Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aceE and aceF are separated by 11 nucleotides aceF and Sama_0377 are separated by 170 nucleotides
Sama_0375: aceE - pyruvate dehydrogenase subunit E1 (RefSeq), at 453,823 to 456,519
aceE
Sama_0376: aceF - dihydrolipoamide acetyltransferase (RefSeq), at 456,531 to 458,459
aceF
Sama_0377: Sama_0377 - dihydrolipoamide dehydrogenase (RefSeq), at 458,630 to 460,060
_0377
Position (kb) 456
457
458
459 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 455.538 kb on + strand, within aceE at 455.538 kb on + strand, within aceE at 455.620 kb on + strand, within aceE at 455.620 kb on + strand, within aceE at 455.620 kb on + strand, within aceE at 455.621 kb on - strand, within aceE at 455.621 kb on - strand, within aceE at 455.626 kb on + strand, within aceE at 455.626 kb on + strand, within aceE at 455.626 kb on + strand, within aceE at 455.627 kb on - strand, within aceE at 455.627 kb on - strand, within aceE at 455.627 kb on - strand, within aceE at 455.629 kb on + strand, within aceE at 455.629 kb on + strand, within aceE at 455.629 kb on + strand, within aceE at 455.630 kb on - strand, within aceE at 455.725 kb on - strand, within aceE at 455.793 kb on + strand, within aceE at 455.794 kb on - strand, within aceE at 455.799 kb on + strand, within aceE at 455.800 kb on - strand, within aceE at 455.800 kb on - strand, within aceE at 455.825 kb on - strand, within aceE at 455.866 kb on + strand, within aceE at 455.866 kb on + strand, within aceE at 455.866 kb on + strand, within aceE at 455.866 kb on + strand, within aceE at 455.866 kb on + strand, within aceE at 455.866 kb on + strand, within aceE at 455.867 kb on - strand, within aceE at 455.867 kb on - strand, within aceE at 455.867 kb on - strand, within aceE at 455.890 kb on + strand, within aceE at 455.890 kb on + strand, within aceE at 455.891 kb on - strand, within aceE at 455.917 kb on + strand, within aceE at 455.971 kb on + strand, within aceE at 455.971 kb on + strand, within aceE at 455.989 kb on - strand, within aceE at 456.010 kb on + strand, within aceE at 456.010 kb on + strand, within aceE at 456.010 kb on + strand, within aceE at 456.071 kb on + strand, within aceE at 456.071 kb on + strand, within aceE at 456.072 kb on - strand, within aceE at 456.072 kb on - strand, within aceE at 456.111 kb on - strand, within aceE at 456.111 kb on - strand, within aceE at 456.183 kb on + strand, within aceE at 456.183 kb on + strand, within aceE at 456.183 kb on + strand, within aceE at 456.184 kb on - strand, within aceE at 456.217 kb on + strand, within aceE at 456.217 kb on + strand, within aceE at 456.217 kb on + strand, within aceE at 456.217 kb on + strand, within aceE at 456.217 kb on + strand, within aceE at 456.217 kb on + strand, within aceE at 456.218 kb on - strand, within aceE at 456.218 kb on - strand, within aceE at 456.218 kb on - strand, within aceE at 456.265 kb on + strand at 456.278 kb on - strand at 456.316 kb on + strand at 456.316 kb on + strand at 456.697 kb on - strand at 456.854 kb on - strand, within aceF at 456.854 kb on - strand, within aceF at 456.949 kb on + strand, within aceF at 456.949 kb on + strand, within aceF at 456.968 kb on + strand, within aceF at 457.029 kb on - strand, within aceF at 457.286 kb on + strand, within aceF at 457.287 kb on - strand, within aceF at 457.294 kb on + strand, within aceF at 457.320 kb on - strand, within aceF at 457.384 kb on + strand, within aceF at 457.496 kb on + strand, within aceF at 457.496 kb on + strand, within aceF at 457.599 kb on - strand, within aceF at 457.622 kb on - strand, within aceF at 457.641 kb on + strand, within aceF at 457.642 kb on - strand, within aceF at 457.820 kb on + strand, within aceF at 458.043 kb on + strand, within aceF at 458.044 kb on - strand, within aceF at 458.044 kb on - strand, within aceF at 458.124 kb on + strand, within aceF at 458.124 kb on + strand, within aceF at 458.124 kb on + strand, within aceF at 458.180 kb on + strand, within aceF at 458.210 kb on + strand, within aceF at 458.211 kb on - strand, within aceF at 458.415 kb on + strand at 458.416 kb on - strand at 458.416 kb on - strand at 458.429 kb on - strand at 458.440 kb on + strand at 458.440 kb on + strand at 458.464 kb on - strand at 458.479 kb on + strand at 458.479 kb on + strand at 458.480 kb on - strand at 458.493 kb on + strand at 458.493 kb on + strand at 458.494 kb on - strand at 458.519 kb on + strand at 458.535 kb on + strand at 458.535 kb on + strand at 458.535 kb on + strand at 458.535 kb on + strand at 458.535 kb on + strand at 458.549 kb on + strand at 458.549 kb on + strand at 458.558 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 455,538 + aceE Sama_0375 0.64 +1.3 455,538 + aceE Sama_0375 0.64 -0.8 455,620 + aceE Sama_0375 0.67 -0.4 455,620 + aceE Sama_0375 0.67 -0.2 455,620 + aceE Sama_0375 0.67 +0.2 455,621 - aceE Sama_0375 0.67 -1.0 455,621 - aceE Sama_0375 0.67 -1.0 455,626 + aceE Sama_0375 0.67 +0.6 455,626 + aceE Sama_0375 0.67 -0.4 455,626 + aceE Sama_0375 0.67 +0.1 455,627 - aceE Sama_0375 0.67 -0.2 455,627 - aceE Sama_0375 0.67 -1.4 455,627 - aceE Sama_0375 0.67 +0.6 455,629 + aceE Sama_0375 0.67 +1.6 455,629 + aceE Sama_0375 0.67 +0.9 455,629 + aceE Sama_0375 0.67 +1.6 455,630 - aceE Sama_0375 0.67 +0.8 455,725 - aceE Sama_0375 0.71 +1.0 455,793 + aceE Sama_0375 0.73 -0.5 455,794 - aceE Sama_0375 0.73 +1.0 455,799 + aceE Sama_0375 0.73 -0.6 455,800 - aceE Sama_0375 0.73 +0.9 455,800 - aceE Sama_0375 0.73 +0.6 455,825 - aceE Sama_0375 0.74 -1.2 455,866 + aceE Sama_0375 0.76 +1.9 455,866 + aceE Sama_0375 0.76 +2.6 455,866 + aceE Sama_0375 0.76 -0.4 455,866 + aceE Sama_0375 0.76 +0.6 455,866 + aceE Sama_0375 0.76 +0.3 455,866 + aceE Sama_0375 0.76 +1.1 455,867 - aceE Sama_0375 0.76 +0.2 455,867 - aceE Sama_0375 0.76 -1.0 455,867 - aceE Sama_0375 0.76 -0.2 455,890 + aceE Sama_0375 0.77 -0.9 455,890 + aceE Sama_0375 0.77 -0.1 455,891 - aceE Sama_0375 0.77 +1.0 455,917 + aceE Sama_0375 0.78 -0.0 455,971 + aceE Sama_0375 0.80 +1.3 455,971 + aceE Sama_0375 0.80 -0.4 455,989 - aceE Sama_0375 0.80 +0.2 456,010 + aceE Sama_0375 0.81 -0.8 456,010 + aceE Sama_0375 0.81 +1.1 456,010 + aceE Sama_0375 0.81 -1.0 456,071 + aceE Sama_0375 0.83 -0.2 456,071 + aceE Sama_0375 0.83 +1.8 456,072 - aceE Sama_0375 0.83 +0.6 456,072 - aceE Sama_0375 0.83 +0.3 456,111 - aceE Sama_0375 0.85 -0.2 456,111 - aceE Sama_0375 0.85 -1.2 456,183 + aceE Sama_0375 0.88 -0.3 456,183 + aceE Sama_0375 0.88 +0.5 456,183 + aceE Sama_0375 0.88 +0.2 456,184 - aceE Sama_0375 0.88 -1.0 456,217 + aceE Sama_0375 0.89 +0.2 456,217 + aceE Sama_0375 0.89 +1.6 456,217 + aceE Sama_0375 0.89 +1.2 456,217 + aceE Sama_0375 0.89 -1.5 456,217 + aceE Sama_0375 0.89 +1.6 456,217 + aceE Sama_0375 0.89 -0.4 456,218 - aceE Sama_0375 0.89 -0.4 456,218 - aceE Sama_0375 0.89 +0.8 456,218 - aceE Sama_0375 0.89 +1.6 456,265 + +1.0 456,278 - -0.4 456,316 + -0.8 456,316 + +0.6 456,697 - -0.8 456,854 - aceF Sama_0376 0.17 -0.2 456,854 - aceF Sama_0376 0.17 +1.0 456,949 + aceF Sama_0376 0.22 -1.0 456,949 + aceF Sama_0376 0.22 -1.6 456,968 + aceF Sama_0376 0.23 -1.4 457,029 - aceF Sama_0376 0.26 -1.2 457,286 + aceF Sama_0376 0.39 -0.0 457,287 - aceF Sama_0376 0.39 -1.2 457,294 + aceF Sama_0376 0.40 -1.2 457,320 - aceF Sama_0376 0.41 +1.0 457,384 + aceF Sama_0376 0.44 +0.1 457,496 + aceF Sama_0376 0.50 +0.2 457,496 + aceF Sama_0376 0.50 -0.0 457,599 - aceF Sama_0376 0.55 -1.2 457,622 - aceF Sama_0376 0.57 -0.4 457,641 + aceF Sama_0376 0.58 +1.0 457,642 - aceF Sama_0376 0.58 +0.8 457,820 + aceF Sama_0376 0.67 -1.6 458,043 + aceF Sama_0376 0.78 +0.9 458,044 - aceF Sama_0376 0.78 -0.8 458,044 - aceF Sama_0376 0.78 +0.6 458,124 + aceF Sama_0376 0.83 +1.0 458,124 + aceF Sama_0376 0.83 -0.0 458,124 + aceF Sama_0376 0.83 -1.0 458,180 + aceF Sama_0376 0.85 -1.8 458,210 + aceF Sama_0376 0.87 -0.5 458,211 - aceF Sama_0376 0.87 +0.3 458,415 + -1.6 458,416 - +1.6 458,416 - +0.8 458,429 - +0.6 458,440 + +0.1 458,440 + -0.6 458,464 - +0.3 458,479 + +3.1 458,479 + -0.3 458,480 - +0.2 458,493 + -0.3 458,493 + -1.4 458,494 - +0.0 458,519 + -0.9 458,535 + +0.6 458,535 + +0.1 458,535 + -0.9 458,535 + +0.4 458,535 + -0.3 458,549 + +0.3 458,549 + -0.3 458,558 + +0.2
Or see this region's nucleotide sequence