Experiment: Vancomycin 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frdA and frdB overlap by 1 nucleotides frdB and SO0400 are separated by 63 nucleotides SO0400 and SO0401 overlap by 4 nucleotides
SO0398: frdA - fumarate reductase flavoprotein subunit (NCBI ptt file), at 411,381 to 413,387
frdA
SO0399: frdB - fumarate reductase iron-sulfur protein (NCBI ptt file), at 413,387 to 414,118
frdB
SO0400: SO0400 - conserved hypothetical protein (NCBI ptt file), at 414,182 to 414,466
SO0400
SO0401: SO0401 - alcohol dehydrogenase, zinc-containing (NCBI ptt file), at 414,463 to 415,476
SO0401
Position (kb) 413
414
415 Strain fitness (log2 ratio) -2
-1
0
1
2 at 412.465 kb on - strand, within frdA at 412.474 kb on + strand, within frdA at 412.482 kb on - strand, within frdA at 412.535 kb on + strand, within frdA at 412.535 kb on + strand, within frdA at 412.543 kb on - strand, within frdA at 412.543 kb on - strand, within frdA at 412.543 kb on - strand, within frdA at 412.556 kb on + strand, within frdA at 412.599 kb on + strand, within frdA at 412.600 kb on - strand, within frdA at 412.690 kb on + strand, within frdA at 412.698 kb on - strand, within frdA at 412.862 kb on + strand, within frdA at 412.870 kb on - strand, within frdA at 412.870 kb on - strand, within frdA at 412.870 kb on - strand, within frdA at 412.870 kb on - strand, within frdA at 412.870 kb on - strand, within frdA at 412.969 kb on - strand, within frdA at 412.969 kb on - strand, within frdA at 413.196 kb on - strand at 413.206 kb on - strand at 413.301 kb on - strand at 413.313 kb on - strand at 413.331 kb on + strand at 413.397 kb on + strand at 413.460 kb on - strand at 413.573 kb on - strand, within frdB at 413.669 kb on - strand, within frdB at 413.750 kb on - strand, within frdB at 413.798 kb on - strand, within frdB at 413.845 kb on - strand, within frdB at 413.855 kb on - strand, within frdB at 413.861 kb on - strand, within frdB at 413.902 kb on - strand, within frdB at 414.008 kb on + strand, within frdB at 414.016 kb on - strand, within frdB at 414.056 kb on + strand at 414.056 kb on + strand at 414.064 kb on - strand at 414.178 kb on + strand at 414.204 kb on + strand at 414.246 kb on + strand, within SO0400 at 414.261 kb on - strand, within SO0400 at 414.317 kb on - strand, within SO0400 at 414.372 kb on + strand, within SO0400 at 414.412 kb on + strand, within SO0400 at 414.419 kb on + strand, within SO0400 at 414.509 kb on - strand at 414.565 kb on - strand, within SO0401 at 414.636 kb on - strand, within SO0401 at 414.668 kb on - strand, within SO0401 at 414.679 kb on - strand, within SO0401 at 414.681 kb on - strand, within SO0401 at 414.728 kb on - strand, within SO0401 at 414.848 kb on + strand, within SO0401 at 414.848 kb on + strand, within SO0401 at 414.856 kb on - strand, within SO0401 at 414.894 kb on + strand, within SO0401 at 414.910 kb on - strand, within SO0401 at 414.910 kb on - strand, within SO0401 at 414.954 kb on + strand, within SO0401 at 414.954 kb on + strand, within SO0401 at 414.954 kb on + strand, within SO0401 at 414.954 kb on + strand, within SO0401 at 414.962 kb on - strand, within SO0401 at 414.962 kb on - strand, within SO0401 at 414.962 kb on - strand, within SO0401 at 415.092 kb on + strand, within SO0401 at 415.092 kb on + strand, within SO0401 at 415.100 kb on - strand, within SO0401 at 415.102 kb on + strand, within SO0401 at 415.116 kb on - strand, within SO0401
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.03 mg/ml remove 412,465 - frdA SO0398 0.54 +0.3 412,474 + frdA SO0398 0.54 -0.2 412,482 - frdA SO0398 0.55 -0.0 412,535 + frdA SO0398 0.57 -0.3 412,535 + frdA SO0398 0.57 -0.7 412,543 - frdA SO0398 0.58 +1.1 412,543 - frdA SO0398 0.58 -1.2 412,543 - frdA SO0398 0.58 -0.3 412,556 + frdA SO0398 0.59 +0.2 412,599 + frdA SO0398 0.61 +0.0 412,600 - frdA SO0398 0.61 -0.9 412,690 + frdA SO0398 0.65 -0.5 412,698 - frdA SO0398 0.66 +2.0 412,862 + frdA SO0398 0.74 +0.5 412,870 - frdA SO0398 0.74 -0.5 412,870 - frdA SO0398 0.74 +0.0 412,870 - frdA SO0398 0.74 +0.3 412,870 - frdA SO0398 0.74 +0.5 412,870 - frdA SO0398 0.74 -2.5 412,969 - frdA SO0398 0.79 -0.1 412,969 - frdA SO0398 0.79 -0.3 413,196 - +0.0 413,206 - -0.0 413,301 - -0.5 413,313 - +0.1 413,331 + -0.2 413,397 + -0.3 413,460 - -0.3 413,573 - frdB SO0399 0.25 -1.1 413,669 - frdB SO0399 0.39 -0.4 413,750 - frdB SO0399 0.50 -0.8 413,798 - frdB SO0399 0.56 -0.3 413,845 - frdB SO0399 0.63 -0.5 413,855 - frdB SO0399 0.64 +0.0 413,861 - frdB SO0399 0.65 +0.3 413,902 - frdB SO0399 0.70 -0.0 414,008 + frdB SO0399 0.85 -0.3 414,016 - frdB SO0399 0.86 -1.0 414,056 + +0.3 414,056 + +0.4 414,064 - +0.2 414,178 + +0.3 414,204 + -0.2 414,246 + SO0400 0.22 -0.6 414,261 - SO0400 0.28 +1.4 414,317 - SO0400 0.47 -1.4 414,372 + SO0400 0.67 +0.2 414,412 + SO0400 0.81 +0.3 414,419 + SO0400 0.83 -0.7 414,509 - +0.9 414,565 - SO0401 0.10 +0.1 414,636 - SO0401 0.17 -1.5 414,668 - SO0401 0.20 +0.3 414,679 - SO0401 0.21 +0.6 414,681 - SO0401 0.21 -0.2 414,728 - SO0401 0.26 -0.2 414,848 + SO0401 0.38 -0.9 414,848 + SO0401 0.38 +0.8 414,856 - SO0401 0.39 +1.5 414,894 + SO0401 0.43 +0.3 414,910 - SO0401 0.44 +0.1 414,910 - SO0401 0.44 -1.4 414,954 + SO0401 0.48 +0.1 414,954 + SO0401 0.48 +0.3 414,954 + SO0401 0.48 -0.3 414,954 + SO0401 0.48 +0.3 414,962 - SO0401 0.49 +0.6 414,962 - SO0401 0.49 +0.1 414,962 - SO0401 0.49 +0.4 415,092 + SO0401 0.62 -0.0 415,092 + SO0401 0.62 +0.3 415,100 - SO0401 0.63 +0.1 415,102 + SO0401 0.63 +0.6 415,116 - SO0401 0.64 +0.1
Or see this region's nucleotide sequence