Experiment: Vancomycin 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspI and gspJ overlap by 20 nucleotides gspJ and gspK overlap by 4 nucleotides gspK and gspL are separated by 48 nucleotides
SO0171: gspI - general secretion pathway protein I (NCBI ptt file), at 178,274 to 178,642
gspI
SO0172: gspJ - general secretion pathway protein J (NCBI ptt file), at 178,623 to 179,390
gspJ
SO0173: gspK - general secretion pathway protein K (NCBI ptt file), at 179,387 to 180,385
gspK
SO0174: gspL - general secretion pathway protein L (NCBI ptt file), at 180,434 to 181,621
gspL
Position (kb) 179
180
181 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 178.440 kb on + strand, within gspI at 178.516 kb on - strand, within gspI at 178.605 kb on - strand, within gspI at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.662 kb on - strand at 178.662 kb on - strand at 178.700 kb on + strand, within gspJ at 178.730 kb on - strand, within gspJ at 178.742 kb on - strand, within gspJ at 178.744 kb on + strand, within gspJ at 178.816 kb on + strand, within gspJ at 178.824 kb on - strand, within gspJ at 178.849 kb on - strand, within gspJ at 178.850 kb on + strand, within gspJ at 178.867 kb on + strand, within gspJ at 178.943 kb on + strand, within gspJ at 178.943 kb on + strand, within gspJ at 178.950 kb on - strand, within gspJ at 178.951 kb on - strand, within gspJ at 179.018 kb on - strand, within gspJ at 179.151 kb on + strand, within gspJ at 179.157 kb on + strand, within gspJ at 179.220 kb on - strand, within gspJ at 179.273 kb on + strand, within gspJ at 179.303 kb on - strand, within gspJ at 179.394 kb on + strand at 179.436 kb on - strand at 179.544 kb on - strand, within gspK at 179.635 kb on + strand, within gspK at 179.645 kb on + strand, within gspK at 179.785 kb on + strand, within gspK at 179.849 kb on - strand, within gspK at 179.892 kb on + strand, within gspK at 180.055 kb on + strand, within gspK at 180.102 kb on - strand, within gspK at 180.209 kb on - strand, within gspK at 180.410 kb on + strand at 180.418 kb on - strand at 180.424 kb on + strand at 180.476 kb on - strand at 180.493 kb on + strand at 180.538 kb on + strand at 180.546 kb on - strand at 180.546 kb on - strand at 180.569 kb on - strand, within gspL at 180.569 kb on - strand, within gspL at 180.569 kb on - strand, within gspL at 180.628 kb on - strand, within gspL at 180.628 kb on - strand, within gspL at 180.697 kb on - strand, within gspL at 180.910 kb on - strand, within gspL at 180.920 kb on + strand, within gspL at 180.932 kb on + strand, within gspL at 181.035 kb on + strand, within gspL at 181.078 kb on - strand, within gspL at 181.147 kb on - strand, within gspL at 181.225 kb on - strand, within gspL at 181.281 kb on - strand, within gspL at 181.311 kb on - strand, within gspL at 181.364 kb on + strand, within gspL at 181.364 kb on + strand, within gspL at 181.372 kb on - strand, within gspL at 181.372 kb on - strand, within gspL
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.03 mg/ml remove 178,440 + gspI SO0171 0.45 -1.1 178,516 - gspI SO0171 0.66 -1.1 178,605 - gspI SO0171 0.90 -0.2 178,654 + -1.5 178,654 + -2.3 178,654 + -0.7 178,654 + +1.2 178,654 + -2.3 178,654 + -3.1 178,662 - -1.1 178,662 - -1.7 178,700 + gspJ SO0172 0.10 -0.5 178,730 - gspJ SO0172 0.14 -0.9 178,742 - gspJ SO0172 0.15 -1.6 178,744 + gspJ SO0172 0.16 -1.4 178,816 + gspJ SO0172 0.25 +0.6 178,824 - gspJ SO0172 0.26 -2.1 178,849 - gspJ SO0172 0.29 -0.6 178,850 + gspJ SO0172 0.30 -2.4 178,867 + gspJ SO0172 0.32 -0.3 178,943 + gspJ SO0172 0.42 -0.7 178,943 + gspJ SO0172 0.42 -1.2 178,950 - gspJ SO0172 0.43 -0.4 178,951 - gspJ SO0172 0.43 -1.4 179,018 - gspJ SO0172 0.51 -2.6 179,151 + gspJ SO0172 0.69 -2.1 179,157 + gspJ SO0172 0.70 -0.7 179,220 - gspJ SO0172 0.78 -1.6 179,273 + gspJ SO0172 0.85 +0.4 179,303 - gspJ SO0172 0.89 +0.4 179,394 + -1.5 179,436 - -0.3 179,544 - gspK SO0173 0.16 -0.6 179,635 + gspK SO0173 0.25 -0.7 179,645 + gspK SO0173 0.26 -0.8 179,785 + gspK SO0173 0.40 -1.0 179,849 - gspK SO0173 0.46 -1.2 179,892 + gspK SO0173 0.51 -1.7 180,055 + gspK SO0173 0.67 -1.4 180,102 - gspK SO0173 0.72 -1.8 180,209 - gspK SO0173 0.82 -1.7 180,410 + -0.7 180,418 - -1.4 180,424 + +0.2 180,476 - -1.6 180,493 + -1.5 180,538 + -1.9 180,546 - -2.3 180,546 - -0.9 180,569 - gspL SO0174 0.11 -1.6 180,569 - gspL SO0174 0.11 -0.1 180,569 - gspL SO0174 0.11 -0.1 180,628 - gspL SO0174 0.16 -2.1 180,628 - gspL SO0174 0.16 -1.1 180,697 - gspL SO0174 0.22 +1.3 180,910 - gspL SO0174 0.40 -2.8 180,920 + gspL SO0174 0.41 +0.7 180,932 + gspL SO0174 0.42 +0.9 181,035 + gspL SO0174 0.51 -1.0 181,078 - gspL SO0174 0.54 -1.7 181,147 - gspL SO0174 0.60 -2.0 181,225 - gspL SO0174 0.67 -0.5 181,281 - gspL SO0174 0.71 -0.8 181,311 - gspL SO0174 0.74 -0.3 181,364 + gspL SO0174 0.78 -0.0 181,364 + gspL SO0174 0.78 +1.3 181,372 - gspL SO0174 0.79 -1.3 181,372 - gspL SO0174 0.79 +1.3
Or see this region's nucleotide sequence