Experiment: Cytidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpE and trpG are separated by 10 nucleotides trpG and trpD overlap by 1 nucleotides trpD and trpC/F are separated by 92 nucleotides
SO3019: trpE - anthranilate synthase component I (NCBI ptt file), at 3,129,858 to 3,131,582
trpE
SO3020: trpG - glutamine amido-transferase (NCBI ptt file), at 3,131,593 to 3,132,201
trpG
SO3021: trpD - anthranilate phosphoribosyltransferase (NCBI ptt file), at 3,132,201 to 3,133,244
trpD
SO3022: trpC/F - indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase (NCBI ptt file), at 3,133,337 to 3,134,746
trpC/F
Position (kb)
3132
3133
3134 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 3131.217 kb on + strand, within trpE at 3131.225 kb on - strand, within trpE at 3131.225 kb on - strand, within trpE at 3131.225 kb on - strand, within trpE at 3131.343 kb on - strand, within trpE at 3131.403 kb on - strand, within trpE at 3131.403 kb on - strand, within trpE at 3131.408 kb on - strand, within trpE at 3131.408 kb on - strand, within trpE at 3131.414 kb on - strand at 3131.416 kb on + strand at 3131.435 kb on - strand at 3131.511 kb on + strand at 3131.519 kb on - strand at 3131.555 kb on + strand at 3131.576 kb on + strand at 3131.584 kb on - strand at 3131.743 kb on + strand, within trpG at 3131.743 kb on + strand, within trpG at 3131.751 kb on - strand, within trpG at 3131.770 kb on - strand, within trpG at 3132.075 kb on + strand, within trpG at 3132.120 kb on - strand, within trpG at 3132.121 kb on + strand, within trpG at 3132.121 kb on + strand, within trpG at 3132.129 kb on - strand, within trpG at 3132.129 kb on - strand, within trpG at 3132.129 kb on - strand, within trpG at 3132.260 kb on - strand at 3132.281 kb on + strand at 3132.288 kb on + strand at 3132.331 kb on + strand, within trpD at 3132.331 kb on + strand, within trpD at 3132.412 kb on - strand, within trpD at 3132.418 kb on - strand, within trpD at 3132.419 kb on - strand, within trpD at 3132.504 kb on + strand, within trpD at 3132.596 kb on - strand, within trpD at 3132.625 kb on + strand, within trpD at 3132.625 kb on + strand, within trpD at 3132.646 kb on + strand, within trpD at 3132.646 kb on + strand, within trpD at 3132.646 kb on + strand, within trpD at 3132.654 kb on - strand, within trpD at 3132.654 kb on - strand, within trpD at 3132.681 kb on + strand, within trpD at 3132.711 kb on + strand, within trpD at 3132.775 kb on - strand, within trpD at 3132.801 kb on + strand, within trpD at 3132.809 kb on - strand, within trpD at 3132.811 kb on + strand, within trpD at 3132.843 kb on + strand, within trpD at 3132.873 kb on + strand, within trpD at 3132.932 kb on + strand, within trpD at 3133.085 kb on - strand, within trpD at 3133.174 kb on - strand at 3133.229 kb on - strand at 3133.230 kb on - strand at 3133.268 kb on + strand at 3133.337 kb on + strand at 3133.337 kb on + strand at 3133.345 kb on - strand at 3133.406 kb on + strand at 3133.407 kb on + strand at 3133.415 kb on - strand at 3133.540 kb on - strand, within trpC/F at 3133.540 kb on - strand, within trpC/F at 3133.659 kb on + strand, within trpC/F at 3133.766 kb on + strand, within trpC/F at 3133.945 kb on + strand, within trpC/F at 3134.058 kb on + strand, within trpC/F at 3134.139 kb on + strand, within trpC/F
Per-strain Table
Position Strand Gene LocusTag Fraction Cytidine (C) remove 3,131,217 + trpE SO3019 0.79 -3.9 3,131,225 - trpE SO3019 0.79 -2.6 3,131,225 - trpE SO3019 0.79 -2.9 3,131,225 - trpE SO3019 0.79 -2.9 3,131,343 - trpE SO3019 0.86 -5.1 3,131,403 - trpE SO3019 0.90 -2.3 3,131,403 - trpE SO3019 0.90 -5.2 3,131,408 - trpE SO3019 0.90 -4.4 3,131,408 - trpE SO3019 0.90 -3.6 3,131,414 - -6.3 3,131,416 + -5.4 3,131,435 - -4.3 3,131,511 + -0.5 3,131,519 - -5.3 3,131,555 + +0.6 3,131,576 + -1.1 3,131,584 - -4.3 3,131,743 + trpG SO3020 0.25 -2.3 3,131,743 + trpG SO3020 0.25 -2.6 3,131,751 - trpG SO3020 0.26 -2.8 3,131,770 - trpG SO3020 0.29 -6.3 3,132,075 + trpG SO3020 0.79 -1.5 3,132,120 - trpG SO3020 0.87 -3.6 3,132,121 + trpG SO3020 0.87 -1.3 3,132,121 + trpG SO3020 0.87 -0.8 3,132,129 - trpG SO3020 0.88 -3.7 3,132,129 - trpG SO3020 0.88 -4.6 3,132,129 - trpG SO3020 0.88 -5.9 3,132,260 - -5.7 3,132,281 + -3.2 3,132,288 + -6.6 3,132,331 + trpD SO3021 0.12 -4.2 3,132,331 + trpD SO3021 0.12 -3.8 3,132,412 - trpD SO3021 0.20 -3.3 3,132,418 - trpD SO3021 0.21 -3.8 3,132,419 - trpD SO3021 0.21 -5.1 3,132,504 + trpD SO3021 0.29 -4.9 3,132,596 - trpD SO3021 0.38 -2.9 3,132,625 + trpD SO3021 0.41 -4.9 3,132,625 + trpD SO3021 0.41 -4.6 3,132,646 + trpD SO3021 0.43 -2.6 3,132,646 + trpD SO3021 0.43 -4.5 3,132,646 + trpD SO3021 0.43 -3.2 3,132,654 - trpD SO3021 0.43 -1.3 3,132,654 - trpD SO3021 0.43 -6.9 3,132,681 + trpD SO3021 0.46 -3.7 3,132,711 + trpD SO3021 0.49 -3.9 3,132,775 - trpD SO3021 0.55 -0.8 3,132,801 + trpD SO3021 0.57 -3.8 3,132,809 - trpD SO3021 0.58 -4.0 3,132,811 + trpD SO3021 0.58 -5.7 3,132,843 + trpD SO3021 0.61 -5.1 3,132,873 + trpD SO3021 0.64 -3.1 3,132,932 + trpD SO3021 0.70 -4.6 3,133,085 - trpD SO3021 0.85 -4.5 3,133,174 - -5.3 3,133,229 - -4.9 3,133,230 - -3.8 3,133,268 + -2.0 3,133,337 + -6.8 3,133,337 + -5.5 3,133,345 - -1.3 3,133,406 + -4.6 3,133,407 + -3.8 3,133,415 - -3.7 3,133,540 - trpC/F SO3022 0.14 -6.5 3,133,540 - trpC/F SO3022 0.14 -1.8 3,133,659 + trpC/F SO3022 0.23 -4.4 3,133,766 + trpC/F SO3022 0.30 -3.5 3,133,945 + trpC/F SO3022 0.43 -3.8 3,134,058 + trpC/F SO3022 0.51 -3.6 3,134,139 + trpC/F SO3022 0.57 -3.1
Or see this region's nucleotide sequence