Experiment: Vancomycin 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2614 and pabC overlap by 4 nucleotides pabC and dcd are separated by 5 nucleotides dcd and udk are separated by 29 nucleotides udk and SO2618 are separated by 11 nucleotides
SO2614: SO2614 - conserved hypothetical protein TIGR00247 (NCBI ptt file), at 2,750,354 to 2,751,364
SO2614
SO2615: pabC - 4-amino-4-deoxychorismate lyase (NCBI ptt file), at 2,751,361 to 2,752,170
pabC
SO2616: dcd - deoxycytidine triphosphate deaminase (NCBI ptt file), at 2,752,176 to 2,752,757
dcd
SO2617: udk - uridine kinase (NCBI ptt file), at 2,752,787 to 2,753,425
udk
SO2618: SO2618 - ATP-binding protein, Mrp/Nbp35 family (NCBI ptt file), at 2,753,437 to 2,754,552
SO2618
Position (kb)
2752
2753 Strain fitness (log2 ratio)
-2
-1
0
1 at 2751.419 kb on - strand at 2751.419 kb on - strand at 2751.555 kb on - strand, within pabC at 2751.734 kb on - strand, within pabC at 2751.758 kb on - strand, within pabC at 2751.948 kb on - strand, within pabC at 2752.526 kb on - strand, within dcd at 2753.076 kb on - strand, within udk at 2753.143 kb on + strand, within udk at 2753.194 kb on - strand, within udk at 2753.221 kb on - strand, within udk at 2753.436 kb on - strand at 2753.623 kb on - strand, within SO2618 at 2753.623 kb on - strand, within SO2618 at 2753.744 kb on - strand, within SO2618
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.03 mg/ml remove 2,751,419 - +1.4 2,751,419 - -0.1 2,751,555 - pabC SO2615 0.24 -1.6 2,751,734 - pabC SO2615 0.46 -0.0 2,751,758 - pabC SO2615 0.49 -0.4 2,751,948 - pabC SO2615 0.72 -0.3 2,752,526 - dcd SO2616 0.60 -1.8 2,753,076 - udk SO2617 0.45 +0.1 2,753,143 + udk SO2617 0.56 +1.0 2,753,194 - udk SO2617 0.64 +0.9 2,753,221 - udk SO2617 0.68 -0.6 2,753,436 - -1.0 2,753,623 - SO2618 0.17 -0.7 2,753,623 - SO2618 0.17 -0.7 2,753,744 - SO2618 0.28 -0.1
Or see this region's nucleotide sequence