Experiment: Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvB and ruvA are separated by 85 nucleotides ruvA and ruvC overlap by 4 nucleotides ruvC and SO2432 are separated by 188 nucleotides
SO2429: ruvB - Holliday junction DNA helicase RuvB (NCBI ptt file), at 2,538,186 to 2,539,190
ruvB
SO2430: ruvA - Holliday junction DNA helicase RuvA (NCBI ptt file), at 2,539,276 to 2,539,893
ruvA
SO2431: ruvC - crossover junction endodeoxyribonuclease RuvC (NCBI ptt file), at 2,539,890 to 2,540,411
ruvC
SO2432: SO2432 - conserved hypothetical protein TIGR01033 (NCBI ptt file), at 2,540,600 to 2,541,346
SO2432
Position (kb)
2539
2540 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2538.446 kb on + strand, within ruvB at 2538.619 kb on - strand, within ruvB at 2538.695 kb on - strand, within ruvB at 2539.094 kb on - strand at 2539.131 kb on + strand at 2539.233 kb on - strand at 2539.326 kb on - strand at 2539.326 kb on - strand at 2539.405 kb on + strand, within ruvA at 2539.481 kb on - strand, within ruvA at 2539.503 kb on - strand, within ruvA at 2539.584 kb on + strand, within ruvA at 2539.886 kb on + strand at 2539.891 kb on + strand at 2539.964 kb on - strand, within ruvC at 2540.395 kb on - strand at 2540.475 kb on + strand at 2540.483 kb on - strand at 2540.570 kb on - strand at 2540.855 kb on - strand, within SO2432
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.06 mg/ml remove 2,538,446 + ruvB SO2429 0.26 -3.1 2,538,619 - ruvB SO2429 0.43 -2.5 2,538,695 - ruvB SO2429 0.51 -0.3 2,539,094 - -2.0 2,539,131 + +0.9 2,539,233 - +0.3 2,539,326 - -2.4 2,539,326 - -1.9 2,539,405 + ruvA SO2430 0.21 +0.7 2,539,481 - ruvA SO2430 0.33 -0.6 2,539,503 - ruvA SO2430 0.37 -0.7 2,539,584 + ruvA SO2430 0.50 -1.1 2,539,886 + -0.3 2,539,891 + -0.9 2,539,964 - ruvC SO2431 0.14 -3.4 2,540,395 - -1.9 2,540,475 + -0.7 2,540,483 - +0.4 2,540,570 - +1.0 2,540,855 - SO2432 0.34 -0.3
Or see this region's nucleotide sequence