Experiment: A22 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppiA and SO3813 are separated by 66 nucleotides SO3813 and SO3814 are separated by 156 nucleotides SO3814 and SO3815 are separated by 106 nucleotides SO3815 and SO3816 are separated by 162 nucleotides
SO3812: ppiA - peptidyl-prolyl cis-trans isomerase A (NCBI ptt file), at 3,964,412 to 3,964,999
ppiA
SO3813: SO3813 - hypothetical protein (NCBI ptt file), at 3,965,066 to 3,965,386
SO3813
SO3814: SO3814 - ampG protein, putative (NCBI ptt file), at 3,965,543 to 3,966,928
SO3814
SO3815: SO3815 - conserved hypothetical protein (NCBI ptt file), at 3,967,035 to 3,967,520
SO3815
SO3816: SO3816 - conserved hypothetical protein (NCBI ptt file), at 3,967,683 to 3,968,045
SO3816
Position (kb)
3965
3966
3967 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3964.579 kb on - strand, within ppiA at 3964.594 kb on + strand, within ppiA at 3964.594 kb on + strand, within ppiA at 3964.602 kb on - strand, within ppiA at 3964.602 kb on - strand, within ppiA at 3964.699 kb on + strand, within ppiA at 3964.729 kb on + strand, within ppiA at 3964.815 kb on + strand, within ppiA at 3964.815 kb on + strand, within ppiA at 3964.820 kb on + strand, within ppiA at 3964.823 kb on - strand, within ppiA at 3964.824 kb on - strand, within ppiA at 3964.918 kb on - strand, within ppiA at 3964.933 kb on + strand, within ppiA at 3965.156 kb on + strand, within SO3813 at 3965.178 kb on + strand, within SO3813 at 3965.178 kb on + strand, within SO3813 at 3965.178 kb on + strand, within SO3813 at 3965.180 kb on + strand, within SO3813 at 3965.186 kb on - strand, within SO3813 at 3965.188 kb on - strand, within SO3813 at 3965.373 kb on + strand at 3965.388 kb on - strand at 3965.468 kb on + strand at 3965.474 kb on + strand at 3965.482 kb on - strand at 3965.482 kb on - strand at 3965.512 kb on - strand at 3965.571 kb on + strand at 3965.578 kb on - strand at 3965.579 kb on - strand at 3965.580 kb on + strand at 3965.588 kb on + strand at 3965.588 kb on - strand at 3965.732 kb on + strand, within SO3814 at 3965.740 kb on - strand, within SO3814 at 3965.754 kb on - strand, within SO3814 at 3965.803 kb on + strand, within SO3814 at 3965.848 kb on - strand, within SO3814 at 3966.029 kb on + strand, within SO3814 at 3966.029 kb on + strand, within SO3814 at 3966.037 kb on - strand, within SO3814 at 3966.137 kb on - strand, within SO3814 at 3966.217 kb on + strand, within SO3814 at 3966.420 kb on - strand, within SO3814 at 3966.484 kb on + strand, within SO3814 at 3966.506 kb on + strand, within SO3814 at 3966.506 kb on + strand, within SO3814 at 3966.514 kb on - strand, within SO3814 at 3966.514 kb on - strand, within SO3814 at 3966.514 kb on - strand, within SO3814 at 3966.514 kb on - strand, within SO3814 at 3966.514 kb on - strand, within SO3814 at 3966.524 kb on - strand, within SO3814 at 3966.538 kb on - strand, within SO3814 at 3966.575 kb on + strand, within SO3814 at 3966.576 kb on - strand, within SO3814 at 3966.608 kb on + strand, within SO3814 at 3966.728 kb on - strand, within SO3814 at 3966.843 kb on + strand at 3966.845 kb on + strand at 3966.904 kb on - strand at 3966.906 kb on + strand at 3966.959 kb on + strand at 3967.119 kb on + strand, within SO3815 at 3967.127 kb on - strand, within SO3815 at 3967.147 kb on + strand, within SO3815 at 3967.155 kb on - strand, within SO3815 at 3967.166 kb on + strand, within SO3815 at 3967.315 kb on - strand, within SO3815 at 3967.317 kb on + strand, within SO3815 at 3967.437 kb on + strand, within SO3815 at 3967.557 kb on - strand at 3967.592 kb on - strand at 3967.592 kb on - strand at 3967.659 kb on + strand at 3967.700 kb on - strand at 3967.720 kb on - strand, within SO3816 at 3967.720 kb on - strand, within SO3816 at 3967.928 kb on - strand, within SO3816
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.003 mg/ml remove 3,964,579 - ppiA SO3812 0.28 +0.5 3,964,594 + ppiA SO3812 0.31 -0.5 3,964,594 + ppiA SO3812 0.31 -1.3 3,964,602 - ppiA SO3812 0.32 -0.4 3,964,602 - ppiA SO3812 0.32 +0.7 3,964,699 + ppiA SO3812 0.49 -2.3 3,964,729 + ppiA SO3812 0.54 +0.0 3,964,815 + ppiA SO3812 0.69 -0.5 3,964,815 + ppiA SO3812 0.69 +1.3 3,964,820 + ppiA SO3812 0.69 -0.8 3,964,823 - ppiA SO3812 0.70 -0.1 3,964,824 - ppiA SO3812 0.70 +0.6 3,964,918 - ppiA SO3812 0.86 +0.2 3,964,933 + ppiA SO3812 0.89 +0.2 3,965,156 + SO3813 0.28 -0.4 3,965,178 + SO3813 0.35 +0.4 3,965,178 + SO3813 0.35 -0.6 3,965,178 + SO3813 0.35 +0.2 3,965,180 + SO3813 0.36 +0.0 3,965,186 - SO3813 0.37 -0.4 3,965,188 - SO3813 0.38 +0.1 3,965,373 + -0.5 3,965,388 - -0.3 3,965,468 + +0.2 3,965,474 + +0.3 3,965,482 - -0.4 3,965,482 - +0.3 3,965,512 - -0.6 3,965,571 + -0.3 3,965,578 - -1.0 3,965,579 - -0.9 3,965,580 + -1.1 3,965,588 + -0.9 3,965,588 - -0.9 3,965,732 + SO3814 0.14 -1.1 3,965,740 - SO3814 0.14 -2.8 3,965,754 - SO3814 0.15 -1.0 3,965,803 + SO3814 0.19 -1.1 3,965,848 - SO3814 0.22 -1.1 3,966,029 + SO3814 0.35 -0.6 3,966,029 + SO3814 0.35 +0.0 3,966,037 - SO3814 0.36 -1.0 3,966,137 - SO3814 0.43 +0.1 3,966,217 + SO3814 0.49 -1.0 3,966,420 - SO3814 0.63 -1.6 3,966,484 + SO3814 0.68 -0.8 3,966,506 + SO3814 0.69 -0.8 3,966,506 + SO3814 0.69 -0.8 3,966,514 - SO3814 0.70 -1.3 3,966,514 - SO3814 0.70 -1.2 3,966,514 - SO3814 0.70 -0.6 3,966,514 - SO3814 0.70 -1.4 3,966,514 - SO3814 0.70 -0.6 3,966,524 - SO3814 0.71 -1.3 3,966,538 - SO3814 0.72 -0.5 3,966,575 + SO3814 0.74 -1.0 3,966,576 - SO3814 0.75 -1.1 3,966,608 + SO3814 0.77 -0.7 3,966,728 - SO3814 0.85 -1.5 3,966,843 + -0.9 3,966,845 + -0.9 3,966,904 - -1.4 3,966,906 + -0.5 3,966,959 + +0.8 3,967,119 + SO3815 0.17 +0.2 3,967,127 - SO3815 0.19 +0.1 3,967,147 + SO3815 0.23 +0.6 3,967,155 - SO3815 0.25 +0.1 3,967,166 + SO3815 0.27 -0.6 3,967,315 - SO3815 0.58 +0.4 3,967,317 + SO3815 0.58 +1.0 3,967,437 + SO3815 0.83 -0.3 3,967,557 - -0.2 3,967,592 - +0.0 3,967,592 - -0.5 3,967,659 + -0.0 3,967,700 - -0.3 3,967,720 - SO3816 0.10 -0.2 3,967,720 - SO3816 0.10 +0.1 3,967,928 - SO3816 0.67 +0.2
Or see this region's nucleotide sequence