Experiment: Vancomycin 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hrpB and mrcB are separated by 77 nucleotides mrcB and ppiC-1 are separated by 77 nucleotides ppiC-1 and SO0636 are separated by 97 nucleotides SO0636 and SO0637 overlap by 4 nucleotides
SO0632: hrpB - ATP-dependent helicase HrpB (NCBI ptt file), at 658,076 to 660,583
hrpB
SO0633: mrcB - penicillin-binding protein 1B (NCBI ptt file), at 660,661 to 662,967
mrcB
SO0635: ppiC-1 - peptidyl-prolyl cis-trans isomerase C (NCBI ptt file), at 663,045 to 663,323
ppiC-1
SO0636: SO0636 - acetyltransferase, GNAT family (NCBI ptt file), at 663,421 to 663,915
SO0636
SO0637: SO0637 - conserved hypothetical protein (NCBI ptt file), at 663,912 to 664,187
SO0637
Position (kb) 660
661
662
663 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 659.697 kb on + strand, within hrpB at 659.705 kb on - strand, within hrpB at 659.729 kb on - strand, within hrpB at 659.776 kb on + strand, within hrpB at 659.841 kb on + strand, within hrpB at 659.841 kb on + strand, within hrpB at 659.849 kb on - strand, within hrpB at 659.849 kb on - strand, within hrpB at 659.849 kb on - strand, within hrpB at 659.851 kb on - strand, within hrpB at 659.859 kb on - strand, within hrpB at 660.060 kb on + strand, within hrpB at 660.068 kb on - strand, within hrpB at 660.068 kb on - strand, within hrpB at 660.104 kb on - strand, within hrpB at 660.249 kb on + strand, within hrpB at 660.249 kb on - strand, within hrpB at 660.273 kb on + strand, within hrpB at 660.281 kb on - strand, within hrpB at 660.289 kb on + strand, within hrpB at 660.336 kb on + strand at 660.336 kb on + strand at 660.376 kb on + strand at 660.383 kb on - strand at 660.395 kb on - strand at 660.402 kb on + strand at 660.405 kb on - strand at 660.457 kb on + strand at 660.495 kb on + strand at 660.533 kb on - strand at 660.584 kb on + strand at 660.584 kb on + strand at 660.584 kb on + strand at 660.592 kb on - strand at 660.592 kb on - strand at 660.592 kb on - strand at 660.619 kb on + strand at 660.627 kb on - strand at 660.746 kb on + strand at 660.746 kb on + strand at 660.754 kb on - strand at 660.793 kb on + strand at 660.799 kb on - strand at 660.884 kb on - strand at 660.917 kb on - strand, within mrcB at 660.972 kb on - strand, within mrcB at 661.037 kb on + strand, within mrcB at 661.078 kb on - strand, within mrcB at 661.274 kb on - strand, within mrcB at 661.324 kb on + strand, within mrcB at 661.332 kb on - strand, within mrcB at 661.334 kb on + strand, within mrcB at 661.342 kb on - strand, within mrcB at 661.488 kb on - strand, within mrcB at 661.629 kb on + strand, within mrcB at 661.629 kb on + strand, within mrcB at 661.637 kb on - strand, within mrcB at 661.641 kb on + strand, within mrcB at 661.646 kb on + strand, within mrcB at 661.646 kb on + strand, within mrcB at 661.646 kb on + strand, within mrcB at 661.646 kb on + strand, within mrcB at 661.646 kb on + strand, within mrcB at 661.646 kb on + strand, within mrcB at 661.646 kb on + strand, within mrcB at 661.646 kb on + strand, within mrcB at 661.654 kb on - strand, within mrcB at 661.654 kb on - strand, within mrcB at 661.695 kb on + strand, within mrcB at 661.695 kb on + strand, within mrcB at 661.701 kb on - strand, within mrcB at 661.703 kb on - strand, within mrcB at 661.703 kb on - strand, within mrcB at 661.703 kb on - strand, within mrcB at 661.710 kb on + strand, within mrcB at 661.731 kb on + strand, within mrcB at 661.731 kb on + strand, within mrcB at 661.739 kb on - strand, within mrcB at 661.823 kb on - strand, within mrcB at 661.823 kb on - strand, within mrcB at 662.041 kb on + strand, within mrcB at 662.185 kb on + strand, within mrcB at 662.234 kb on - strand, within mrcB at 662.324 kb on + strand, within mrcB at 662.410 kb on + strand, within mrcB at 662.436 kb on + strand, within mrcB at 662.465 kb on - strand, within mrcB at 662.475 kb on + strand, within mrcB at 662.565 kb on - strand, within mrcB at 662.611 kb on - strand, within mrcB at 662.663 kb on + strand, within mrcB at 662.673 kb on + strand, within mrcB at 662.803 kb on - strand at 662.868 kb on + strand at 662.903 kb on + strand at 663.090 kb on - strand, within ppiC-1 at 663.200 kb on + strand, within ppiC-1 at 663.432 kb on - strand at 663.475 kb on + strand, within SO0636 at 663.485 kb on - strand, within SO0636 at 663.519 kb on - strand, within SO0636 at 663.588 kb on + strand, within SO0636 at 663.732 kb on + strand, within SO0636 at 663.930 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.03 mg/ml remove 659,697 + hrpB SO0632 0.65 -1.5 659,705 - hrpB SO0632 0.65 +0.4 659,729 - hrpB SO0632 0.66 -0.6 659,776 + hrpB SO0632 0.68 +0.2 659,841 + hrpB SO0632 0.70 -0.3 659,841 + hrpB SO0632 0.70 -0.1 659,849 - hrpB SO0632 0.71 -0.5 659,849 - hrpB SO0632 0.71 +0.0 659,849 - hrpB SO0632 0.71 -0.6 659,851 - hrpB SO0632 0.71 -1.1 659,859 - hrpB SO0632 0.71 +0.1 660,060 + hrpB SO0632 0.79 +0.8 660,068 - hrpB SO0632 0.79 -1.0 660,068 - hrpB SO0632 0.79 +1.0 660,104 - hrpB SO0632 0.81 -0.7 660,249 + hrpB SO0632 0.87 -0.1 660,249 - hrpB SO0632 0.87 -1.0 660,273 + hrpB SO0632 0.88 -0.6 660,281 - hrpB SO0632 0.88 -2.0 660,289 + hrpB SO0632 0.88 -2.0 660,336 + +0.4 660,336 + +1.2 660,376 + -0.4 660,383 - +0.3 660,395 - +0.8 660,402 + +0.2 660,405 - -1.3 660,457 + +0.2 660,495 + +1.0 660,533 - -0.3 660,584 + -0.4 660,584 + -1.3 660,584 + +0.1 660,592 - +0.3 660,592 - -1.6 660,592 - -1.0 660,619 + +0.3 660,627 - -1.0 660,746 + -2.8 660,746 + -1.3 660,754 - -1.6 660,793 + -0.9 660,799 - -1.0 660,884 - -0.3 660,917 - mrcB SO0633 0.11 -1.0 660,972 - mrcB SO0633 0.13 -1.9 661,037 + mrcB SO0633 0.16 -1.5 661,078 - mrcB SO0633 0.18 -1.3 661,274 - mrcB SO0633 0.27 -1.1 661,324 + mrcB SO0633 0.29 -0.3 661,332 - mrcB SO0633 0.29 -3.3 661,334 + mrcB SO0633 0.29 -0.7 661,342 - mrcB SO0633 0.30 -0.7 661,488 - mrcB SO0633 0.36 -0.2 661,629 + mrcB SO0633 0.42 -1.4 661,629 + mrcB SO0633 0.42 -2.3 661,637 - mrcB SO0633 0.42 -1.6 661,641 + mrcB SO0633 0.42 +0.8 661,646 + mrcB SO0633 0.43 -0.7 661,646 + mrcB SO0633 0.43 -1.0 661,646 + mrcB SO0633 0.43 -0.7 661,646 + mrcB SO0633 0.43 -0.9 661,646 + mrcB SO0633 0.43 -0.6 661,646 + mrcB SO0633 0.43 -0.0 661,646 + mrcB SO0633 0.43 -1.8 661,646 + mrcB SO0633 0.43 -2.1 661,654 - mrcB SO0633 0.43 -1.9 661,654 - mrcB SO0633 0.43 -3.5 661,695 + mrcB SO0633 0.45 -0.4 661,695 + mrcB SO0633 0.45 -1.4 661,701 - mrcB SO0633 0.45 -1.1 661,703 - mrcB SO0633 0.45 -1.0 661,703 - mrcB SO0633 0.45 -0.1 661,703 - mrcB SO0633 0.45 -2.8 661,710 + mrcB SO0633 0.45 -1.7 661,731 + mrcB SO0633 0.46 -1.3 661,731 + mrcB SO0633 0.46 -1.5 661,739 - mrcB SO0633 0.47 -0.4 661,823 - mrcB SO0633 0.50 -1.3 661,823 - mrcB SO0633 0.50 -2.0 662,041 + mrcB SO0633 0.60 -1.6 662,185 + mrcB SO0633 0.66 -0.9 662,234 - mrcB SO0633 0.68 -1.7 662,324 + mrcB SO0633 0.72 -0.7 662,410 + mrcB SO0633 0.76 -1.6 662,436 + mrcB SO0633 0.77 -0.8 662,465 - mrcB SO0633 0.78 -1.1 662,475 + mrcB SO0633 0.79 -0.3 662,565 - mrcB SO0633 0.83 -1.4 662,611 - mrcB SO0633 0.85 -1.5 662,663 + mrcB SO0633 0.87 -2.4 662,673 + mrcB SO0633 0.87 -0.7 662,803 - +0.8 662,868 + +0.1 662,903 + -0.7 663,090 - ppiC-1 SO0635 0.16 -0.1 663,200 + ppiC-1 SO0635 0.56 +0.3 663,432 - -0.5 663,475 + SO0636 0.11 +0.1 663,485 - SO0636 0.13 +1.3 663,519 - SO0636 0.20 +0.4 663,588 + SO0636 0.34 -0.3 663,732 + SO0636 0.63 +0.5 663,930 + +0.1
Or see this region's nucleotide sequence