Experiment: Vancomycin 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cheY-3 and fliA are separated by 47 nucleotides fliA and flhG overlap by 8 nucleotides flhG and flhF are separated by 34 nucleotides
SO3209: cheY-3 - chemotaxis protein CheY (NCBI ptt file), at 3,349,724 to 3,350,107
cheY-3
SO3210: fliA - RNA polymerase sigma-27 factor (NCBI ptt file), at 3,350,155 to 3,350,868
fliA
SO3211: flhG - flagellar biosynthetic protein FlhG (NCBI ptt file), at 3,350,861 to 3,351,712
flhG
SO3212: flhF - flagellar biosynthetic protein FlhF (NCBI ptt file), at 3,351,747 to 3,353,123
flhF
Position (kb)
3350
3351
3352 Strain fitness (log2 ratio)
-2
-1
0
1 at 3349.899 kb on + strand, within cheY-3 at 3350.007 kb on + strand, within cheY-3 at 3350.116 kb on - strand at 3350.191 kb on - strand at 3350.534 kb on - strand, within fliA at 3350.671 kb on - strand, within fliA at 3350.686 kb on - strand, within fliA at 3350.731 kb on - strand, within fliA at 3350.732 kb on + strand, within fliA at 3350.845 kb on + strand at 3350.853 kb on - strand at 3350.853 kb on - strand at 3350.853 kb on - strand at 3351.311 kb on - strand, within flhG at 3351.457 kb on - strand, within flhG at 3351.511 kb on - strand, within flhG at 3351.571 kb on - strand, within flhG at 3351.591 kb on + strand, within flhG at 3351.638 kb on + strand at 3351.646 kb on - strand at 3351.770 kb on - strand at 3351.770 kb on - strand at 3351.982 kb on - strand, within flhF at 3352.025 kb on - strand, within flhF at 3352.032 kb on + strand, within flhF at 3352.045 kb on - strand, within flhF at 3352.109 kb on + strand, within flhF at 3352.260 kb on + strand, within flhF at 3352.310 kb on - strand, within flhF at 3352.383 kb on + strand, within flhF at 3352.621 kb on - strand, within flhF at 3352.690 kb on + strand, within flhF
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.03 mg/ml remove 3,349,899 + cheY-3 SO3209 0.46 -0.1 3,350,007 + cheY-3 SO3209 0.74 +0.4 3,350,116 - +1.2 3,350,191 - -0.0 3,350,534 - fliA SO3210 0.53 -1.0 3,350,671 - fliA SO3210 0.72 -0.2 3,350,686 - fliA SO3210 0.74 +0.2 3,350,731 - fliA SO3210 0.81 -2.0 3,350,732 + fliA SO3210 0.81 -0.9 3,350,845 + -0.5 3,350,853 - +0.3 3,350,853 - +0.5 3,350,853 - +0.3 3,351,311 - flhG SO3211 0.53 -1.1 3,351,457 - flhG SO3211 0.70 -1.1 3,351,511 - flhG SO3211 0.76 -1.2 3,351,571 - flhG SO3211 0.83 -1.9 3,351,591 + flhG SO3211 0.86 -0.5 3,351,638 + +1.1 3,351,646 - -1.2 3,351,770 - +0.2 3,351,770 - +0.4 3,351,982 - flhF SO3212 0.17 -0.1 3,352,025 - flhF SO3212 0.20 +0.4 3,352,032 + flhF SO3212 0.21 -1.2 3,352,045 - flhF SO3212 0.22 +1.5 3,352,109 + flhF SO3212 0.26 -0.5 3,352,260 + flhF SO3212 0.37 +0.3 3,352,310 - flhF SO3212 0.41 +0.0 3,352,383 + flhF SO3212 0.46 -0.6 3,352,621 - flhF SO3212 0.63 -1.0 3,352,690 + flhF SO3212 0.68 +0.4
Or see this region's nucleotide sequence