1 308,245 - Sama_0268 0.64 +0.4 308,245 - Sama_0268 0.64 +1.1 308,245 - Sama_0268 0.64 +0.3 308,245 - Sama_0268 0.64 +0.1 308,245 - Sama_0268 0.64 -0.5 308,245 - Sama_0268 0.64 -0.9 308,245 - Sama_0268 0.64 +0.3 308,265 + Sama_0268 0.66 -0.3 308,265 + Sama_0268 0.66 +0.1 308,265 + Sama_0268 0.66 -2.0 308,266 - Sama_0268 0.66 -0.2 308,266 - Sama_0268 0.66 -0.6 308,279 - Sama_0268 0.67 -0.6 308,373 + Sama_0268 0.78 -0.5 308,374 - Sama_0268 0.78 -0.8 308,374 - Sama_0268 0.78 -0.4 308,374 - Sama_0268 0.78 -0.4 308,374 - Sama_0268 0.78 +1.0 308,376 + Sama_0268 0.79 -1.0 308,377 - Sama_0268 0.79 -0.8 308,417 + Sama_0268 0.83 +0.5 308,417 + Sama_0268 0.83 -0.9 308,417 + Sama_0268 0.83 +0.2 308,417 + Sama_0268 0.83 -0.3 308,417 + Sama_0268 0.83 -0.3 308,418 - Sama_0268 0.84 -0.3 308,418 - Sama_0268 0.84 +0.3 308,418 - Sama_0268 0.84 -1.7 308,418 - Sama_0268 0.84 -0.0 308,418 - Sama_0268 0.84 -1.3 308,501 - -1.0 308,501 - -1.4 308,504 + +0.6 308,504 + -0.4 308,504 + -1.3 308,504 + -0.8 308,504 + -0.6 308,504 + -0.2 308,505 - -0.0 308,505 - -0.3 308,505 - -1.2 308,527 - -0.1 308,527 - -1.2 308,547 + +0.3 308,547 + -0.6 308,547 + -2.3 308,547 + -1.3 308,547 + -0.6 308,547 + -0.5 308,548 - -1.7 308,589 + +0.9 308,589 + +1.0
Or see this region's nucleotide sequence