Experiment: Cytidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliR and fliQ are separated by 4 nucleotides fliQ and fliP are separated by 17 nucleotides fliP and fliO overlap by 1 nucleotides fliO and fliN are separated by 34 nucleotides fliN and fliM are separated by 12 nucleotides
SO3216: fliR - flagellar biosynthetic protein FliR (NCBI ptt file), at 3,356,767 to 3,357,564
fliR
SO3217: fliQ - flagellar biosynthetic protein FliQ (NCBI ptt file), at 3,357,569 to 3,357,838
fliQ
SO3218: fliP - flagellar biosynthetic protein FliP (NCBI ptt file), at 3,357,856 to 3,358,599
fliP
SO3219: fliO - flagellar protein FliO (NCBI ptt file), at 3,358,599 to 3,359,006
fliO
SO3220: fliN - flagellar motor switch protein FliN (NCBI ptt file), at 3,359,041 to 3,359,415
fliN
SO3221: fliM - flagellar motor switch protein FliM (NCBI ptt file), at 3,359,428 to 3,360,459
fliM
Position (kb)
3357
3358
3359 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3356.870 kb on - strand, within fliR at 3357.018 kb on - strand, within fliR at 3357.069 kb on + strand, within fliR at 3357.072 kb on - strand, within fliR at 3357.310 kb on + strand, within fliR at 3357.405 kb on - strand, within fliR at 3357.433 kb on + strand, within fliR at 3357.517 kb on + strand at 3357.524 kb on - strand at 3357.623 kb on + strand, within fliQ at 3357.623 kb on + strand, within fliQ at 3357.670 kb on + strand, within fliQ at 3357.697 kb on - strand, within fliQ at 3357.697 kb on - strand, within fliQ at 3357.744 kb on + strand, within fliQ at 3357.758 kb on + strand, within fliQ at 3357.826 kb on + strand at 3357.858 kb on + strand at 3357.861 kb on - strand at 3357.953 kb on - strand, within fliP at 3357.960 kb on + strand, within fliP at 3357.970 kb on - strand, within fliP at 3358.088 kb on - strand, within fliP at 3358.152 kb on + strand, within fliP at 3358.196 kb on + strand, within fliP at 3358.204 kb on - strand, within fliP at 3358.249 kb on - strand, within fliP at 3358.319 kb on - strand, within fliP at 3358.321 kb on + strand, within fliP at 3358.436 kb on - strand, within fliP at 3358.485 kb on - strand, within fliP at 3358.565 kb on + strand at 3358.594 kb on - strand at 3358.619 kb on + strand at 3358.757 kb on + strand, within fliO at 3358.757 kb on + strand, within fliO at 3358.861 kb on + strand, within fliO at 3358.962 kb on + strand, within fliO at 3359.028 kb on - strand at 3359.074 kb on + strand at 3359.082 kb on - strand, within fliN at 3359.157 kb on + strand, within fliN at 3359.178 kb on + strand, within fliN at 3359.180 kb on + strand, within fliN at 3359.299 kb on - strand, within fliN at 3359.300 kb on + strand, within fliN at 3359.327 kb on + strand, within fliN at 3359.336 kb on + strand, within fliN at 3359.401 kb on - strand at 3359.408 kb on + strand at 3359.518 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cytidine (C) remove 3,356,870 - fliR SO3216 0.13 +2.0 3,357,018 - fliR SO3216 0.31 +1.7 3,357,069 + fliR SO3216 0.38 +2.3 3,357,072 - fliR SO3216 0.38 +2.2 3,357,310 + fliR SO3216 0.68 +1.8 3,357,405 - fliR SO3216 0.80 +1.9 3,357,433 + fliR SO3216 0.83 +2.5 3,357,517 + +2.3 3,357,524 - +1.1 3,357,623 + fliQ SO3217 0.20 +2.2 3,357,623 + fliQ SO3217 0.20 +2.7 3,357,670 + fliQ SO3217 0.37 +1.9 3,357,697 - fliQ SO3217 0.47 +1.6 3,357,697 - fliQ SO3217 0.47 +1.5 3,357,744 + fliQ SO3217 0.65 +3.1 3,357,758 + fliQ SO3217 0.70 +1.3 3,357,826 + +2.0 3,357,858 + +1.4 3,357,861 - +0.0 3,357,953 - fliP SO3218 0.13 +1.7 3,357,960 + fliP SO3218 0.14 +2.0 3,357,970 - fliP SO3218 0.15 +1.5 3,358,088 - fliP SO3218 0.31 +1.9 3,358,152 + fliP SO3218 0.40 +2.8 3,358,196 + fliP SO3218 0.46 +1.7 3,358,204 - fliP SO3218 0.47 +2.6 3,358,249 - fliP SO3218 0.53 +1.9 3,358,319 - fliP SO3218 0.62 +3.1 3,358,321 + fliP SO3218 0.62 +0.9 3,358,436 - fliP SO3218 0.78 +2.3 3,358,485 - fliP SO3218 0.85 +1.0 3,358,565 + +2.0 3,358,594 - +0.8 3,358,619 + +2.2 3,358,757 + fliO SO3219 0.39 +1.6 3,358,757 + fliO SO3219 0.39 +1.1 3,358,861 + fliO SO3219 0.64 +2.0 3,358,962 + fliO SO3219 0.89 +2.4 3,359,028 - +1.8 3,359,074 + +2.1 3,359,082 - fliN SO3220 0.11 +1.7 3,359,157 + fliN SO3220 0.31 +3.7 3,359,178 + fliN SO3220 0.37 +2.6 3,359,180 + fliN SO3220 0.37 +1.7 3,359,299 - fliN SO3220 0.69 +1.2 3,359,300 + fliN SO3220 0.69 +1.8 3,359,327 + fliN SO3220 0.76 +2.7 3,359,336 + fliN SO3220 0.79 +1.0 3,359,401 - +2.1 3,359,408 + +2.5 3,359,518 + +3.1
Or see this region's nucleotide sequence