Experiment: A22 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt holB and tmk overlap by 7 nucleotides tmk and SO2614 overlap by 4 nucleotides SO2614 and pabC overlap by 4 nucleotides pabC and dcd are separated by 5 nucleotides
SO2612: holB - DNA polymerase III, delta prime subunit (NCBI ptt file), at 2,748,778 to 2,749,734
holB
SO2613: tmk - thymidylate kinase (NCBI ptt file), at 2,749,728 to 2,750,357
tmk
SO2614: SO2614 - conserved hypothetical protein TIGR00247 (NCBI ptt file), at 2,750,354 to 2,751,364
SO2614
SO2615: pabC - 4-amino-4-deoxychorismate lyase (NCBI ptt file), at 2,751,361 to 2,752,170
pabC
SO2616: dcd - deoxycytidine triphosphate deaminase (NCBI ptt file), at 2,752,176 to 2,752,757
dcd
Position (kb)
2750
2751
2752 Strain fitness (log2 ratio)
-2
-1
0
1 at 2750.398 kb on - strand at 2750.406 kb on - strand at 2750.552 kb on - strand, within SO2614 at 2750.552 kb on - strand, within SO2614 at 2750.572 kb on - strand, within SO2614 at 2750.598 kb on - strand, within SO2614 at 2750.704 kb on - strand, within SO2614 at 2751.419 kb on - strand at 2751.419 kb on - strand at 2751.555 kb on - strand, within pabC at 2751.734 kb on - strand, within pabC at 2751.758 kb on - strand, within pabC at 2751.948 kb on - strand, within pabC
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.003 mg/ml remove 2,750,398 - -0.5 2,750,406 - +0.7 2,750,552 - SO2614 0.20 -1.7 2,750,552 - SO2614 0.20 -0.3 2,750,572 - SO2614 0.22 -0.0 2,750,598 - SO2614 0.24 -1.3 2,750,704 - SO2614 0.35 -2.3 2,751,419 - +0.5 2,751,419 - -1.2 2,751,555 - pabC SO2615 0.24 +0.9 2,751,734 - pabC SO2615 0.46 -1.5 2,751,758 - pabC SO2615 0.49 -0.2 2,751,948 - pabC SO2615 0.72 -0.6
Or see this region's nucleotide sequence