Experiment: Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2772 and SO2773 are separated by 116 nucleotides SO2773 and fabF-1 are separated by 177 nucleotides fabF-1 and acpP are separated by 209 nucleotides acpP and fabG-1 are separated by 163 nucleotides
SO2772: SO2772 - cytosolic long-chain acyl-CoA thioester hydrolase family protein (NCBI ptt file), at 2,895,188 to 2,895,667
SO2772
SO2773: SO2773 - hypothetical protein (NCBI ptt file), at 2,895,784 to 2,896,152
SO2773
SO2774: fabF-1 - 3-oxoacyl-(acyl-carrier-protein) synthase II (NCBI ptt file), at 2,896,330 to 2,897,568
fabF-1
SO2775: acpP - acyl carrier protein (NCBI ptt file), at 2,897,778 to 2,898,011
acpP
SO2776: fabG-1 - 3-oxoacyl-(acyl-carrier-protein) reductase (NCBI ptt file), at 2,898,175 to 2,898,921
fabG-1
Position (kb)
2896
2897
2898 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2895.530 kb on + strand, within SO2772 at 2895.548 kb on + strand, within SO2772 at 2895.548 kb on + strand, within SO2772 at 2895.548 kb on + strand, within SO2772 at 2895.556 kb on - strand, within SO2772 at 2895.556 kb on - strand, within SO2772 at 2895.588 kb on + strand, within SO2772 at 2895.938 kb on - strand, within SO2773 at 2895.992 kb on + strand, within SO2773 at 2896.128 kb on - strand at 2896.381 kb on + strand at 2896.392 kb on - strand at 2896.927 kb on - strand, within fabF-1 at 2896.979 kb on - strand, within fabF-1 at 2897.280 kb on - strand, within fabF-1 at 2897.285 kb on - strand, within fabF-1 at 2897.301 kb on - strand, within fabF-1 at 2897.360 kb on - strand, within fabF-1 at 2897.473 kb on - strand at 2897.485 kb on - strand at 2897.532 kb on + strand at 2897.600 kb on + strand at 2898.140 kb on + strand at 2898.180 kb on - strand at 2898.180 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.06 mg/ml remove 2,895,530 + SO2772 0.71 -1.6 2,895,548 + SO2772 0.75 -0.3 2,895,548 + SO2772 0.75 -0.2 2,895,548 + SO2772 0.75 +0.1 2,895,556 - SO2772 0.77 +0.0 2,895,556 - SO2772 0.77 -0.6 2,895,588 + SO2772 0.83 -0.1 2,895,938 - SO2773 0.42 -0.8 2,895,992 + SO2773 0.56 +0.5 2,896,128 - +0.8 2,896,381 + -1.7 2,896,392 - -1.7 2,896,927 - fabF-1 SO2774 0.48 -2.9 2,896,979 - fabF-1 SO2774 0.52 -3.3 2,897,280 - fabF-1 SO2774 0.77 -1.0 2,897,285 - fabF-1 SO2774 0.77 -1.1 2,897,301 - fabF-1 SO2774 0.78 -1.9 2,897,360 - fabF-1 SO2774 0.83 +0.4 2,897,473 - -0.4 2,897,485 - -2.4 2,897,532 + -1.2 2,897,600 + -1.6 2,898,140 + +0.7 2,898,180 - -0.6 2,898,180 - -1.3
Or see this region's nucleotide sequence