Experiment: D-Cycloserine 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0014 and recF are separated by 27 nucleotides recF and Sama_0016 are separated by 92 nucleotides
Sama_0014: Sama_0014 - DNA-directed DNA polymerase (RefSeq), at 12,168 to 13,268
_0014
Sama_0015: recF - recombination protein F (RefSeq), at 13,296 to 14,378
recF
Sama_0016: Sama_0016 - DNA topoisomerase (ATP-hydrolyzing) (RefSeq), at 14,471 to 16,888
_0016
Position (kb) 13
14
15 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 13.266 kb on + strand at 13.266 kb on + strand at 13.266 kb on + strand at 13.266 kb on + strand at 13.267 kb on - strand at 13.295 kb on + strand at 13.295 kb on + strand at 13.296 kb on - strand at 13.296 kb on - strand at 13.296 kb on - strand at 13.334 kb on + strand at 13.335 kb on - strand at 13.335 kb on - strand at 13.380 kb on + strand at 13.380 kb on + strand at 13.381 kb on - strand at 13.425 kb on + strand, within recF at 13.425 kb on + strand, within recF at 13.425 kb on + strand, within recF at 13.425 kb on + strand, within recF at 13.425 kb on + strand, within recF at 13.452 kb on - strand, within recF at 13.452 kb on - strand, within recF at 13.541 kb on + strand, within recF at 13.542 kb on - strand, within recF at 13.542 kb on - strand, within recF at 13.630 kb on + strand, within recF at 13.630 kb on + strand, within recF at 13.630 kb on + strand, within recF at 13.630 kb on + strand, within recF at 13.630 kb on + strand, within recF at 13.630 kb on + strand, within recF at 13.630 kb on + strand, within recF at 13.630 kb on + strand, within recF at 13.631 kb on - strand, within recF at 13.631 kb on - strand, within recF at 13.631 kb on - strand, within recF at 13.631 kb on - strand, within recF at 13.635 kb on - strand, within recF at 13.635 kb on - strand, within recF at 13.635 kb on - strand, within recF at 13.635 kb on - strand, within recF at 13.647 kb on - strand, within recF at 13.701 kb on - strand, within recF at 13.701 kb on - strand, within recF at 13.754 kb on + strand, within recF at 13.754 kb on + strand, within recF at 13.755 kb on - strand, within recF at 13.838 kb on - strand, within recF at 13.841 kb on + strand, within recF at 13.867 kb on + strand, within recF at 13.867 kb on + strand, within recF at 13.878 kb on + strand, within recF at 13.878 kb on + strand, within recF at 13.878 kb on + strand, within recF at 13.878 kb on + strand, within recF at 13.878 kb on + strand, within recF at 13.879 kb on - strand, within recF at 13.879 kb on - strand, within recF at 13.879 kb on - strand, within recF at 13.882 kb on + strand, within recF at 13.882 kb on + strand, within recF at 13.882 kb on + strand, within recF at 13.882 kb on + strand, within recF at 13.883 kb on - strand, within recF at 13.883 kb on - strand, within recF at 13.883 kb on - strand, within recF at 13.883 kb on - strand, within recF at 13.883 kb on - strand, within recF at 13.883 kb on - strand, within recF at 13.904 kb on + strand, within recF at 13.904 kb on + strand, within recF at 13.904 kb on + strand, within recF at 13.910 kb on + strand, within recF at 13.910 kb on + strand, within recF at 13.912 kb on + strand, within recF at 13.912 kb on + strand, within recF at 14.007 kb on + strand, within recF at 14.026 kb on + strand, within recF at 14.033 kb on + strand, within recF at 14.033 kb on + strand, within recF at 14.051 kb on + strand, within recF at 14.051 kb on + strand, within recF at 14.078 kb on + strand, within recF at 14.078 kb on + strand, within recF at 14.122 kb on + strand, within recF at 14.162 kb on + strand, within recF at 14.162 kb on + strand, within recF at 14.162 kb on + strand, within recF at 14.190 kb on + strand, within recF at 14.190 kb on + strand, within recF at 14.190 kb on + strand, within recF at 14.190 kb on + strand, within recF at 14.190 kb on + strand, within recF at 14.257 kb on + strand, within recF at 14.257 kb on + strand, within recF at 14.257 kb on + strand, within recF at 14.261 kb on + strand, within recF at 14.261 kb on + strand, within recF at 14.347 kb on + strand at 14.347 kb on + strand at 14.347 kb on + strand at 14.372 kb on + strand at 14.372 kb on + strand at 14.372 kb on + strand at 14.372 kb on + strand at 14.376 kb on + strand at 14.376 kb on + strand at 14.376 kb on + strand at 14.376 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.03125 mg/ml remove 13,266 + -2.6 13,266 + -0.1 13,266 + -1.6 13,266 + -2.4 13,267 - -0.8 13,295 + -1.0 13,295 + +0.6 13,296 - -0.2 13,296 - -1.4 13,296 - -1.4 13,334 + -2.1 13,335 - -2.8 13,335 - -0.2 13,380 + -2.4 13,380 + -2.8 13,381 - -1.4 13,425 + recF Sama_0015 0.12 +0.6 13,425 + recF Sama_0015 0.12 -3.3 13,425 + recF Sama_0015 0.12 +0.1 13,425 + recF Sama_0015 0.12 -2.2 13,425 + recF Sama_0015 0.12 -1.2 13,452 - recF Sama_0015 0.14 -1.6 13,452 - recF Sama_0015 0.14 -2.7 13,541 + recF Sama_0015 0.23 -0.8 13,542 - recF Sama_0015 0.23 -2.1 13,542 - recF Sama_0015 0.23 -2.7 13,630 + recF Sama_0015 0.31 -1.4 13,630 + recF Sama_0015 0.31 -1.4 13,630 + recF Sama_0015 0.31 +0.5 13,630 + recF Sama_0015 0.31 -2.0 13,630 + recF Sama_0015 0.31 +0.2 13,630 + recF Sama_0015 0.31 -1.8 13,630 + recF Sama_0015 0.31 -3.3 13,630 + recF Sama_0015 0.31 -1.0 13,631 - recF Sama_0015 0.31 -3.0 13,631 - recF Sama_0015 0.31 -3.3 13,631 - recF Sama_0015 0.31 -0.9 13,631 - recF Sama_0015 0.31 -0.4 13,635 - recF Sama_0015 0.31 -1.1 13,635 - recF Sama_0015 0.31 -1.5 13,635 - recF Sama_0015 0.31 -2.5 13,635 - recF Sama_0015 0.31 -2.6 13,647 - recF Sama_0015 0.32 -0.4 13,701 - recF Sama_0015 0.37 -1.4 13,701 - recF Sama_0015 0.37 -1.5 13,754 + recF Sama_0015 0.42 -1.3 13,754 + recF Sama_0015 0.42 -2.2 13,755 - recF Sama_0015 0.42 -1.0 13,838 - recF Sama_0015 0.50 -0.2 13,841 + recF Sama_0015 0.50 -1.4 13,867 + recF Sama_0015 0.53 -1.5 13,867 + recF Sama_0015 0.53 -2.4 13,878 + recF Sama_0015 0.54 -3.1 13,878 + recF Sama_0015 0.54 -0.2 13,878 + recF Sama_0015 0.54 -1.7 13,878 + recF Sama_0015 0.54 -1.0 13,878 + recF Sama_0015 0.54 -1.9 13,879 - recF Sama_0015 0.54 -1.4 13,879 - recF Sama_0015 0.54 -3.4 13,879 - recF Sama_0015 0.54 -3.6 13,882 + recF Sama_0015 0.54 -1.9 13,882 + recF Sama_0015 0.54 -1.0 13,882 + recF Sama_0015 0.54 -1.1 13,882 + recF Sama_0015 0.54 -1.4 13,883 - recF Sama_0015 0.54 -2.0 13,883 - recF Sama_0015 0.54 -0.4 13,883 - recF Sama_0015 0.54 -1.9 13,883 - recF Sama_0015 0.54 +1.2 13,883 - recF Sama_0015 0.54 -1.7 13,883 - recF Sama_0015 0.54 -1.7 13,904 + recF Sama_0015 0.56 -1.8 13,904 + recF Sama_0015 0.56 -0.4 13,904 + recF Sama_0015 0.56 -1.5 13,910 + recF Sama_0015 0.57 -0.9 13,910 + recF Sama_0015 0.57 -0.8 13,912 + recF Sama_0015 0.57 -1.0 13,912 + recF Sama_0015 0.57 -0.5 14,007 + recF Sama_0015 0.66 -2.6 14,026 + recF Sama_0015 0.67 -0.6 14,033 + recF Sama_0015 0.68 -2.0 14,033 + recF Sama_0015 0.68 -1.0 14,051 + recF Sama_0015 0.70 -1.0 14,051 + recF Sama_0015 0.70 -0.9 14,078 + recF Sama_0015 0.72 +1.8 14,078 + recF Sama_0015 0.72 -1.7 14,122 + recF Sama_0015 0.76 -3.2 14,162 + recF Sama_0015 0.80 -1.5 14,162 + recF Sama_0015 0.80 -0.8 14,162 + recF Sama_0015 0.80 -1.9 14,190 + recF Sama_0015 0.83 -0.8 14,190 + recF Sama_0015 0.83 -1.7 14,190 + recF Sama_0015 0.83 -2.0 14,190 + recF Sama_0015 0.83 -1.9 14,190 + recF Sama_0015 0.83 -1.5 14,257 + recF Sama_0015 0.89 -0.1 14,257 + recF Sama_0015 0.89 -1.0 14,257 + recF Sama_0015 0.89 -3.0 14,261 + recF Sama_0015 0.89 -0.4 14,261 + recF Sama_0015 0.89 -1.7 14,347 + -1.9 14,347 + -0.7 14,347 + -1.1 14,372 + -0.8 14,372 + +1.1 14,372 + -0.2 14,372 + +0.5 14,376 + -1.2 14,376 + -0.6 14,376 + -1.6 14,376 + -2.2
Or see this region's nucleotide sequence