Strain Fitness in Shewanella oneidensis MR-1 around SO3218

Experiment: Cytidine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliR and fliQ are separated by 4 nucleotidesfliQ and fliP are separated by 17 nucleotidesfliP and fliO overlap by 1 nucleotidesfliO and fliN are separated by 34 nucleotidesfliN and fliM are separated by 12 nucleotides SO3216: fliR - flagellar biosynthetic protein FliR (NCBI ptt file), at 3,356,767 to 3,357,564 fliR SO3217: fliQ - flagellar biosynthetic protein FliQ (NCBI ptt file), at 3,357,569 to 3,357,838 fliQ SO3218: fliP - flagellar biosynthetic protein FliP (NCBI ptt file), at 3,357,856 to 3,358,599 fliP SO3219: fliO - flagellar protein FliO (NCBI ptt file), at 3,358,599 to 3,359,006 fliO SO3220: fliN - flagellar motor switch protein FliN (NCBI ptt file), at 3,359,041 to 3,359,415 fliN SO3221: fliM - flagellar motor switch protein FliM (NCBI ptt file), at 3,359,428 to 3,360,459 fliM Position (kb) 3357 3358 3359Strain fitness (log2 ratio) -1 0 1 2 3at 3356.870 kb on - strand, within fliRat 3357.018 kb on - strand, within fliRat 3357.069 kb on + strand, within fliRat 3357.072 kb on - strand, within fliRat 3357.310 kb on + strand, within fliRat 3357.405 kb on - strand, within fliRat 3357.433 kb on + strand, within fliRat 3357.517 kb on + strandat 3357.524 kb on - strandat 3357.623 kb on + strand, within fliQat 3357.623 kb on + strand, within fliQat 3357.670 kb on + strand, within fliQat 3357.697 kb on - strand, within fliQat 3357.697 kb on - strand, within fliQat 3357.744 kb on + strand, within fliQat 3357.758 kb on + strand, within fliQat 3357.826 kb on + strandat 3357.858 kb on + strandat 3357.861 kb on - strandat 3357.953 kb on - strand, within fliPat 3357.960 kb on + strand, within fliPat 3357.970 kb on - strand, within fliPat 3358.088 kb on - strand, within fliPat 3358.152 kb on + strand, within fliPat 3358.196 kb on + strand, within fliPat 3358.204 kb on - strand, within fliPat 3358.249 kb on - strand, within fliPat 3358.319 kb on - strand, within fliPat 3358.321 kb on + strand, within fliPat 3358.436 kb on - strand, within fliPat 3358.485 kb on - strand, within fliPat 3358.565 kb on + strandat 3358.594 kb on - strandat 3358.619 kb on + strandat 3358.757 kb on + strand, within fliOat 3358.757 kb on + strand, within fliOat 3358.861 kb on + strand, within fliOat 3358.962 kb on + strand, within fliOat 3359.028 kb on - strandat 3359.074 kb on + strandat 3359.082 kb on - strand, within fliNat 3359.157 kb on + strand, within fliNat 3359.178 kb on + strand, within fliNat 3359.180 kb on + strand, within fliNat 3359.299 kb on - strand, within fliNat 3359.300 kb on + strand, within fliNat 3359.327 kb on + strand, within fliNat 3359.336 kb on + strand, within fliNat 3359.401 kb on - strandat 3359.408 kb on + strandat 3359.518 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cytidine (C)
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3,356,870 - fliR SO3216 0.13 +2.0
3,357,018 - fliR SO3216 0.31 +1.7
3,357,069 + fliR SO3216 0.38 +2.3
3,357,072 - fliR SO3216 0.38 +2.2
3,357,310 + fliR SO3216 0.68 +1.8
3,357,405 - fliR SO3216 0.80 +1.9
3,357,433 + fliR SO3216 0.83 +2.5
3,357,517 + +2.3
3,357,524 - +1.1
3,357,623 + fliQ SO3217 0.20 +2.2
3,357,623 + fliQ SO3217 0.20 +2.7
3,357,670 + fliQ SO3217 0.37 +1.9
3,357,697 - fliQ SO3217 0.47 +1.6
3,357,697 - fliQ SO3217 0.47 +1.5
3,357,744 + fliQ SO3217 0.65 +3.1
3,357,758 + fliQ SO3217 0.70 +1.3
3,357,826 + +2.0
3,357,858 + +1.4
3,357,861 - +0.0
3,357,953 - fliP SO3218 0.13 +1.7
3,357,960 + fliP SO3218 0.14 +2.0
3,357,970 - fliP SO3218 0.15 +1.5
3,358,088 - fliP SO3218 0.31 +1.9
3,358,152 + fliP SO3218 0.40 +2.8
3,358,196 + fliP SO3218 0.46 +1.7
3,358,204 - fliP SO3218 0.47 +2.6
3,358,249 - fliP SO3218 0.53 +1.9
3,358,319 - fliP SO3218 0.62 +3.1
3,358,321 + fliP SO3218 0.62 +0.9
3,358,436 - fliP SO3218 0.78 +2.3
3,358,485 - fliP SO3218 0.85 +1.0
3,358,565 + +2.0
3,358,594 - +0.8
3,358,619 + +2.2
3,358,757 + fliO SO3219 0.39 +1.6
3,358,757 + fliO SO3219 0.39 +1.1
3,358,861 + fliO SO3219 0.64 +2.0
3,358,962 + fliO SO3219 0.89 +2.4
3,359,028 - +1.8
3,359,074 + +2.1
3,359,082 - fliN SO3220 0.11 +1.7
3,359,157 + fliN SO3220 0.31 +3.7
3,359,178 + fliN SO3220 0.37 +2.6
3,359,180 + fliN SO3220 0.37 +1.7
3,359,299 - fliN SO3220 0.69 +1.2
3,359,300 + fliN SO3220 0.69 +1.8
3,359,327 + fliN SO3220 0.76 +2.7
3,359,336 + fliN SO3220 0.79 +1.0
3,359,401 - +2.1
3,359,408 + +2.5
3,359,518 + +3.1

Or see this region's nucleotide sequence