Experiment: Cytidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliS and SO3234 are separated by 6 nucleotides SO3234 and fliD are separated by 28 nucleotides fliD and flaG are separated by 42 nucleotides flaG and SO3237 are separated by 156 nucleotides
SO3233: fliS - flagellar protein FliS (NCBI ptt file), at 3,373,086 to 3,373,496
fliS
SO3234: SO3234 - hypothetical protein (NCBI ptt file), at 3,373,503 to 3,373,823
SO3234
SO3235: fliD - flagellar hook-associated protein FliD (NCBI ptt file), at 3,373,852 to 3,375,222
fliD
SO3236: flaG - flagellin FlaG (NCBI ptt file), at 3,375,265 to 3,375,624
flaG
SO3237: SO3237 - flagellin (NCBI ptt file), at 3,375,781 to 3,376,599
SO3237
Position (kb)
3373
3374
3375
3376 Strain fitness (log2 ratio)
-1
0
1
2 at 3372.884 kb on - strand at 3372.901 kb on - strand at 3372.917 kb on + strand at 3373.116 kb on + strand at 3373.116 kb on + strand at 3373.116 kb on + strand at 3373.136 kb on + strand, within fliS at 3373.145 kb on - strand, within fliS at 3373.156 kb on - strand, within fliS at 3373.361 kb on + strand, within fliS at 3373.370 kb on + strand, within fliS at 3373.377 kb on + strand, within fliS at 3373.377 kb on + strand, within fliS at 3373.385 kb on - strand, within fliS at 3373.385 kb on - strand, within fliS at 3373.385 kb on - strand, within fliS at 3373.389 kb on + strand, within fliS at 3373.389 kb on + strand, within fliS at 3373.397 kb on - strand, within fliS at 3373.400 kb on - strand, within fliS at 3373.426 kb on - strand, within fliS at 3373.500 kb on + strand at 3373.500 kb on - strand at 3373.539 kb on - strand, within SO3234 at 3373.624 kb on + strand, within SO3234 at 3373.684 kb on - strand, within SO3234 at 3373.711 kb on - strand, within SO3234 at 3373.711 kb on - strand, within SO3234 at 3373.733 kb on + strand, within SO3234 at 3373.749 kb on + strand, within SO3234 at 3373.775 kb on + strand, within SO3234 at 3373.775 kb on + strand, within SO3234 at 3373.783 kb on - strand, within SO3234 at 3373.801 kb on - strand at 3374.020 kb on + strand, within fliD at 3374.028 kb on - strand, within fliD at 3374.036 kb on - strand, within fliD at 3374.178 kb on + strand, within fliD at 3374.323 kb on + strand, within fliD at 3374.331 kb on - strand, within fliD at 3374.371 kb on - strand, within fliD at 3374.468 kb on + strand, within fliD at 3374.642 kb on - strand, within fliD at 3374.642 kb on - strand, within fliD at 3374.698 kb on + strand, within fliD at 3374.740 kb on + strand, within fliD at 3374.977 kb on + strand, within fliD at 3374.982 kb on + strand, within fliD at 3375.121 kb on + strand at 3375.184 kb on + strand at 3375.196 kb on + strand at 3375.262 kb on + strand at 3375.629 kb on - strand at 3375.715 kb on + strand at 3375.966 kb on - strand, within SO3237 at 3375.981 kb on - strand, within SO3237 at 3375.983 kb on + strand, within SO3237 at 3376.122 kb on + strand, within SO3237 at 3376.196 kb on + strand, within SO3237
Per-strain Table
Position Strand Gene LocusTag Fraction Cytidine (C) remove 3,372,884 - +2.6 3,372,901 - -0.5 3,372,917 + +2.0 3,373,116 + +0.2 3,373,116 + +1.6 3,373,116 + +0.2 3,373,136 + fliS SO3233 0.12 +1.2 3,373,145 - fliS SO3233 0.14 +0.3 3,373,156 - fliS SO3233 0.17 +1.0 3,373,361 + fliS SO3233 0.67 +2.6 3,373,370 + fliS SO3233 0.69 +2.7 3,373,377 + fliS SO3233 0.71 +1.9 3,373,377 + fliS SO3233 0.71 +2.4 3,373,385 - fliS SO3233 0.73 +1.7 3,373,385 - fliS SO3233 0.73 +1.7 3,373,385 - fliS SO3233 0.73 +1.6 3,373,389 + fliS SO3233 0.74 +2.4 3,373,389 + fliS SO3233 0.74 +2.1 3,373,397 - fliS SO3233 0.76 +1.0 3,373,400 - fliS SO3233 0.76 +1.8 3,373,426 - fliS SO3233 0.83 +1.8 3,373,500 + +2.0 3,373,500 - -1.2 3,373,539 - SO3234 0.11 +0.4 3,373,624 + SO3234 0.38 +1.8 3,373,684 - SO3234 0.56 +0.9 3,373,711 - SO3234 0.65 -0.6 3,373,711 - SO3234 0.65 +0.7 3,373,733 + SO3234 0.72 +0.4 3,373,749 + SO3234 0.77 +1.0 3,373,775 + SO3234 0.85 +1.8 3,373,775 + SO3234 0.85 +0.8 3,373,783 - SO3234 0.87 +0.8 3,373,801 - +0.8 3,374,020 + fliD SO3235 0.12 +1.2 3,374,028 - fliD SO3235 0.13 -0.3 3,374,036 - fliD SO3235 0.13 +0.7 3,374,178 + fliD SO3235 0.24 +1.9 3,374,323 + fliD SO3235 0.34 +1.4 3,374,331 - fliD SO3235 0.35 +0.5 3,374,371 - fliD SO3235 0.38 +0.9 3,374,468 + fliD SO3235 0.45 +1.3 3,374,642 - fliD SO3235 0.58 +0.6 3,374,642 - fliD SO3235 0.58 +0.9 3,374,698 + fliD SO3235 0.62 -0.0 3,374,740 + fliD SO3235 0.65 +1.0 3,374,977 + fliD SO3235 0.82 +1.3 3,374,982 + fliD SO3235 0.82 +1.1 3,375,121 + +1.3 3,375,184 + +1.0 3,375,196 + +1.4 3,375,262 + +1.2 3,375,629 - +0.2 3,375,715 + +0.6 3,375,966 - SO3237 0.23 -0.2 3,375,981 - SO3237 0.24 +1.7 3,375,983 + SO3237 0.25 +1.9 3,376,122 + SO3237 0.42 +2.5 3,376,196 + SO3237 0.51 +2.6
Or see this region's nucleotide sequence