Experiment: Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3562 and SO3563 are separated by 67 nucleotides SO3563 and dcp-2 are separated by 220 nucleotides dcp-2 and cpdB are separated by 683 nucleotides
SO3562: SO3562 - proton/glutamate symporter, putative (NCBI ptt file), at 3,722,583 to 3,723,824
SO3562
SO3563: SO3563 - SM-20 domain protein (NCBI ptt file), at 3,723,892 to 3,724,515
SO3563
SO3564: dcp-2 - peptidyl-dipeptidase Dcp (NCBI ptt file), at 3,724,736 to 3,726,886
dcp-2
SO3565: cpdB - 2',3'-cyclic-nucleotide 2'-phosphodiesterase (NCBI ptt file), at 3,727,570 to 3,729,627
cpdB
Position (kb)
3724
3725
3726
3727 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7 at 3723.786 kb on - strand at 3723.786 kb on - strand at 3723.796 kb on - strand at 3723.796 kb on - strand at 3723.796 kb on - strand at 3723.800 kb on + strand at 3723.808 kb on - strand at 3723.831 kb on - strand at 3723.918 kb on + strand at 3723.918 kb on + strand at 3723.918 kb on + strand at 3723.918 kb on + strand at 3723.926 kb on - strand at 3724.062 kb on + strand, within SO3563 at 3724.062 kb on + strand, within SO3563 at 3724.277 kb on + strand, within SO3563 at 3724.285 kb on - strand, within SO3563 at 3724.289 kb on - strand, within SO3563 at 3724.338 kb on + strand, within SO3563 at 3724.338 kb on + strand, within SO3563 at 3724.350 kb on + strand, within SO3563 at 3724.454 kb on + strand at 3724.800 kb on + strand at 3724.846 kb on - strand at 3724.875 kb on - strand at 3724.881 kb on + strand at 3724.886 kb on + strand at 3724.932 kb on - strand at 3724.993 kb on + strand, within dcp-2 at 3725.051 kb on - strand, within dcp-2 at 3725.111 kb on + strand, within dcp-2 at 3725.273 kb on - strand, within dcp-2 at 3725.349 kb on - strand, within dcp-2 at 3725.380 kb on + strand, within dcp-2 at 3725.389 kb on - strand, within dcp-2 at 3725.442 kb on + strand, within dcp-2 at 3725.460 kb on - strand, within dcp-2 at 3725.522 kb on - strand, within dcp-2 at 3725.588 kb on + strand, within dcp-2 at 3725.593 kb on + strand, within dcp-2 at 3725.603 kb on + strand, within dcp-2 at 3725.661 kb on + strand, within dcp-2 at 3725.669 kb on - strand, within dcp-2 at 3725.747 kb on + strand, within dcp-2 at 3725.931 kb on - strand, within dcp-2 at 3725.941 kb on + strand, within dcp-2 at 3726.018 kb on + strand, within dcp-2 at 3726.025 kb on + strand, within dcp-2 at 3726.055 kb on + strand, within dcp-2 at 3726.055 kb on + strand, within dcp-2 at 3726.123 kb on + strand, within dcp-2 at 3726.284 kb on - strand, within dcp-2 at 3726.284 kb on - strand, within dcp-2 at 3726.497 kb on + strand, within dcp-2 at 3726.654 kb on - strand, within dcp-2 at 3726.658 kb on + strand, within dcp-2 at 3726.707 kb on + strand at 3726.716 kb on - strand at 3726.736 kb on + strand at 3726.744 kb on - strand at 3726.755 kb on + strand at 3726.755 kb on + strand at 3726.755 kb on + strand at 3726.763 kb on - strand at 3726.784 kb on - strand at 3726.800 kb on - strand at 3726.901 kb on + strand at 3726.901 kb on + strand at 3726.909 kb on - strand at 3726.993 kb on + strand at 3727.006 kb on + strand at 3727.016 kb on + strand at 3727.016 kb on + strand at 3727.016 kb on + strand at 3727.024 kb on - strand at 3727.080 kb on - strand at 3727.080 kb on - strand at 3727.080 kb on - strand at 3727.115 kb on + strand at 3727.115 kb on + strand at 3727.123 kb on - strand at 3727.356 kb on - strand at 3727.376 kb on + strand at 3727.400 kb on + strand at 3727.456 kb on + strand at 3727.461 kb on + strand at 3727.469 kb on + strand at 3727.469 kb on + strand at 3727.476 kb on + strand at 3727.476 kb on + strand at 3727.477 kb on - strand at 3727.477 kb on - strand at 3727.484 kb on - strand at 3727.489 kb on - strand at 3727.506 kb on - strand at 3727.604 kb on + strand at 3727.604 kb on - strand at 3727.604 kb on - strand at 3727.612 kb on - strand at 3727.612 kb on - strand at 3727.624 kb on - strand at 3727.679 kb on + strand at 3727.679 kb on + strand at 3727.687 kb on - strand at 3727.763 kb on + strand at 3727.777 kb on + strand, within cpdB at 3727.824 kb on + strand, within cpdB at 3727.824 kb on + strand, within cpdB at 3727.825 kb on + strand, within cpdB at 3727.832 kb on - strand, within cpdB at 3727.832 kb on - strand, within cpdB at 3727.832 kb on - strand, within cpdB at 3727.832 kb on - strand, within cpdB at 3727.833 kb on - strand, within cpdB
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.06 mg/ml remove 3,723,786 - +0.1 3,723,786 - -0.2 3,723,796 - -0.2 3,723,796 - +0.3 3,723,796 - +0.4 3,723,800 + -0.6 3,723,808 - -0.4 3,723,831 - -0.3 3,723,918 + -1.5 3,723,918 + -0.6 3,723,918 + +0.2 3,723,918 + -0.5 3,723,926 - -0.4 3,724,062 + SO3563 0.27 -0.2 3,724,062 + SO3563 0.27 -0.4 3,724,277 + SO3563 0.62 +0.6 3,724,285 - SO3563 0.63 -0.6 3,724,289 - SO3563 0.64 -3.2 3,724,338 + SO3563 0.71 +0.6 3,724,338 + SO3563 0.71 +0.4 3,724,350 + SO3563 0.73 +0.3 3,724,454 + +0.1 3,724,800 + -0.4 3,724,846 - -0.0 3,724,875 - +0.5 3,724,881 + +0.1 3,724,886 + +0.5 3,724,932 - +0.2 3,724,993 + dcp-2 SO3564 0.12 -0.2 3,725,051 - dcp-2 SO3564 0.15 +0.4 3,725,111 + dcp-2 SO3564 0.17 -0.4 3,725,273 - dcp-2 SO3564 0.25 +1.3 3,725,349 - dcp-2 SO3564 0.28 -3.4 3,725,380 + dcp-2 SO3564 0.30 -0.2 3,725,389 - dcp-2 SO3564 0.30 +0.6 3,725,442 + dcp-2 SO3564 0.33 -0.4 3,725,460 - dcp-2 SO3564 0.34 +6.8 3,725,522 - dcp-2 SO3564 0.37 -0.8 3,725,588 + dcp-2 SO3564 0.40 +0.2 3,725,593 + dcp-2 SO3564 0.40 +0.7 3,725,603 + dcp-2 SO3564 0.40 +0.0 3,725,661 + dcp-2 SO3564 0.43 +0.8 3,725,669 - dcp-2 SO3564 0.43 +0.3 3,725,747 + dcp-2 SO3564 0.47 -0.5 3,725,931 - dcp-2 SO3564 0.56 -0.6 3,725,941 + dcp-2 SO3564 0.56 -0.1 3,726,018 + dcp-2 SO3564 0.60 -0.2 3,726,025 + dcp-2 SO3564 0.60 +0.3 3,726,055 + dcp-2 SO3564 0.61 +0.1 3,726,055 + dcp-2 SO3564 0.61 -1.6 3,726,123 + dcp-2 SO3564 0.64 -0.3 3,726,284 - dcp-2 SO3564 0.72 +0.2 3,726,284 - dcp-2 SO3564 0.72 +0.4 3,726,497 + dcp-2 SO3564 0.82 +0.1 3,726,654 - dcp-2 SO3564 0.89 +0.1 3,726,658 + dcp-2 SO3564 0.89 -0.6 3,726,707 + +0.6 3,726,716 - -0.2 3,726,736 + +0.0 3,726,744 - -0.1 3,726,755 + -2.1 3,726,755 + -0.8 3,726,755 + -1.6 3,726,763 - -0.2 3,726,784 - -0.4 3,726,800 - -0.3 3,726,901 + +0.4 3,726,901 + +0.9 3,726,909 - -0.4 3,726,993 + -0.1 3,727,006 + -0.5 3,727,016 + -0.9 3,727,016 + +0.8 3,727,016 + -1.4 3,727,024 - -0.8 3,727,080 - +0.3 3,727,080 - -0.0 3,727,080 - +0.1 3,727,115 + +1.0 3,727,115 + -0.1 3,727,123 - -1.9 3,727,356 - -0.5 3,727,376 + -1.6 3,727,400 + -0.3 3,727,456 + -1.0 3,727,461 + -0.3 3,727,469 + -0.5 3,727,469 + -0.1 3,727,476 + -1.9 3,727,476 + +0.4 3,727,477 - +1.9 3,727,477 - +0.6 3,727,484 - -1.6 3,727,489 - +0.2 3,727,506 - -1.3 3,727,604 + -0.3 3,727,604 - +0.6 3,727,604 - -0.2 3,727,612 - +0.3 3,727,612 - -0.6 3,727,624 - -0.0 3,727,679 + +0.3 3,727,679 + -0.1 3,727,687 - -0.3 3,727,763 + -0.8 3,727,777 + cpdB SO3565 0.10 +0.1 3,727,824 + cpdB SO3565 0.12 +0.2 3,727,824 + cpdB SO3565 0.12 -1.1 3,727,825 + cpdB SO3565 0.12 -1.8 3,727,832 - cpdB SO3565 0.13 +0.3 3,727,832 - cpdB SO3565 0.13 -0.4 3,727,832 - cpdB SO3565 0.13 -0.3 3,727,832 - cpdB SO3565 0.13 -0.1 3,727,833 - cpdB SO3565 0.13 -0.4
Or see this region's nucleotide sequence