Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0590 and Sama_0591 are separated by 223 nucleotides Sama_0591 and Sama_0592 are separated by 235 nucleotides
Sama_0590: Sama_0590 - putative TonB-dependent receptor protein (RefSeq), at 736,305 to 738,518
_0590
Sama_0591: Sama_0591 - hypothetical protein (RefSeq), at 738,742 to 739,443
_0591
Sama_0592: Sama_0592 - smc-like chromosome partitioning protein (DUF3584) <small>(from data)</small>, at 739,679 to 743,359
_0592
Position (kb) 738
739
740 Strain fitness (log2 ratio) -2
-1
0
1
2 at 737.764 kb on + strand, within Sama_0590 at 737.764 kb on + strand, within Sama_0590 at 737.764 kb on + strand, within Sama_0590 at 737.765 kb on - strand, within Sama_0590 at 737.778 kb on - strand, within Sama_0590 at 737.872 kb on + strand, within Sama_0590 at 737.878 kb on + strand, within Sama_0590 at 737.878 kb on + strand, within Sama_0590 at 737.878 kb on + strand, within Sama_0590 at 737.879 kb on - strand, within Sama_0590 at 737.879 kb on - strand, within Sama_0590 at 737.879 kb on - strand, within Sama_0590 at 737.879 kb on - strand, within Sama_0590 at 737.879 kb on - strand, within Sama_0590 at 737.941 kb on + strand, within Sama_0590 at 737.942 kb on - strand, within Sama_0590 at 738.053 kb on + strand, within Sama_0590 at 738.053 kb on + strand, within Sama_0590 at 738.053 kb on + strand, within Sama_0590 at 738.054 kb on - strand, within Sama_0590 at 738.054 kb on - strand, within Sama_0590 at 738.066 kb on + strand, within Sama_0590 at 738.066 kb on + strand, within Sama_0590 at 738.067 kb on - strand, within Sama_0590 at 738.067 kb on - strand, within Sama_0590 at 738.106 kb on + strand, within Sama_0590 at 738.106 kb on + strand, within Sama_0590 at 738.106 kb on + strand, within Sama_0590 at 738.106 kb on + strand, within Sama_0590 at 738.107 kb on - strand, within Sama_0590 at 738.107 kb on - strand, within Sama_0590 at 738.107 kb on - strand, within Sama_0590 at 738.107 kb on - strand, within Sama_0590 at 738.107 kb on - strand, within Sama_0590 at 738.109 kb on + strand, within Sama_0590 at 738.109 kb on + strand, within Sama_0590 at 738.110 kb on - strand, within Sama_0590 at 738.110 kb on - strand, within Sama_0590 at 738.127 kb on + strand, within Sama_0590 at 738.127 kb on + strand, within Sama_0590 at 738.128 kb on - strand, within Sama_0590 at 738.128 kb on - strand, within Sama_0590 at 738.128 kb on - strand, within Sama_0590 at 738.128 kb on - strand, within Sama_0590 at 738.226 kb on + strand, within Sama_0590 at 738.226 kb on + strand, within Sama_0590 at 738.227 kb on - strand, within Sama_0590 at 738.245 kb on + strand, within Sama_0590 at 738.245 kb on + strand, within Sama_0590 at 738.246 kb on - strand, within Sama_0590 at 738.246 kb on - strand, within Sama_0590 at 738.246 kb on - strand, within Sama_0590 at 738.246 kb on - strand, within Sama_0590 at 738.247 kb on + strand, within Sama_0590 at 738.247 kb on + strand, within Sama_0590 at 738.247 kb on + strand, within Sama_0590 at 738.247 kb on + strand, within Sama_0590 at 738.247 kb on + strand, within Sama_0590 at 738.248 kb on - strand, within Sama_0590 at 738.248 kb on - strand, within Sama_0590 at 738.248 kb on - strand, within Sama_0590 at 738.248 kb on - strand, within Sama_0590 at 738.248 kb on - strand, within Sama_0590 at 738.248 kb on - strand, within Sama_0590 at 738.289 kb on + strand, within Sama_0590 at 738.289 kb on + strand, within Sama_0590 at 738.289 kb on + strand, within Sama_0590 at 738.290 kb on - strand, within Sama_0590 at 738.290 kb on - strand, within Sama_0590 at 738.290 kb on - strand, within Sama_0590 at 738.299 kb on + strand at 738.299 kb on + strand at 738.299 kb on + strand at 738.300 kb on - strand at 738.509 kb on + strand at 738.510 kb on - strand at 738.548 kb on - strand at 738.553 kb on + strand at 738.554 kb on - strand at 738.558 kb on + strand at 738.574 kb on + strand at 738.593 kb on + strand at 738.617 kb on + strand at 738.618 kb on - strand at 738.644 kb on + strand at 738.669 kb on + strand at 738.669 kb on + strand at 738.669 kb on + strand at 738.669 kb on + strand at 738.669 kb on + strand at 738.669 kb on + strand at 738.669 kb on + strand at 738.670 kb on - strand at 738.670 kb on - strand at 738.670 kb on - strand at 738.678 kb on + strand at 738.679 kb on - strand at 738.687 kb on + strand at 738.687 kb on + strand at 738.731 kb on + strand at 738.741 kb on + strand at 738.741 kb on + strand at 738.843 kb on + strand, within Sama_0591 at 738.843 kb on + strand, within Sama_0591 at 738.913 kb on + strand, within Sama_0591 at 738.913 kb on + strand, within Sama_0591 at 738.913 kb on + strand, within Sama_0591 at 738.913 kb on + strand, within Sama_0591 at 738.914 kb on - strand, within Sama_0591 at 738.914 kb on - strand, within Sama_0591 at 738.914 kb on - strand, within Sama_0591 at 738.957 kb on - strand, within Sama_0591 at 739.026 kb on + strand, within Sama_0591 at 739.033 kb on + strand, within Sama_0591 at 739.033 kb on + strand, within Sama_0591 at 739.034 kb on - strand, within Sama_0591 at 739.034 kb on - strand, within Sama_0591 at 739.034 kb on - strand, within Sama_0591 at 739.061 kb on + strand, within Sama_0591 at 739.061 kb on + strand, within Sama_0591 at 739.061 kb on + strand, within Sama_0591 at 739.062 kb on - strand, within Sama_0591 at 739.062 kb on - strand, within Sama_0591 at 739.062 kb on - strand, within Sama_0591 at 739.102 kb on + strand, within Sama_0591 at 739.102 kb on + strand, within Sama_0591 at 739.102 kb on + strand, within Sama_0591 at 739.102 kb on + strand, within Sama_0591 at 739.102 kb on + strand, within Sama_0591 at 739.102 kb on + strand, within Sama_0591 at 739.102 kb on + strand, within Sama_0591 at 739.102 kb on + strand, within Sama_0591 at 739.102 kb on + strand, within Sama_0591 at 739.102 kb on + strand, within Sama_0591 at 739.102 kb on + strand, within Sama_0591 at 739.102 kb on + strand, within Sama_0591 at 739.103 kb on - strand, within Sama_0591 at 739.103 kb on - strand, within Sama_0591 at 739.103 kb on - strand, within Sama_0591 at 739.103 kb on - strand, within Sama_0591 at 739.103 kb on - strand, within Sama_0591 at 739.103 kb on - strand, within Sama_0591 at 739.103 kb on - strand, within Sama_0591 at 739.103 kb on - strand, within Sama_0591 at 739.103 kb on - strand, within Sama_0591 at 739.103 kb on - strand, within Sama_0591 at 739.122 kb on + strand, within Sama_0591 at 739.123 kb on - strand, within Sama_0591 at 739.123 kb on - strand, within Sama_0591 at 739.123 kb on - strand, within Sama_0591 at 739.128 kb on + strand, within Sama_0591 at 739.129 kb on - strand, within Sama_0591 at 739.129 kb on - strand, within Sama_0591 at 739.129 kb on - strand, within Sama_0591 at 739.173 kb on + strand, within Sama_0591 at 739.198 kb on + strand, within Sama_0591 at 739.198 kb on + strand, within Sama_0591 at 739.199 kb on - strand, within Sama_0591 at 739.199 kb on - strand, within Sama_0591 at 739.206 kb on + strand, within Sama_0591 at 739.318 kb on - strand, within Sama_0591 at 739.329 kb on + strand, within Sama_0591 at 739.329 kb on + strand, within Sama_0591 at 739.329 kb on + strand, within Sama_0591 at 739.329 kb on + strand, within Sama_0591 at 739.330 kb on - strand, within Sama_0591 at 739.330 kb on - strand, within Sama_0591 at 739.330 kb on - strand, within Sama_0591 at 739.330 kb on - strand, within Sama_0591 at 739.330 kb on - strand, within Sama_0591 at 739.330 kb on - strand, within Sama_0591 at 739.351 kb on - strand, within Sama_0591 at 739.404 kb on + strand at 739.417 kb on + strand at 739.417 kb on + strand at 739.417 kb on + strand at 739.441 kb on + strand at 739.459 kb on - strand at 739.490 kb on + strand at 739.491 kb on - strand at 739.541 kb on + strand at 739.546 kb on + strand at 739.546 kb on + strand at 739.546 kb on + strand at 739.546 kb on + strand at 739.547 kb on - strand at 739.547 kb on - strand at 739.549 kb on + strand at 739.549 kb on + strand at 739.549 kb on + strand at 739.549 kb on + strand at 739.550 kb on - strand at 739.550 kb on - strand at 739.550 kb on - strand at 739.550 kb on - strand at 739.550 kb on - strand at 739.550 kb on - strand at 739.550 kb on - strand at 739.550 kb on - strand at 739.583 kb on + strand at 739.583 kb on + strand at 739.583 kb on + strand at 739.583 kb on + strand at 739.583 kb on + strand at 739.583 kb on + strand at 739.584 kb on - strand at 739.584 kb on - strand at 739.584 kb on - strand at 739.584 kb on - strand at 739.584 kb on - strand at 739.584 kb on - strand at 739.587 kb on + strand at 739.587 kb on + strand at 739.588 kb on - strand at 739.588 kb on - strand at 739.588 kb on - strand at 739.593 kb on + strand at 739.594 kb on - strand at 739.603 kb on + strand at 739.603 kb on + strand at 739.604 kb on - strand at 739.604 kb on - strand at 739.604 kb on - strand at 739.668 kb on + strand at 739.674 kb on + strand at 739.675 kb on - strand at 739.684 kb on + strand at 739.684 kb on + strand at 739.684 kb on + strand at 739.685 kb on - strand at 739.685 kb on - strand at 739.685 kb on - strand at 739.754 kb on - strand at 739.759 kb on + strand at 739.759 kb on + strand at 739.759 kb on + strand at 739.759 kb on + strand at 739.759 kb on + strand at 739.760 kb on - strand at 739.827 kb on - strand at 739.875 kb on - strand at 739.895 kb on + strand at 739.895 kb on + strand at 739.895 kb on + strand at 739.895 kb on + strand at 739.895 kb on + strand at 739.895 kb on + strand at 739.895 kb on + strand at 739.896 kb on - strand at 739.896 kb on - strand at 739.896 kb on - strand at 739.896 kb on - strand at 739.896 kb on - strand at 739.896 kb on - strand at 739.897 kb on + strand at 739.898 kb on - strand at 739.904 kb on + strand at 739.905 kb on - strand at 739.934 kb on + strand at 739.935 kb on - strand at 739.947 kb on + strand at 739.947 kb on + strand at 739.947 kb on + strand at 739.947 kb on + strand at 739.947 kb on + strand at 739.972 kb on + strand at 739.972 kb on + strand at 740.015 kb on + strand at 740.016 kb on - strand at 740.016 kb on - strand at 740.016 kb on - strand at 740.016 kb on - strand at 740.137 kb on + strand, within Sama_0592 at 740.138 kb on - strand, within Sama_0592 at 740.188 kb on + strand, within Sama_0592 at 740.188 kb on + strand, within Sama_0592 at 740.188 kb on + strand, within Sama_0592 at 740.189 kb on - strand, within Sama_0592 at 740.195 kb on + strand, within Sama_0592 at 740.195 kb on + strand, within Sama_0592 at 740.195 kb on + strand, within Sama_0592 at 740.195 kb on + strand, within Sama_0592 at 740.195 kb on + strand, within Sama_0592 at 740.195 kb on + strand, within Sama_0592 at 740.196 kb on - strand, within Sama_0592 at 740.196 kb on - strand, within Sama_0592 at 740.235 kb on + strand, within Sama_0592 at 740.235 kb on + strand, within Sama_0592 at 740.236 kb on - strand, within Sama_0592 at 740.334 kb on + strand, within Sama_0592 at 740.356 kb on + strand, within Sama_0592 at 740.398 kb on + strand, within Sama_0592 at 740.398 kb on + strand, within Sama_0592 at 740.398 kb on + strand, within Sama_0592 at 740.398 kb on + strand, within Sama_0592
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 737,764 + Sama_0590 0.66 +0.6 737,764 + Sama_0590 0.66 -2.1 737,764 + Sama_0590 0.66 +0.4 737,765 - Sama_0590 0.66 -0.2 737,778 - Sama_0590 0.67 +0.3 737,872 + Sama_0590 0.71 -1.0 737,878 + Sama_0590 0.71 -0.2 737,878 + Sama_0590 0.71 -0.9 737,878 + Sama_0590 0.71 +0.1 737,879 - Sama_0590 0.71 -1.3 737,879 - Sama_0590 0.71 +2.4 737,879 - Sama_0590 0.71 -0.9 737,879 - Sama_0590 0.71 -0.5 737,879 - Sama_0590 0.71 -1.4 737,941 + Sama_0590 0.74 +0.2 737,942 - Sama_0590 0.74 -0.1 738,053 + Sama_0590 0.79 -0.3 738,053 + Sama_0590 0.79 -0.0 738,053 + Sama_0590 0.79 +1.6 738,054 - Sama_0590 0.79 +0.7 738,054 - Sama_0590 0.79 -1.3 738,066 + Sama_0590 0.80 -1.3 738,066 + Sama_0590 0.80 +0.9 738,067 - Sama_0590 0.80 +0.0 738,067 - Sama_0590 0.80 +0.4 738,106 + Sama_0590 0.81 -0.3 738,106 + Sama_0590 0.81 -0.2 738,106 + Sama_0590 0.81 -0.4 738,106 + Sama_0590 0.81 +0.1 738,107 - Sama_0590 0.81 +0.8 738,107 - Sama_0590 0.81 +0.2 738,107 - Sama_0590 0.81 -0.2 738,107 - Sama_0590 0.81 -0.3 738,107 - Sama_0590 0.81 +0.2 738,109 + Sama_0590 0.81 +0.2 738,109 + Sama_0590 0.81 +1.8 738,110 - Sama_0590 0.82 -1.0 738,110 - Sama_0590 0.82 -0.6 738,127 + Sama_0590 0.82 -0.4 738,127 + Sama_0590 0.82 +0.3 738,128 - Sama_0590 0.82 -0.2 738,128 - Sama_0590 0.82 -1.4 738,128 - Sama_0590 0.82 +1.4 738,128 - Sama_0590 0.82 -2.0 738,226 + Sama_0590 0.87 +0.1 738,226 + Sama_0590 0.87 -0.2 738,227 - Sama_0590 0.87 +0.4 738,245 + Sama_0590 0.88 +0.5 738,245 + Sama_0590 0.88 -0.1 738,246 - Sama_0590 0.88 +0.0 738,246 - Sama_0590 0.88 -0.5 738,246 - Sama_0590 0.88 +0.5 738,246 - Sama_0590 0.88 -1.3 738,247 + Sama_0590 0.88 -1.6 738,247 + Sama_0590 0.88 +1.0 738,247 + Sama_0590 0.88 +0.4 738,247 + Sama_0590 0.88 -0.9 738,247 + Sama_0590 0.88 -0.2 738,248 - Sama_0590 0.88 -1.6 738,248 - Sama_0590 0.88 -0.4 738,248 - Sama_0590 0.88 +0.9 738,248 - Sama_0590 0.88 +0.6 738,248 - Sama_0590 0.88 -0.6 738,248 - Sama_0590 0.88 -0.4 738,289 + Sama_0590 0.90 +0.7 738,289 + Sama_0590 0.90 +0.5 738,289 + Sama_0590 0.90 +0.4 738,290 - Sama_0590 0.90 -0.2 738,290 - Sama_0590 0.90 +0.5 738,290 - Sama_0590 0.90 +2.0 738,299 + +0.2 738,299 + +1.6 738,299 + +0.2 738,300 - -0.8 738,509 + -0.6 738,510 - -0.0 738,548 - -0.8 738,553 + -1.1 738,554 - -1.1 738,558 + -0.2 738,574 + +0.3 738,593 + +0.2 738,617 + -0.7 738,618 - +0.4 738,644 + -0.1 738,669 + +0.0 738,669 + -0.3 738,669 + -0.9 738,669 + +0.6 738,669 + +0.3 738,669 + +0.8 738,669 + -0.3 738,670 - +0.7 738,670 - -1.3 738,670 - -0.1 738,678 + +0.8 738,679 - -1.4 738,687 + +0.7 738,687 + +0.7 738,731 + +0.6 738,741 + -1.0 738,741 + +0.1 738,843 + Sama_0591 0.14 -0.4 738,843 + Sama_0591 0.14 -0.3 738,913 + Sama_0591 0.24 -2.6 738,913 + Sama_0591 0.24 -0.4 738,913 + Sama_0591 0.24 +0.3 738,913 + Sama_0591 0.24 +1.1 738,914 - Sama_0591 0.25 -2.1 738,914 - Sama_0591 0.25 +0.4 738,914 - Sama_0591 0.25 -0.0 738,957 - Sama_0591 0.31 -1.9 739,026 + Sama_0591 0.40 +0.5 739,033 + Sama_0591 0.41 -1.6 739,033 + Sama_0591 0.41 -1.3 739,034 - Sama_0591 0.42 -1.7 739,034 - Sama_0591 0.42 -1.0 739,034 - Sama_0591 0.42 -0.7 739,061 + Sama_0591 0.45 -0.5 739,061 + Sama_0591 0.45 +1.8 739,061 + Sama_0591 0.45 -0.9 739,062 - Sama_0591 0.46 -0.3 739,062 - Sama_0591 0.46 -1.1 739,062 - Sama_0591 0.46 -2.2 739,102 + Sama_0591 0.51 -0.9 739,102 + Sama_0591 0.51 -1.4 739,102 + Sama_0591 0.51 +0.8 739,102 + Sama_0591 0.51 -0.2 739,102 + Sama_0591 0.51 -1.4 739,102 + Sama_0591 0.51 -0.5 739,102 + Sama_0591 0.51 -0.4 739,102 + Sama_0591 0.51 -0.9 739,102 + Sama_0591 0.51 -1.0 739,102 + Sama_0591 0.51 -1.3 739,102 + Sama_0591 0.51 -0.9 739,102 + Sama_0591 0.51 -0.1 739,103 - Sama_0591 0.51 -0.2 739,103 - Sama_0591 0.51 -1.3 739,103 - Sama_0591 0.51 -0.4 739,103 - Sama_0591 0.51 -0.4 739,103 - Sama_0591 0.51 -2.0 739,103 - Sama_0591 0.51 -0.9 739,103 - Sama_0591 0.51 +0.1 739,103 - Sama_0591 0.51 -0.9 739,103 - Sama_0591 0.51 -0.4 739,103 - Sama_0591 0.51 -2.1 739,122 + Sama_0591 0.54 +0.5 739,123 - Sama_0591 0.54 +0.1 739,123 - Sama_0591 0.54 -2.4 739,123 - Sama_0591 0.54 +0.6 739,128 + Sama_0591 0.55 -0.2 739,129 - Sama_0591 0.55 +0.6 739,129 - Sama_0591 0.55 +0.4 739,129 - Sama_0591 0.55 -2.6 739,173 + Sama_0591 0.61 -0.5 739,198 + Sama_0591 0.65 -0.9 739,198 + Sama_0591 0.65 -0.0 739,199 - Sama_0591 0.65 +0.2 739,199 - Sama_0591 0.65 -0.5 739,206 + Sama_0591 0.66 +0.7 739,318 - Sama_0591 0.82 +0.7 739,329 + Sama_0591 0.84 +0.0 739,329 + Sama_0591 0.84 -0.5 739,329 + Sama_0591 0.84 -0.1 739,329 + Sama_0591 0.84 -1.0 739,330 - Sama_0591 0.84 -1.3 739,330 - Sama_0591 0.84 -0.9 739,330 - Sama_0591 0.84 -0.4 739,330 - Sama_0591 0.84 -1.3 739,330 - Sama_0591 0.84 +0.6 739,330 - Sama_0591 0.84 +0.7 739,351 - Sama_0591 0.87 +0.7 739,404 + +1.2 739,417 + -0.1 739,417 + -0.0 739,417 + -0.4 739,441 + -0.2 739,459 - +0.3 739,490 + -0.2 739,491 - -0.0 739,541 + -0.8 739,546 + -0.2 739,546 + -1.2 739,546 + +0.4 739,546 + -0.0 739,547 - -0.5 739,547 - -0.3 739,549 + +1.4 739,549 + -0.4 739,549 + +0.1 739,549 + -0.1 739,550 - +1.1 739,550 - -0.6 739,550 - -0.1 739,550 - -0.6 739,550 - -1.3 739,550 - -0.2 739,550 - +0.5 739,550 - +0.2 739,583 + +0.5 739,583 + +0.4 739,583 + +0.4 739,583 + -0.3 739,583 + -0.5 739,583 + -0.2 739,584 - -1.1 739,584 - +0.5 739,584 - +0.1 739,584 - -0.5 739,584 - -1.1 739,584 - -0.3 739,587 + -0.1 739,587 + +0.7 739,588 - +0.4 739,588 - -1.2 739,588 - +0.2 739,593 + -0.2 739,594 - -0.5 739,603 + -0.1 739,603 + -0.3 739,604 - +0.1 739,604 - -1.0 739,604 - +1.4 739,668 + -0.7 739,674 + +0.8 739,675 - +0.0 739,684 + -1.5 739,684 + -0.3 739,684 + -0.5 739,685 - -1.4 739,685 - -1.2 739,685 - -0.9 739,754 - -0.0 739,759 + -0.1 739,759 + +0.7 739,759 + -0.2 739,759 + +0.3 739,759 + -0.2 739,760 - -0.0 739,827 - +0.7 739,875 - +0.3 739,895 + -0.6 739,895 + +0.7 739,895 + -0.5 739,895 + +1.0 739,895 + +0.5 739,895 + +0.7 739,895 + +0.1 739,896 - -1.0 739,896 - +0.8 739,896 - +1.2 739,896 - -1.6 739,896 - -0.1 739,896 - -0.8 739,897 + -0.7 739,898 - +0.3 739,904 + -0.0 739,905 - -0.1 739,934 + +0.0 739,935 - -1.0 739,947 + -1.5 739,947 + +0.2 739,947 + -2.2 739,947 + -0.4 739,947 + +0.0 739,972 + +0.2 739,972 + -0.4 740,015 + -1.4 740,016 - +1.0 740,016 - +0.5 740,016 - +0.4 740,016 - -0.4 740,137 + Sama_0592 0.12 +0.7 740,138 - Sama_0592 0.12 -0.6 740,188 + Sama_0592 0.14 -0.7 740,188 + Sama_0592 0.14 -0.9 740,188 + Sama_0592 0.14 +0.4 740,189 - Sama_0592 0.14 -0.2 740,195 + Sama_0592 0.14 -0.3 740,195 + Sama_0592 0.14 +0.2 740,195 + Sama_0592 0.14 -1.0 740,195 + Sama_0592 0.14 +1.4 740,195 + Sama_0592 0.14 -0.0 740,195 + Sama_0592 0.14 +0.7 740,196 - Sama_0592 0.14 +0.4 740,196 - Sama_0592 0.14 +0.1 740,235 + Sama_0592 0.15 -1.0 740,235 + Sama_0592 0.15 -1.8 740,236 - Sama_0592 0.15 +0.0 740,334 + Sama_0592 0.18 +0.6 740,356 + Sama_0592 0.18 +0.4 740,398 + Sama_0592 0.20 +0.6 740,398 + Sama_0592 0.20 +0.3 740,398 + Sama_0592 0.20 +0.2 740,398 + Sama_0592 0.20 +0.9
Or see this region's nucleotide sequence