Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt miaA and mutL are separated by 1 nucleotides mutL and Sama_3028 are separated by 14 nucleotides
Sama_3026: miaA - tRNA delta(2)-isopentenylpyrophosphate transferase (RefSeq), at 3,600,050 to 3,600,970
miaA
Sama_3027: mutL - DNA mismatch repair protein (RefSeq), at 3,600,972 to 3,602,924
mutL
Sama_3028: Sama_3028 - N-acetylmuramoyl-L-alanine amidase (RefSeq), at 3,602,939 to 3,604,246
_3028
Position (kb)
3600
3601
3602
3603 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8
9 at 3600.032 kb on - strand at 3600.111 kb on - strand at 3600.983 kb on - strand at 3600.990 kb on + strand at 3600.991 kb on - strand at 3600.991 kb on - strand at 3600.991 kb on - strand at 3600.991 kb on - strand at 3600.991 kb on - strand at 3600.991 kb on - strand at 3600.991 kb on - strand at 3600.991 kb on - strand at 3600.991 kb on - strand at 3601.035 kb on - strand at 3601.065 kb on - strand at 3601.150 kb on - strand at 3601.156 kb on - strand at 3601.208 kb on - strand, within mutL at 3601.240 kb on + strand, within mutL at 3601.241 kb on - strand, within mutL at 3601.296 kb on + strand, within mutL at 3601.296 kb on + strand, within mutL at 3601.297 kb on - strand, within mutL at 3601.377 kb on + strand, within mutL at 3601.380 kb on + strand, within mutL at 3601.380 kb on + strand, within mutL at 3601.380 kb on + strand, within mutL at 3601.380 kb on + strand, within mutL at 3601.381 kb on - strand, within mutL at 3601.390 kb on - strand, within mutL at 3601.415 kb on + strand, within mutL at 3601.416 kb on - strand, within mutL at 3601.518 kb on + strand, within mutL at 3601.518 kb on + strand, within mutL at 3601.549 kb on + strand, within mutL at 3601.549 kb on + strand, within mutL at 3601.550 kb on - strand, within mutL at 3601.550 kb on - strand, within mutL at 3601.550 kb on - strand, within mutL at 3601.550 kb on - strand, within mutL at 3601.553 kb on - strand, within mutL at 3601.597 kb on + strand, within mutL at 3601.597 kb on + strand, within mutL at 3601.597 kb on + strand, within mutL at 3601.597 kb on + strand, within mutL at 3601.597 kb on + strand, within mutL at 3601.597 kb on + strand, within mutL at 3601.598 kb on - strand, within mutL at 3601.598 kb on - strand, within mutL at 3601.598 kb on - strand, within mutL at 3601.598 kb on - strand, within mutL at 3601.598 kb on - strand, within mutL at 3601.598 kb on - strand, within mutL at 3601.598 kb on - strand, within mutL at 3601.598 kb on - strand, within mutL at 3601.598 kb on - strand, within mutL at 3601.598 kb on - strand, within mutL at 3601.598 kb on - strand, within mutL at 3601.624 kb on + strand, within mutL at 3601.625 kb on - strand, within mutL at 3601.739 kb on + strand, within mutL at 3601.739 kb on + strand, within mutL at 3601.739 kb on + strand, within mutL at 3601.740 kb on - strand, within mutL at 3601.740 kb on - strand, within mutL at 3601.802 kb on + strand, within mutL at 3601.802 kb on + strand, within mutL at 3601.802 kb on + strand, within mutL at 3601.841 kb on + strand, within mutL at 3601.842 kb on - strand, within mutL at 3601.842 kb on - strand, within mutL at 3601.847 kb on + strand, within mutL at 3601.847 kb on + strand, within mutL at 3601.847 kb on + strand, within mutL at 3601.848 kb on - strand, within mutL at 3601.848 kb on - strand, within mutL at 3601.849 kb on + strand, within mutL at 3601.849 kb on + strand, within mutL at 3601.849 kb on + strand, within mutL at 3601.850 kb on - strand, within mutL at 3601.850 kb on - strand, within mutL at 3601.850 kb on - strand, within mutL at 3601.850 kb on - strand, within mutL at 3601.850 kb on - strand, within mutL at 3601.868 kb on + strand, within mutL at 3601.868 kb on + strand, within mutL at 3601.868 kb on + strand, within mutL at 3601.868 kb on + strand, within mutL at 3601.869 kb on - strand, within mutL at 3601.869 kb on - strand, within mutL at 3601.869 kb on - strand, within mutL at 3601.944 kb on + strand, within mutL at 3601.945 kb on - strand, within mutL at 3601.945 kb on - strand, within mutL at 3601.945 kb on - strand, within mutL at 3601.970 kb on + strand, within mutL at 3601.971 kb on - strand, within mutL at 3601.984 kb on + strand, within mutL at 3601.985 kb on - strand, within mutL at 3601.985 kb on - strand, within mutL at 3601.985 kb on - strand, within mutL at 3602.115 kb on + strand, within mutL at 3602.161 kb on + strand, within mutL at 3602.161 kb on + strand, within mutL at 3602.161 kb on + strand, within mutL at 3602.161 kb on + strand, within mutL at 3602.162 kb on - strand, within mutL at 3602.162 kb on - strand, within mutL at 3602.162 kb on - strand, within mutL at 3602.162 kb on - strand, within mutL at 3602.167 kb on + strand, within mutL at 3602.205 kb on + strand, within mutL at 3602.205 kb on + strand, within mutL at 3602.205 kb on + strand, within mutL at 3602.205 kb on + strand, within mutL at 3602.206 kb on - strand, within mutL at 3602.206 kb on - strand, within mutL at 3602.206 kb on - strand, within mutL at 3602.206 kb on - strand, within mutL at 3602.209 kb on + strand, within mutL at 3602.209 kb on + strand, within mutL at 3602.209 kb on + strand, within mutL at 3602.210 kb on - strand, within mutL at 3602.210 kb on - strand, within mutL at 3602.302 kb on + strand, within mutL at 3602.303 kb on - strand, within mutL at 3602.303 kb on - strand, within mutL at 3602.309 kb on + strand, within mutL at 3602.382 kb on + strand, within mutL at 3602.414 kb on + strand, within mutL at 3602.414 kb on + strand, within mutL at 3602.414 kb on + strand, within mutL at 3602.465 kb on + strand, within mutL at 3602.468 kb on + strand, within mutL at 3602.468 kb on + strand, within mutL at 3602.469 kb on - strand, within mutL at 3602.469 kb on - strand, within mutL at 3602.469 kb on - strand, within mutL at 3602.498 kb on + strand, within mutL at 3602.499 kb on - strand, within mutL at 3602.499 kb on - strand, within mutL at 3602.555 kb on - strand, within mutL at 3602.621 kb on + strand, within mutL at 3602.621 kb on + strand, within mutL at 3602.622 kb on - strand, within mutL at 3602.622 kb on - strand, within mutL at 3602.622 kb on - strand, within mutL at 3602.760 kb on - strand at 3602.766 kb on - strand at 3602.796 kb on - strand at 3602.845 kb on - strand at 3602.865 kb on + strand at 3602.866 kb on - strand at 3602.930 kb on - strand at 3602.930 kb on - strand at 3602.989 kb on + strand at 3602.989 kb on + strand at 3602.989 kb on + strand at 3602.989 kb on + strand at 3602.990 kb on - strand at 3602.990 kb on - strand at 3602.990 kb on - strand at 3603.031 kb on - strand at 3603.031 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 3,600,032 - +0.8 3,600,111 - -0.8 3,600,983 - +1.2 3,600,990 + +1.2 3,600,991 - +0.8 3,600,991 - +0.2 3,600,991 - -0.3 3,600,991 - +0.2 3,600,991 - -0.0 3,600,991 - +3.2 3,600,991 - -0.4 3,600,991 - +1.0 3,600,991 - -0.4 3,601,035 - +0.7 3,601,065 - -0.1 3,601,150 - +6.3 3,601,156 - -1.7 3,601,208 - mutL Sama_3027 0.12 +0.3 3,601,240 + mutL Sama_3027 0.14 +2.0 3,601,241 - mutL Sama_3027 0.14 +1.2 3,601,296 + mutL Sama_3027 0.17 -0.2 3,601,296 + mutL Sama_3027 0.17 -0.8 3,601,297 - mutL Sama_3027 0.17 +0.2 3,601,377 + mutL Sama_3027 0.21 +7.6 3,601,380 + mutL Sama_3027 0.21 -0.2 3,601,380 + mutL Sama_3027 0.21 -0.0 3,601,380 + mutL Sama_3027 0.21 -0.0 3,601,380 + mutL Sama_3027 0.21 +0.3 3,601,381 - mutL Sama_3027 0.21 +1.0 3,601,390 - mutL Sama_3027 0.21 -0.0 3,601,415 + mutL Sama_3027 0.23 -0.7 3,601,416 - mutL Sama_3027 0.23 +1.3 3,601,518 + mutL Sama_3027 0.28 -0.7 3,601,518 + mutL Sama_3027 0.28 +1.3 3,601,549 + mutL Sama_3027 0.30 +0.1 3,601,549 + mutL Sama_3027 0.30 +0.6 3,601,550 - mutL Sama_3027 0.30 +0.7 3,601,550 - mutL Sama_3027 0.30 +0.6 3,601,550 - mutL Sama_3027 0.30 +0.7 3,601,550 - mutL Sama_3027 0.30 +1.2 3,601,553 - mutL Sama_3027 0.30 -0.0 3,601,597 + mutL Sama_3027 0.32 -1.8 3,601,597 + mutL Sama_3027 0.32 +0.2 3,601,597 + mutL Sama_3027 0.32 -0.6 3,601,597 + mutL Sama_3027 0.32 +0.6 3,601,597 + mutL Sama_3027 0.32 -1.7 3,601,597 + mutL Sama_3027 0.32 -1.3 3,601,598 - mutL Sama_3027 0.32 +0.6 3,601,598 - mutL Sama_3027 0.32 +0.2 3,601,598 - mutL Sama_3027 0.32 +3.2 3,601,598 - mutL Sama_3027 0.32 -0.3 3,601,598 - mutL Sama_3027 0.32 +0.4 3,601,598 - mutL Sama_3027 0.32 +9.0 3,601,598 - mutL Sama_3027 0.32 +1.4 3,601,598 - mutL Sama_3027 0.32 +0.3 3,601,598 - mutL Sama_3027 0.32 -0.3 3,601,598 - mutL Sama_3027 0.32 +4.7 3,601,598 - mutL Sama_3027 0.32 -1.2 3,601,624 + mutL Sama_3027 0.33 +1.0 3,601,625 - mutL Sama_3027 0.33 +1.6 3,601,739 + mutL Sama_3027 0.39 +0.1 3,601,739 + mutL Sama_3027 0.39 -1.4 3,601,739 + mutL Sama_3027 0.39 -0.3 3,601,740 - mutL Sama_3027 0.39 -0.2 3,601,740 - mutL Sama_3027 0.39 +1.7 3,601,802 + mutL Sama_3027 0.42 +1.0 3,601,802 + mutL Sama_3027 0.42 +0.7 3,601,802 + mutL Sama_3027 0.42 +1.9 3,601,841 + mutL Sama_3027 0.44 +0.5 3,601,842 - mutL Sama_3027 0.45 -0.7 3,601,842 - mutL Sama_3027 0.45 +3.6 3,601,847 + mutL Sama_3027 0.45 -0.5 3,601,847 + mutL Sama_3027 0.45 -2.4 3,601,847 + mutL Sama_3027 0.45 -0.8 3,601,848 - mutL Sama_3027 0.45 +0.9 3,601,848 - mutL Sama_3027 0.45 -0.5 3,601,849 + mutL Sama_3027 0.45 +1.9 3,601,849 + mutL Sama_3027 0.45 -1.5 3,601,849 + mutL Sama_3027 0.45 -0.8 3,601,850 - mutL Sama_3027 0.45 -0.0 3,601,850 - mutL Sama_3027 0.45 -0.3 3,601,850 - mutL Sama_3027 0.45 -1.1 3,601,850 - mutL Sama_3027 0.45 +1.4 3,601,850 - mutL Sama_3027 0.45 +0.2 3,601,868 + mutL Sama_3027 0.46 -0.0 3,601,868 + mutL Sama_3027 0.46 -0.7 3,601,868 + mutL Sama_3027 0.46 +1.0 3,601,868 + mutL Sama_3027 0.46 -0.7 3,601,869 - mutL Sama_3027 0.46 -0.0 3,601,869 - mutL Sama_3027 0.46 +1.6 3,601,869 - mutL Sama_3027 0.46 +1.1 3,601,944 + mutL Sama_3027 0.50 -1.4 3,601,945 - mutL Sama_3027 0.50 +0.6 3,601,945 - mutL Sama_3027 0.50 +3.9 3,601,945 - mutL Sama_3027 0.50 -0.8 3,601,970 + mutL Sama_3027 0.51 -1.1 3,601,971 - mutL Sama_3027 0.51 -1.2 3,601,984 + mutL Sama_3027 0.52 +3.2 3,601,985 - mutL Sama_3027 0.52 +7.7 3,601,985 - mutL Sama_3027 0.52 +3.2 3,601,985 - mutL Sama_3027 0.52 +0.3 3,602,115 + mutL Sama_3027 0.59 +1.4 3,602,161 + mutL Sama_3027 0.61 +3.2 3,602,161 + mutL Sama_3027 0.61 +0.6 3,602,161 + mutL Sama_3027 0.61 +2.6 3,602,161 + mutL Sama_3027 0.61 +1.8 3,602,162 - mutL Sama_3027 0.61 -0.8 3,602,162 - mutL Sama_3027 0.61 +0.6 3,602,162 - mutL Sama_3027 0.61 +0.7 3,602,162 - mutL Sama_3027 0.61 -0.7 3,602,167 + mutL Sama_3027 0.61 +6.7 3,602,205 + mutL Sama_3027 0.63 +2.2 3,602,205 + mutL Sama_3027 0.63 +1.9 3,602,205 + mutL Sama_3027 0.63 +0.6 3,602,205 + mutL Sama_3027 0.63 +0.7 3,602,206 - mutL Sama_3027 0.63 +0.6 3,602,206 - mutL Sama_3027 0.63 +1.3 3,602,206 - mutL Sama_3027 0.63 +2.6 3,602,206 - mutL Sama_3027 0.63 +0.2 3,602,209 + mutL Sama_3027 0.63 -0.5 3,602,209 + mutL Sama_3027 0.63 -0.0 3,602,209 + mutL Sama_3027 0.63 -0.7 3,602,210 - mutL Sama_3027 0.63 +0.8 3,602,210 - mutL Sama_3027 0.63 +0.2 3,602,302 + mutL Sama_3027 0.68 +0.6 3,602,303 - mutL Sama_3027 0.68 +3.2 3,602,303 - mutL Sama_3027 0.68 +0.3 3,602,309 + mutL Sama_3027 0.68 +0.7 3,602,382 + mutL Sama_3027 0.72 -0.7 3,602,414 + mutL Sama_3027 0.74 +1.5 3,602,414 + mutL Sama_3027 0.74 +1.1 3,602,414 + mutL Sama_3027 0.74 -0.5 3,602,465 + mutL Sama_3027 0.76 +0.2 3,602,468 + mutL Sama_3027 0.77 -0.5 3,602,468 + mutL Sama_3027 0.77 +0.2 3,602,469 - mutL Sama_3027 0.77 +0.6 3,602,469 - mutL Sama_3027 0.77 -1.0 3,602,469 - mutL Sama_3027 0.77 +8.3 3,602,498 + mutL Sama_3027 0.78 -1.1 3,602,499 - mutL Sama_3027 0.78 -0.8 3,602,499 - mutL Sama_3027 0.78 -0.7 3,602,555 - mutL Sama_3027 0.81 -0.0 3,602,621 + mutL Sama_3027 0.84 +1.3 3,602,621 + mutL Sama_3027 0.84 +1.2 3,602,622 - mutL Sama_3027 0.84 +0.5 3,602,622 - mutL Sama_3027 0.84 -0.2 3,602,622 - mutL Sama_3027 0.84 +3.1 3,602,760 - +1.9 3,602,766 - +8.3 3,602,796 - +0.8 3,602,845 - +1.4 3,602,865 + -1.7 3,602,866 - +0.2 3,602,930 - +2.0 3,602,930 - -0.3 3,602,989 + -1.4 3,602,989 + -1.2 3,602,989 + +1.3 3,602,989 + -0.5 3,602,990 - +0.3 3,602,990 - -1.5 3,602,990 - +1.7 3,603,031 - +0.1 3,603,031 - +0.5
Or see this region's nucleotide sequence