Experiment: Vancomycin 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2247 and sdaA are separated by 101 nucleotides sdaA and SO2249 overlap by 8 nucleotides SO2249 and nagZ are separated by 41 nucleotides
SO2247: SO2247 - hypothetical protein (NCBI ptt file), at 2,361,634 to 2,362,371
SO2247
SO2248: sdaA - L-serine dehydratase 1 (NCBI ptt file), at 2,362,473 to 2,363,849
sdaA
SO2249: SO2249 - hypothetical protein (NCBI ptt file), at 2,363,842 to 2,364,192
SO2249
SO2250: nagZ - beta-hexosaminidase (NCBI ptt file), at 2,364,234 to 2,365,262
nagZ
Position (kb)
2362
2363
2364 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2361.479 kb on - strand at 2361.479 kb on - strand at 2361.479 kb on - strand at 2361.479 kb on - strand at 2361.502 kb on + strand at 2361.665 kb on + strand at 2361.680 kb on - strand at 2361.854 kb on - strand, within SO2247 at 2361.856 kb on + strand, within SO2247 at 2361.864 kb on - strand, within SO2247 at 2361.875 kb on + strand, within SO2247 at 2362.179 kb on + strand, within SO2247 at 2362.246 kb on - strand, within SO2247 at 2362.322 kb on - strand at 2362.327 kb on - strand at 2362.333 kb on + strand at 2362.471 kb on + strand at 2362.489 kb on - strand at 2362.567 kb on + strand at 2362.577 kb on + strand at 2362.617 kb on - strand, within sdaA at 2363.055 kb on - strand, within sdaA at 2363.055 kb on - strand, within sdaA at 2363.140 kb on - strand, within sdaA at 2363.243 kb on + strand, within sdaA at 2363.373 kb on - strand, within sdaA at 2363.454 kb on - strand, within sdaA at 2363.465 kb on + strand, within sdaA at 2363.681 kb on - strand, within sdaA at 2363.700 kb on + strand, within sdaA at 2363.713 kb on - strand at 2364.270 kb on - strand at 2364.275 kb on - strand at 2364.309 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.03 mg/ml remove 2,361,479 - +0.1 2,361,479 - +0.7 2,361,479 - -0.8 2,361,479 - +1.9 2,361,502 + -0.3 2,361,665 + +0.3 2,361,680 - +0.9 2,361,854 - SO2247 0.30 -0.1 2,361,856 + SO2247 0.30 +1.7 2,361,864 - SO2247 0.31 +0.4 2,361,875 + SO2247 0.33 +0.5 2,362,179 + SO2247 0.74 -0.8 2,362,246 - SO2247 0.83 +0.0 2,362,322 - +0.9 2,362,327 - +1.2 2,362,333 + -0.2 2,362,471 + -0.2 2,362,489 - +0.8 2,362,567 + -1.5 2,362,577 + +1.4 2,362,617 - sdaA SO2248 0.10 -0.9 2,363,055 - sdaA SO2248 0.42 -1.9 2,363,055 - sdaA SO2248 0.42 -0.5 2,363,140 - sdaA SO2248 0.48 -1.3 2,363,243 + sdaA SO2248 0.56 +1.0 2,363,373 - sdaA SO2248 0.65 -0.3 2,363,454 - sdaA SO2248 0.71 -0.6 2,363,465 + sdaA SO2248 0.72 -0.7 2,363,681 - sdaA SO2248 0.88 -2.1 2,363,700 + sdaA SO2248 0.89 +1.8 2,363,713 - -0.8 2,364,270 - -0.2 2,364,275 - +0.1 2,364,309 - +0.3
Or see this region's nucleotide sequence