Experiment: A22 0.0109375 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and thiG overlap by 4 nucleotides thiG and Sama_1857 are separated by 1 nucleotides Sama_1857 and Sama_1858 overlap by 7 nucleotides Sama_1858 and Sama_1859 overlap by 11 nucleotides
Sama_1855: thiH - thiamine biosynthesis protein ThiH (RefSeq), at 2,259,305 to 2,260,420
thiH
Sama_1856: thiG - thiazole synthase (RefSeq), at 2,260,417 to 2,261,232
thiG
Sama_1857: Sama_1857 - thiamine biosynthesis protein ThiS (RefSeq), at 2,261,234 to 2,261,437
_1857
Sama_1858: Sama_1858 - thiF protein, putative (RefSeq), at 2,261,431 to 2,262,183
_1858
Sama_1859: Sama_1859 - phosphomethylpyrimidine kinase/thiamine-phosphate diphosphorylase (RefSeq), at 2,262,173 to 2,263,750
_1859
Position (kb)
2260
2261
2262 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2259.432 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.568 kb on + strand, within thiH at 2259.568 kb on + strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.587 kb on - strand, within thiH at 2259.649 kb on + strand, within thiH at 2259.649 kb on + strand, within thiH at 2259.684 kb on + strand, within thiH at 2259.793 kb on + strand, within thiH at 2259.809 kb on - strand, within thiH at 2259.847 kb on - strand, within thiH at 2259.847 kb on - strand, within thiH at 2259.847 kb on - strand, within thiH at 2259.847 kb on - strand, within thiH at 2259.862 kb on - strand, within thiH at 2259.955 kb on - strand, within thiH at 2259.988 kb on + strand, within thiH at 2260.051 kb on - strand, within thiH at 2260.085 kb on - strand, within thiH at 2260.115 kb on - strand, within thiH at 2260.143 kb on + strand, within thiH at 2260.176 kb on + strand, within thiH at 2260.176 kb on + strand, within thiH at 2260.176 kb on + strand, within thiH at 2260.177 kb on - strand, within thiH at 2260.177 kb on - strand, within thiH at 2260.188 kb on + strand, within thiH at 2260.188 kb on + strand, within thiH at 2260.188 kb on + strand, within thiH at 2260.189 kb on - strand, within thiH at 2260.189 kb on - strand, within thiH at 2260.192 kb on + strand, within thiH at 2260.192 kb on + strand, within thiH at 2260.192 kb on + strand, within thiH at 2260.193 kb on - strand, within thiH at 2260.217 kb on - strand, within thiH at 2260.257 kb on + strand, within thiH at 2260.353 kb on + strand at 2260.372 kb on + strand at 2260.372 kb on + strand at 2260.372 kb on + strand at 2260.408 kb on - strand at 2260.474 kb on - strand at 2260.621 kb on - strand, within thiG at 2260.623 kb on + strand, within thiG at 2260.768 kb on + strand, within thiG at 2260.847 kb on + strand, within thiG at 2260.847 kb on + strand, within thiG at 2260.847 kb on + strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.855 kb on + strand, within thiG at 2260.856 kb on - strand, within thiG at 2260.856 kb on - strand, within thiG at 2260.861 kb on - strand, within thiG at 2260.861 kb on - strand, within thiG at 2260.861 kb on - strand, within thiG at 2260.922 kb on + strand, within thiG at 2260.923 kb on - strand, within thiG at 2261.164 kb on - strand at 2261.281 kb on + strand, within Sama_1857 at 2261.290 kb on + strand, within Sama_1857 at 2261.291 kb on - strand, within Sama_1857 at 2261.291 kb on - strand, within Sama_1857 at 2261.291 kb on - strand, within Sama_1857 at 2261.291 kb on - strand, within Sama_1857 at 2261.302 kb on + strand, within Sama_1857 at 2261.303 kb on - strand, within Sama_1857 at 2261.315 kb on + strand, within Sama_1857 at 2261.315 kb on + strand, within Sama_1857 at 2261.316 kb on - strand, within Sama_1857 at 2261.316 kb on - strand, within Sama_1857 at 2261.316 kb on - strand, within Sama_1857 at 2261.369 kb on - strand, within Sama_1857 at 2261.428 kb on + strand at 2261.623 kb on - strand, within Sama_1858 at 2261.623 kb on - strand, within Sama_1858 at 2261.748 kb on + strand, within Sama_1858 at 2261.749 kb on - strand, within Sama_1858 at 2261.775 kb on - strand, within Sama_1858 at 2261.784 kb on + strand, within Sama_1858 at 2261.848 kb on + strand, within Sama_1858 at 2261.849 kb on - strand, within Sama_1858 at 2261.862 kb on + strand, within Sama_1858 at 2261.863 kb on - strand, within Sama_1858 at 2261.874 kb on - strand, within Sama_1858 at 2261.888 kb on - strand, within Sama_1858 at 2261.888 kb on - strand, within Sama_1858 at 2261.983 kb on - strand, within Sama_1858 at 2262.016 kb on + strand, within Sama_1858 at 2262.016 kb on + strand, within Sama_1858 at 2262.163 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.0109375 mg/ml remove 2,259,432 + thiH Sama_1855 0.11 -0.4 2,259,448 + thiH Sama_1855 0.13 +0.2 2,259,448 + thiH Sama_1855 0.13 -1.3 2,259,448 + thiH Sama_1855 0.13 -1.7 2,259,448 + thiH Sama_1855 0.13 +1.2 2,259,448 + thiH Sama_1855 0.13 -0.9 2,259,449 - thiH Sama_1855 0.13 -0.3 2,259,449 - thiH Sama_1855 0.13 -1.1 2,259,449 - thiH Sama_1855 0.13 -0.8 2,259,449 - thiH Sama_1855 0.13 +1.6 2,259,449 - thiH Sama_1855 0.13 -2.0 2,259,568 + thiH Sama_1855 0.24 -2.5 2,259,568 + thiH Sama_1855 0.24 -0.2 2,259,569 - thiH Sama_1855 0.24 -0.8 2,259,569 - thiH Sama_1855 0.24 +0.2 2,259,569 - thiH Sama_1855 0.24 -0.2 2,259,569 - thiH Sama_1855 0.24 +0.8 2,259,569 - thiH Sama_1855 0.24 +0.6 2,259,587 - thiH Sama_1855 0.25 +0.2 2,259,649 + thiH Sama_1855 0.31 +0.6 2,259,649 + thiH Sama_1855 0.31 +0.0 2,259,684 + thiH Sama_1855 0.34 +1.2 2,259,793 + thiH Sama_1855 0.44 +0.0 2,259,809 - thiH Sama_1855 0.45 +1.0 2,259,847 - thiH Sama_1855 0.49 +0.2 2,259,847 - thiH Sama_1855 0.49 +1.6 2,259,847 - thiH Sama_1855 0.49 -0.6 2,259,847 - thiH Sama_1855 0.49 -0.5 2,259,862 - thiH Sama_1855 0.50 +0.3 2,259,955 - thiH Sama_1855 0.58 +0.9 2,259,988 + thiH Sama_1855 0.61 +0.5 2,260,051 - thiH Sama_1855 0.67 +0.5 2,260,085 - thiH Sama_1855 0.70 +0.0 2,260,115 - thiH Sama_1855 0.73 -1.0 2,260,143 + thiH Sama_1855 0.75 +0.0 2,260,176 + thiH Sama_1855 0.78 +0.3 2,260,176 + thiH Sama_1855 0.78 -0.7 2,260,176 + thiH Sama_1855 0.78 -0.6 2,260,177 - thiH Sama_1855 0.78 -1.3 2,260,177 - thiH Sama_1855 0.78 -0.7 2,260,188 + thiH Sama_1855 0.79 -2.5 2,260,188 + thiH Sama_1855 0.79 +0.2 2,260,188 + thiH Sama_1855 0.79 +0.1 2,260,189 - thiH Sama_1855 0.79 -0.6 2,260,189 - thiH Sama_1855 0.79 +0.1 2,260,192 + thiH Sama_1855 0.79 +0.0 2,260,192 + thiH Sama_1855 0.79 +1.3 2,260,192 + thiH Sama_1855 0.79 -0.8 2,260,193 - thiH Sama_1855 0.80 +1.9 2,260,217 - thiH Sama_1855 0.82 +1.1 2,260,257 + thiH Sama_1855 0.85 -0.1 2,260,353 + -0.5 2,260,372 + +0.0 2,260,372 + -2.6 2,260,372 + -1.1 2,260,408 - -0.2 2,260,474 - -0.4 2,260,621 - thiG Sama_1856 0.25 -0.2 2,260,623 + thiG Sama_1856 0.25 -0.3 2,260,768 + thiG Sama_1856 0.43 +1.2 2,260,847 + thiG Sama_1856 0.53 -1.4 2,260,847 + thiG Sama_1856 0.53 -0.6 2,260,847 + thiG Sama_1856 0.53 -1.0 2,260,848 - thiG Sama_1856 0.53 +0.0 2,260,848 - thiG Sama_1856 0.53 +0.3 2,260,848 - thiG Sama_1856 0.53 -0.3 2,260,848 - thiG Sama_1856 0.53 -0.1 2,260,848 - thiG Sama_1856 0.53 +1.2 2,260,848 - thiG Sama_1856 0.53 +0.6 2,260,848 - thiG Sama_1856 0.53 -0.8 2,260,848 - thiG Sama_1856 0.53 -0.4 2,260,855 + thiG Sama_1856 0.54 -0.1 2,260,856 - thiG Sama_1856 0.54 +0.5 2,260,856 - thiG Sama_1856 0.54 -1.2 2,260,861 - thiG Sama_1856 0.54 -0.8 2,260,861 - thiG Sama_1856 0.54 +0.4 2,260,861 - thiG Sama_1856 0.54 +0.4 2,260,922 + thiG Sama_1856 0.62 +0.0 2,260,923 - thiG Sama_1856 0.62 -0.7 2,261,164 - +1.1 2,261,281 + Sama_1857 0.23 -0.2 2,261,290 + Sama_1857 0.27 -0.4 2,261,291 - Sama_1857 0.28 -0.8 2,261,291 - Sama_1857 0.28 -0.6 2,261,291 - Sama_1857 0.28 +0.3 2,261,291 - Sama_1857 0.28 -0.6 2,261,302 + Sama_1857 0.33 +0.3 2,261,303 - Sama_1857 0.34 -1.3 2,261,315 + Sama_1857 0.40 +0.3 2,261,315 + Sama_1857 0.40 -0.2 2,261,316 - Sama_1857 0.40 +0.0 2,261,316 - Sama_1857 0.40 -1.4 2,261,316 - Sama_1857 0.40 -0.2 2,261,369 - Sama_1857 0.66 -0.7 2,261,428 + -1.1 2,261,623 - Sama_1858 0.25 +1.2 2,261,623 - Sama_1858 0.25 +0.6 2,261,748 + Sama_1858 0.42 +1.3 2,261,749 - Sama_1858 0.42 -0.3 2,261,775 - Sama_1858 0.46 -0.6 2,261,784 + Sama_1858 0.47 +0.5 2,261,848 + Sama_1858 0.55 -1.4 2,261,849 - Sama_1858 0.56 +0.7 2,261,862 + Sama_1858 0.57 +0.4 2,261,863 - Sama_1858 0.57 -1.3 2,261,874 - Sama_1858 0.59 +0.2 2,261,888 - Sama_1858 0.61 +0.3 2,261,888 - Sama_1858 0.61 +1.0 2,261,983 - Sama_1858 0.73 -0.2 2,262,016 + Sama_1858 0.78 -0.2 2,262,016 + Sama_1858 0.78 -0.4 2,262,163 - +0.2
Or see this region's nucleotide sequence