Experiment: Vancomycin 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2202 and dgkA are separated by 96 nucleotides dgkA and SO2204 are separated by 191 nucleotides SO2204 and SO2205 are separated by 222 nucleotides SO2205 and SO2207 are separated by 280 nucleotides
SO2202: SO2202 - transcriptional regulator, LysR family (NCBI ptt file), at 2,316,537 to 2,317,397
SO2202
SO2203: dgkA - diacylglycerol kinase (NCBI ptt file), at 2,317,494 to 2,317,898
dgkA
SO2204: SO2204 - DNA ligase, ATP-dependent (NCBI ptt file), at 2,318,090 to 2,319,025
SO2204
SO2205: SO2205 - hypothetical protein (NCBI ptt file), at 2,319,248 to 2,319,397
SO2205
SO2207: SO2207 - hypothetical protein (NCBI ptt file), at 2,319,678 to 2,320,091
SO2207
Position (kb)
2318
2319
2320 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2317.107 kb on + strand, within SO2202 at 2317.107 kb on + strand, within SO2202 at 2317.115 kb on + strand, within SO2202 at 2317.130 kb on + strand, within SO2202 at 2317.130 kb on + strand, within SO2202 at 2317.138 kb on - strand, within SO2202 at 2317.138 kb on - strand, within SO2202 at 2317.258 kb on - strand, within SO2202 at 2317.263 kb on - strand, within SO2202 at 2317.300 kb on + strand, within SO2202 at 2317.308 kb on - strand, within SO2202 at 2317.308 kb on - strand, within SO2202 at 2317.308 kb on - strand, within SO2202 at 2317.308 kb on - strand, within SO2202 at 2317.308 kb on - strand, within SO2202 at 2317.515 kb on - strand at 2317.522 kb on + strand at 2317.541 kb on + strand, within dgkA at 2317.549 kb on - strand, within dgkA at 2317.609 kb on - strand, within dgkA at 2317.652 kb on - strand, within dgkA at 2317.713 kb on - strand, within dgkA at 2318.023 kb on + strand at 2318.023 kb on + strand at 2318.023 kb on + strand at 2318.036 kb on - strand at 2318.036 kb on - strand at 2318.095 kb on - strand at 2318.342 kb on - strand, within SO2204 at 2318.365 kb on - strand, within SO2204 at 2318.434 kb on + strand, within SO2204 at 2318.538 kb on + strand, within SO2204 at 2318.540 kb on + strand, within SO2204 at 2318.623 kb on + strand, within SO2204 at 2318.670 kb on + strand, within SO2204 at 2318.678 kb on - strand, within SO2204 at 2318.731 kb on + strand, within SO2204 at 2318.745 kb on - strand, within SO2204 at 2318.802 kb on - strand, within SO2204 at 2318.864 kb on - strand, within SO2204 at 2318.879 kb on + strand, within SO2204 at 2318.879 kb on + strand, within SO2204 at 2318.887 kb on - strand, within SO2204 at 2318.931 kb on + strand, within SO2204 at 2318.989 kb on + strand at 2319.153 kb on + strand at 2319.155 kb on - strand at 2319.221 kb on + strand at 2319.371 kb on + strand, within SO2205 at 2319.437 kb on + strand at 2319.437 kb on + strand at 2319.610 kb on - strand at 2319.750 kb on + strand, within SO2207 at 2319.761 kb on + strand, within SO2207 at 2319.761 kb on + strand, within SO2207 at 2319.836 kb on + strand, within SO2207 at 2319.836 kb on + strand, within SO2207 at 2319.946 kb on + strand, within SO2207
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.03 mg/ml remove 2,317,107 + SO2202 0.66 -1.8 2,317,107 + SO2202 0.66 +0.2 2,317,115 + SO2202 0.67 +1.9 2,317,130 + SO2202 0.69 -0.5 2,317,130 + SO2202 0.69 +0.1 2,317,138 - SO2202 0.70 -0.5 2,317,138 - SO2202 0.70 -0.1 2,317,258 - SO2202 0.84 -0.4 2,317,263 - SO2202 0.84 +0.1 2,317,300 + SO2202 0.89 +0.1 2,317,308 - SO2202 0.90 +0.2 2,317,308 - SO2202 0.90 +0.8 2,317,308 - SO2202 0.90 +0.5 2,317,308 - SO2202 0.90 -1.4 2,317,308 - SO2202 0.90 -0.0 2,317,515 - -0.6 2,317,522 + -0.7 2,317,541 + dgkA SO2203 0.12 +0.7 2,317,549 - dgkA SO2203 0.14 +1.4 2,317,609 - dgkA SO2203 0.28 +0.1 2,317,652 - dgkA SO2203 0.39 +0.3 2,317,713 - dgkA SO2203 0.54 +0.4 2,318,023 + +0.2 2,318,023 + +0.3 2,318,023 + -1.1 2,318,036 - +0.5 2,318,036 - -0.3 2,318,095 - +1.0 2,318,342 - SO2204 0.27 +0.8 2,318,365 - SO2204 0.29 -0.6 2,318,434 + SO2204 0.37 -0.4 2,318,538 + SO2204 0.48 +0.6 2,318,540 + SO2204 0.48 +0.9 2,318,623 + SO2204 0.57 +0.4 2,318,670 + SO2204 0.62 +1.2 2,318,678 - SO2204 0.63 +0.0 2,318,731 + SO2204 0.68 +0.4 2,318,745 - SO2204 0.70 +0.8 2,318,802 - SO2204 0.76 +0.0 2,318,864 - SO2204 0.83 +0.1 2,318,879 + SO2204 0.84 -0.0 2,318,879 + SO2204 0.84 +0.5 2,318,887 - SO2204 0.85 +0.1 2,318,931 + SO2204 0.90 +1.2 2,318,989 + +0.0 2,319,153 + -1.2 2,319,155 - +0.1 2,319,221 + -0.4 2,319,371 + SO2205 0.82 +0.2 2,319,437 + +0.2 2,319,437 + +0.6 2,319,610 - -0.5 2,319,750 + SO2207 0.17 -0.0 2,319,761 + SO2207 0.20 +0.7 2,319,761 + SO2207 0.20 +0.8 2,319,836 + SO2207 0.38 +0.3 2,319,836 + SO2207 0.38 -0.3 2,319,946 + SO2207 0.65 -0.1
Or see this region's nucleotide sequence