Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_3519

Experiment: 5-Hydroxymethylfurfural 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_3517 and Psest_3518 are separated by 241 nucleotidesPsest_3518 and Psest_3519 are separated by 236 nucleotidesPsest_3519 and Psest_3520 are separated by 111 nucleotides Psest_3517: Psest_3517 - branched-chain ketoacid ferredoxin reductase (EC 1.2.7.7) active on 4-methyl-2-oxopentanoate, (S)-3-methyl-2-oxopentanoate, or 3-methyl-2-oxobutanoate (from data), at 3,694,196 to 3,697,666 _3517 Psest_3518: Psest_3518 - Transcriptional regulators, at 3,697,908 to 3,698,378 _3518 Psest_3519: Psest_3519 - Uncharacterized conserved protein, at 3,698,615 to 3,699,031 _3519 Psest_3520: Psest_3520 - Short chain fatty acids transporter, at 3,699,143 to 3,700,483 _3520 Position (kb) 3698 3699 3700Strain fitness (log2 ratio) -1 0 1 2at 3697.632 kb on + strandat 3697.678 kb on - strandat 3697.678 kb on - strandat 3697.678 kb on - strandat 3697.852 kb on - strandat 3697.925 kb on - strandat 3698.268 kb on + strand, within Psest_3518at 3698.269 kb on - strand, within Psest_3518at 3698.376 kb on + strandat 3698.377 kb on - strandat 3698.434 kb on - strandat 3698.477 kb on - strandat 3698.616 kb on + strandat 3698.616 kb on + strandat 3698.723 kb on + strand, within Psest_3519at 3698.723 kb on + strand, within Psest_3519at 3698.724 kb on - strand, within Psest_3519at 3698.724 kb on - strand, within Psest_3519at 3699.088 kb on - strandat 3699.117 kb on + strandat 3699.117 kb on + strandat 3699.118 kb on - strandat 3699.118 kb on - strandat 3699.118 kb on - strandat 3699.118 kb on - strandat 3699.173 kb on + strandat 3699.572 kb on + strand, within Psest_3520at 3699.713 kb on + strand, within Psest_3520at 3699.713 kb on + strand, within Psest_3520at 3699.713 kb on + strand, within Psest_3520at 3699.713 kb on + strand, within Psest_3520at 3699.713 kb on + strand, within Psest_3520at 3699.713 kb on + strand, within Psest_3520at 3699.714 kb on - strand, within Psest_3520at 3699.714 kb on - strand, within Psest_3520at 3699.714 kb on - strand, within Psest_3520at 3699.714 kb on - strand, within Psest_3520at 3700.017 kb on - strand, within Psest_3520

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 5 mM
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3,697,632 + -1.0
3,697,678 - -0.6
3,697,678 - -0.6
3,697,678 - +0.3
3,697,852 - -0.3
3,697,925 - -0.4
3,698,268 + Psest_3518 0.76 +0.4
3,698,269 - Psest_3518 0.77 +1.5
3,698,376 + -0.0
3,698,377 - +2.2
3,698,434 - -0.0
3,698,477 - -1.1
3,698,616 + -0.6
3,698,616 + +0.1
3,698,723 + Psest_3519 0.26 +1.2
3,698,723 + Psest_3519 0.26 -0.2
3,698,724 - Psest_3519 0.26 +2.0
3,698,724 - Psest_3519 0.26 -0.2
3,699,088 - -0.0
3,699,117 + +0.8
3,699,117 + +0.4
3,699,118 - +0.8
3,699,118 - -0.6
3,699,118 - -0.5
3,699,118 - +0.5
3,699,173 + +1.3
3,699,572 + Psest_3520 0.32 +1.6
3,699,713 + Psest_3520 0.43 -0.0
3,699,713 + Psest_3520 0.43 +0.4
3,699,713 + Psest_3520 0.43 -0.7
3,699,713 + Psest_3520 0.43 +0.6
3,699,713 + Psest_3520 0.43 +0.5
3,699,713 + Psest_3520 0.43 +1.0
3,699,714 - Psest_3520 0.43 -0.8
3,699,714 - Psest_3520 0.43 +0.2
3,699,714 - Psest_3520 0.43 -0.9
3,699,714 - Psest_3520 0.43 +0.1
3,700,017 - Psest_3520 0.65 +0.4

Or see this region's nucleotide sequence