Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1985 and Psest_1986 overlap by 4 nucleotides Psest_1986 and Psest_1987 overlap by 4 nucleotides Psest_1987 and Psest_1988 are separated by 191 nucleotides
Psest_1985: Psest_1985 - histidinol-phosphate aminotransferase, at 2,101,462 to 2,102,571
_1985
Psest_1986: Psest_1986 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 2,102,568 to 2,104,811
_1986
Psest_1987: Psest_1987 - cytidylate kinase, at 2,104,808 to 2,105,497
_1987
Psest_1988: Psest_1988 - ribosomal protein S1, at 2,105,689 to 2,107,371
_1988
Position (kb)
2102
2103
2104
2105 Strain fitness (log2 ratio)
-2
-1
0
1 at 2101.625 kb on + strand, within Psest_1985 at 2101.625 kb on + strand, within Psest_1985 at 2102.059 kb on + strand, within Psest_1985 at 2102.059 kb on + strand, within Psest_1985 at 2102.059 kb on + strand, within Psest_1985 at 2102.059 kb on + strand, within Psest_1985 at 2102.059 kb on + strand, within Psest_1985 at 2102.059 kb on + strand, within Psest_1985 at 2102.059 kb on + strand, within Psest_1985 at 2102.182 kb on + strand, within Psest_1985 at 2102.213 kb on + strand, within Psest_1985 at 2102.247 kb on + strand, within Psest_1985 at 2102.293 kb on + strand, within Psest_1985 at 2103.460 kb on + strand, within Psest_1986 at 2103.477 kb on + strand, within Psest_1986
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,101,625 + Psest_1985 0.15 -0.1 2,101,625 + Psest_1985 0.15 +0.2 2,102,059 + Psest_1985 0.54 +0.2 2,102,059 + Psest_1985 0.54 -0.8 2,102,059 + Psest_1985 0.54 +0.1 2,102,059 + Psest_1985 0.54 +0.0 2,102,059 + Psest_1985 0.54 +0.4 2,102,059 + Psest_1985 0.54 -2.0 2,102,059 + Psest_1985 0.54 +0.8 2,102,182 + Psest_1985 0.65 -0.1 2,102,213 + Psest_1985 0.68 +0.0 2,102,247 + Psest_1985 0.71 -0.3 2,102,293 + Psest_1985 0.75 -0.1 2,103,460 + Psest_1986 0.40 -0.9 2,103,477 + Psest_1986 0.41 -0.4
Or see this region's nucleotide sequence