Experiment: Nalidixic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2826 and Psest_2827 are separated by 184 nucleotides Psest_2827 and Psest_2828 are separated by 165 nucleotides Psest_2828 and Psest_2829 overlap by 1 nucleotides
Psest_2826: Psest_2826 - acetyl-CoA carboxylase, carboxyl transferase, alpha subunit, at 3,014,471 to 3,015,421
_2826
Psest_2827: Psest_2827 - DNA-directed DNA polymerase III (polc), at 3,015,606 to 3,019,127
_2827
Psest_2828: Psest_2828 - Ribonuclease HII, at 3,019,293 to 3,019,931
_2828
Psest_2829: Psest_2829 - lipid-A-disaccharide synthase, at 3,019,931 to 3,021,064
_2829
Position (kb)
3015
3016
3017
3018
3019
3020 Strain fitness (log2 ratio)
-1
0
1 at 3019.168 kb on + strand at 3019.169 kb on - strand at 3019.217 kb on + strand at 3019.218 kb on - strand at 3019.250 kb on + strand at 3019.250 kb on + strand at 3019.250 kb on + strand at 3019.251 kb on - strand at 3019.251 kb on - strand at 3019.251 kb on - strand at 3019.295 kb on - strand at 3019.625 kb on + strand, within Psest_2828
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.001 mg/ml remove 3,019,168 + -0.6 3,019,169 - -0.6 3,019,217 + -0.4 3,019,218 - -0.6 3,019,250 + -0.9 3,019,250 + -1.7 3,019,250 + +0.2 3,019,251 - +1.3 3,019,251 - -0.3 3,019,251 - +0.7 3,019,295 - +0.3 3,019,625 + Psest_2828 0.52 +0.6
Or see this region's nucleotide sequence