Experiment: A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dacA-1 and SO1165 are separated by 144 nucleotides SO1165 and SO1166 overlap by 17 nucleotides SO1166 and rodA are separated by 16 nucleotides
SO1164: dacA-1 - D-alanyl-D-alanine carboxypeptidase (NCBI ptt file), at 1,206,370 to 1,207,542
dacA-1
SO1165: SO1165 - rare lipoprotein A (NCBI ptt file), at 1,207,687 to 1,208,538
SO1165
SO1166: SO1166 - membrane-bound lytic transglycosylase, putative (NCBI ptt file), at 1,208,522 to 1,209,586
SO1166
SO1167: rodA - rod shape-determining protein RodA (NCBI ptt file), at 1,209,603 to 1,210,721
rodA
Position (kb)
1208
1209
1210 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1207.729 kb on - strand at 1207.746 kb on - strand at 1207.809 kb on + strand, within SO1165 at 1207.910 kb on + strand, within SO1165 at 1207.949 kb on - strand, within SO1165 at 1208.027 kb on + strand, within SO1165 at 1208.040 kb on - strand, within SO1165 at 1208.118 kb on + strand, within SO1165 at 1208.128 kb on + strand, within SO1165 at 1208.130 kb on - strand, within SO1165 at 1208.186 kb on + strand, within SO1165 at 1208.194 kb on - strand, within SO1165 at 1208.196 kb on + strand, within SO1165 at 1208.238 kb on - strand, within SO1165 at 1208.301 kb on + strand, within SO1165 at 1208.302 kb on - strand, within SO1165 at 1208.302 kb on - strand, within SO1165 at 1208.370 kb on + strand, within SO1165 at 1208.956 kb on - strand, within SO1166 at 1208.963 kb on - strand, within SO1166 at 1208.994 kb on + strand, within SO1166 at 1209.002 kb on - strand, within SO1166 at 1209.032 kb on - strand, within SO1166 at 1209.060 kb on - strand, within SO1166 at 1209.098 kb on + strand, within SO1166 at 1209.106 kb on - strand, within SO1166 at 1209.462 kb on - strand, within SO1166 at 1209.477 kb on - strand, within SO1166 at 1209.495 kb on - strand at 1209.500 kb on - strand at 1209.537 kb on - strand at 1209.628 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.01 mg/ml remove 1,207,729 - +0.7 1,207,746 - -0.2 1,207,809 + SO1165 0.14 -2.8 1,207,910 + SO1165 0.26 -1.0 1,207,949 - SO1165 0.31 -0.4 1,208,027 + SO1165 0.40 -0.2 1,208,040 - SO1165 0.41 -0.8 1,208,118 + SO1165 0.51 -1.7 1,208,128 + SO1165 0.52 -0.7 1,208,130 - SO1165 0.52 -1.5 1,208,186 + SO1165 0.59 -1.6 1,208,194 - SO1165 0.60 -0.6 1,208,196 + SO1165 0.60 -0.3 1,208,238 - SO1165 0.65 -1.6 1,208,301 + SO1165 0.72 -0.6 1,208,302 - SO1165 0.72 -0.8 1,208,302 - SO1165 0.72 -0.8 1,208,370 + SO1165 0.80 -0.5 1,208,956 - SO1166 0.41 -0.8 1,208,963 - SO1166 0.41 -0.6 1,208,994 + SO1166 0.44 -0.7 1,209,002 - SO1166 0.45 -1.0 1,209,032 - SO1166 0.48 -0.5 1,209,060 - SO1166 0.51 -0.7 1,209,098 + SO1166 0.54 -0.9 1,209,106 - SO1166 0.55 +0.1 1,209,462 - SO1166 0.88 -2.8 1,209,477 - SO1166 0.90 -2.4 1,209,495 - -0.6 1,209,500 - -1.2 1,209,537 - -1.0 1,209,628 - -0.7
Or see this region's nucleotide sequence