Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0004

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0003 and Psest_0004 are separated by 13 nucleotidesPsest_0004 and Psest_0005 overlap by 4 nucleotides Psest_0003: Psest_0003 - DNA polymerase III, beta subunit, at 2,143 to 3,246 _0003 Psest_0004: Psest_0004 - recF protein, at 3,260 to 4,372 _0004 Psest_0005: Psest_0005 - DNA gyrase, B subunit, at 4,369 to 6,783 _0005 Position (kb) 3 4 5Strain fitness (log2 ratio) -3 -2 -1 0 1at 3.245 kb on - strandat 3.258 kb on + strandat 3.258 kb on + strandat 3.258 kb on + strandat 3.258 kb on + strandat 3.258 kb on + strandat 3.259 kb on - strandat 3.259 kb on - strandat 3.316 kb on - strandat 3.332 kb on - strandat 3.446 kb on + strand, within Psest_0004at 3.446 kb on + strand, within Psest_0004at 3.447 kb on - strand, within Psest_0004at 3.447 kb on - strand, within Psest_0004at 3.447 kb on - strand, within Psest_0004at 3.447 kb on - strand, within Psest_0004at 3.447 kb on - strand, within Psest_0004at 3.447 kb on - strand, within Psest_0004at 3.540 kb on + strand, within Psest_0004at 3.540 kb on + strand, within Psest_0004at 3.540 kb on + strand, within Psest_0004at 3.541 kb on - strand, within Psest_0004at 3.541 kb on - strand, within Psest_0004at 3.541 kb on - strand, within Psest_0004at 3.613 kb on + strand, within Psest_0004at 3.622 kb on + strand, within Psest_0004at 3.622 kb on + strand, within Psest_0004at 3.622 kb on + strand, within Psest_0004at 3.766 kb on + strand, within Psest_0004at 3.766 kb on + strand, within Psest_0004at 3.848 kb on + strand, within Psest_0004at 3.848 kb on + strand, within Psest_0004at 3.848 kb on + strand, within Psest_0004at 3.848 kb on + strand, within Psest_0004at 3.848 kb on + strand, within Psest_0004at 3.848 kb on + strand, within Psest_0004at 3.848 kb on + strand, within Psest_0004at 3.848 kb on + strand, within Psest_0004at 3.849 kb on - strand, within Psest_0004at 3.923 kb on + strand, within Psest_0004at 3.923 kb on + strand, within Psest_0004at 3.923 kb on + strand, within Psest_0004at 4.004 kb on - strand, within Psest_0004at 4.107 kb on + strand, within Psest_0004at 4.107 kb on + strand, within Psest_0004at 4.107 kb on + strand, within Psest_0004at 4.111 kb on + strand, within Psest_0004at 4.111 kb on + strand, within Psest_0004at 4.111 kb on + strand, within Psest_0004at 4.111 kb on + strand, within Psest_0004at 4.111 kb on + strand, within Psest_0004at 4.112 kb on - strand, within Psest_0004at 4.160 kb on + strand, within Psest_0004at 4.160 kb on + strand, within Psest_0004at 4.260 kb on + strand, within Psest_0004

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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3,245 - +0.3
3,258 + -1.0
3,258 + +0.2
3,258 + -1.8
3,258 + -0.1
3,258 + -0.3
3,259 - +0.1
3,259 - -0.6
3,316 - -0.9
3,332 - +0.2
3,446 + Psest_0004 0.17 -0.3
3,446 + Psest_0004 0.17 -1.8
3,447 - Psest_0004 0.17 -0.2
3,447 - Psest_0004 0.17 +0.3
3,447 - Psest_0004 0.17 -1.0
3,447 - Psest_0004 0.17 -1.9
3,447 - Psest_0004 0.17 -1.9
3,447 - Psest_0004 0.17 -1.0
3,540 + Psest_0004 0.25 -1.9
3,540 + Psest_0004 0.25 -1.1
3,540 + Psest_0004 0.25 -0.9
3,541 - Psest_0004 0.25 -1.8
3,541 - Psest_0004 0.25 -1.2
3,541 - Psest_0004 0.25 +0.1
3,613 + Psest_0004 0.32 -0.4
3,622 + Psest_0004 0.33 +0.2
3,622 + Psest_0004 0.33 +0.2
3,622 + Psest_0004 0.33 -1.3
3,766 + Psest_0004 0.45 -0.7
3,766 + Psest_0004 0.45 -1.7
3,848 + Psest_0004 0.53 -0.9
3,848 + Psest_0004 0.53 -1.1
3,848 + Psest_0004 0.53 -0.4
3,848 + Psest_0004 0.53 -0.3
3,848 + Psest_0004 0.53 -0.9
3,848 + Psest_0004 0.53 -0.4
3,848 + Psest_0004 0.53 +0.1
3,848 + Psest_0004 0.53 -0.1
3,849 - Psest_0004 0.53 -3.7
3,923 + Psest_0004 0.60 -0.9
3,923 + Psest_0004 0.60 -0.9
3,923 + Psest_0004 0.60 -0.4
4,004 - Psest_0004 0.67 +1.5
4,107 + Psest_0004 0.76 -2.9
4,107 + Psest_0004 0.76 -0.6
4,107 + Psest_0004 0.76 -0.8
4,111 + Psest_0004 0.76 +1.0
4,111 + Psest_0004 0.76 -1.7
4,111 + Psest_0004 0.76 +0.2
4,111 + Psest_0004 0.76 -1.6
4,111 + Psest_0004 0.76 -0.4
4,112 - Psest_0004 0.77 -1.7
4,160 + Psest_0004 0.81 -0.8
4,160 + Psest_0004 0.81 +0.2
4,260 + Psest_0004 0.90 +1.4

Or see this region's nucleotide sequence