Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_78p00170 and PGA1_78p00180 overlap by 8 nucleotides PGA1_78p00180 and PGA1_78p00190 are separated by 6 nucleotides PGA1_78p00190 and PGA1_78p00200 are separated by 20 nucleotides PGA1_78p00200 and PGA1_78p00210 are separated by 9 nucleotides
PGA1_78p00170: PGA1_78p00170 - putative sn-glycerol-3-phosphate transport system permease protein UgpA, at 20,341 to 21,273
_78p00170
PGA1_78p00180: PGA1_78p00180 - putative sn-glycerol-3-phosphate transport system permease protein UgpE, at 21,266 to 22,195
_78p00180
PGA1_78p00190: PGA1_78p00190 - sn-glycerol-3-phosphate import ATP-binding protein UgbC, at 22,202 to 23,287
_78p00190
PGA1_78p00200: PGA1_78p00200 - Sugar phosphate isomerases/epimerases, at 23,308 to 24,051
_78p00200
PGA1_78p00210: PGA1_78p00210 - putative oxidoreductase, at 24,061 to 25,194
_78p00210
Position (kb)
22
23
24 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 21.439 kb on + strand, within PGA1_78p00180 at 21.517 kb on + strand, within PGA1_78p00180 at 21.554 kb on + strand, within PGA1_78p00180 at 21.753 kb on + strand, within PGA1_78p00180 at 22.116 kb on + strand at 22.192 kb on - strand at 22.427 kb on - strand, within PGA1_78p00190 at 22.499 kb on + strand, within PGA1_78p00190 at 22.861 kb on - strand, within PGA1_78p00190 at 23.154 kb on - strand, within PGA1_78p00190 at 23.781 kb on - strand, within PGA1_78p00200 at 23.786 kb on - strand, within PGA1_78p00200 at 24.221 kb on + strand, within PGA1_78p00210
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 21,439 + PGA1_78p00180 0.19 -0.8 21,517 + PGA1_78p00180 0.27 +0.4 21,554 + PGA1_78p00180 0.31 -0.5 21,753 + PGA1_78p00180 0.52 -0.3 22,116 + -0.9 22,192 - +0.6 22,427 - PGA1_78p00190 0.21 +4.5 22,499 + PGA1_78p00190 0.27 +2.3 22,861 - PGA1_78p00190 0.61 +1.4 23,154 - PGA1_78p00190 0.88 +0.4 23,781 - PGA1_78p00200 0.64 +0.5 23,786 - PGA1_78p00200 0.64 +1.1 24,221 + PGA1_78p00210 0.14 +0.7
Or see this region's nucleotide sequence