Experiment: A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2251 and SO2253 are separated by 572 nucleotides SO2253 and SO2254 are separated by 120 nucleotides SO2254 and mfd overlap by 1 nucleotides
SO2251: SO2251 - conserved hypothetical protein (NCBI ptt file), at 2,365,344 to 2,365,883
SO2251
SO2253: SO2253 - acylphosphatase (NCBI ptt file), at 2,366,456 to 2,366,728
SO2253
SO2254: SO2254 - conserved hypothetical protein (NCBI ptt file), at 2,366,849 to 2,367,847
SO2254
SO2255: mfd - transcription-repair coupling factor (NCBI ptt file), at 2,367,847 to 2,371,341
mfd
Position (kb)
2366
2367
2368 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2365.875 kb on - strand at 2365.875 kb on - strand at 2365.885 kb on - strand at 2365.909 kb on - strand at 2366.024 kb on + strand at 2366.303 kb on - strand at 2366.407 kb on + strand at 2366.422 kb on + strand at 2366.489 kb on + strand, within SO2253 at 2366.494 kb on + strand, within SO2253 at 2366.661 kb on + strand, within SO2253 at 2366.762 kb on + strand at 2367.220 kb on - strand, within SO2254 at 2367.303 kb on + strand, within SO2254 at 2367.311 kb on - strand, within SO2254 at 2367.311 kb on - strand, within SO2254 at 2367.330 kb on + strand, within SO2254 at 2367.496 kb on + strand, within SO2254 at 2367.734 kb on + strand, within SO2254 at 2367.797 kb on + strand at 2368.039 kb on - strand at 2368.117 kb on - strand at 2368.192 kb on + strand at 2368.440 kb on - strand, within mfd
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.01 mg/ml remove 2,365,875 - -0.1 2,365,875 - -0.4 2,365,885 - +0.3 2,365,909 - +0.6 2,366,024 + -0.8 2,366,303 - -0.0 2,366,407 + +0.1 2,366,422 + -0.6 2,366,489 + SO2253 0.12 +0.3 2,366,494 + SO2253 0.14 -0.3 2,366,661 + SO2253 0.75 +0.4 2,366,762 + -0.8 2,367,220 - SO2254 0.37 +1.2 2,367,303 + SO2254 0.45 +0.5 2,367,311 - SO2254 0.46 +0.7 2,367,311 - SO2254 0.46 +0.6 2,367,330 + SO2254 0.48 +0.7 2,367,496 + SO2254 0.65 +0.8 2,367,734 + SO2254 0.89 +0.6 2,367,797 + +2.6 2,368,039 - -0.0 2,368,117 - +1.7 2,368,192 + -0.1 2,368,440 - mfd SO2255 0.17 -2.0
Or see this region's nucleotide sequence