Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0262 and Sama_0263 are separated by 68 nucleotides Sama_0263 and Sama_0264 are separated by 25 nucleotides Sama_0264 and rpoZ are separated by 24 nucleotides rpoZ and gmk are separated by 77 nucleotides
Sama_0262: Sama_0262 - tRNA guanosine-2'-O-methyltransferase (RefSeq), at 301,630 to 302,355
_0262
Sama_0263: Sama_0263 - YjgF-like protein (RefSeq), at 302,424 to 302,807
_0263
Sama_0264: Sama_0264 - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (RefSeq), at 302,833 to 304,932
_0264
Sama_0265: rpoZ - DNA-directed RNA polymerase subunit omega (RefSeq), at 304,957 to 305,235
rpoZ
Sama_0266: gmk - guanylate kinase (RefSeq), at 305,313 to 305,936
gmk
Position (kb) 302
303
304
305 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8
9
10 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.855 kb on - strand, within Sama_0262 at 301.885 kb on + strand, within Sama_0262 at 301.886 kb on - strand, within Sama_0262 at 301.942 kb on - strand, within Sama_0262 at 302.006 kb on - strand, within Sama_0262 at 302.022 kb on + strand, within Sama_0262 at 302.023 kb on - strand, within Sama_0262 at 302.023 kb on - strand, within Sama_0262 at 302.027 kb on + strand, within Sama_0262 at 302.027 kb on + strand, within Sama_0262 at 302.027 kb on + strand, within Sama_0262 at 302.085 kb on + strand, within Sama_0262 at 302.085 kb on + strand, within Sama_0262 at 302.085 kb on + strand, within Sama_0262 at 302.085 kb on + strand, within Sama_0262 at 302.085 kb on + strand, within Sama_0262 at 302.086 kb on - strand, within Sama_0262 at 302.086 kb on - strand, within Sama_0262 at 302.086 kb on - strand, within Sama_0262 at 302.086 kb on - strand, within Sama_0262 at 302.086 kb on - strand, within Sama_0262 at 302.086 kb on - strand, within Sama_0262 at 302.086 kb on - strand, within Sama_0262 at 302.086 kb on - strand, within Sama_0262 at 302.143 kb on - strand, within Sama_0262 at 302.287 kb on + strand at 302.288 kb on - strand at 302.288 kb on - strand at 302.288 kb on - strand at 302.371 kb on + strand at 302.371 kb on + strand at 302.371 kb on + strand at 302.371 kb on + strand at 302.372 kb on - strand at 302.425 kb on + strand at 302.425 kb on + strand at 302.425 kb on + strand at 302.425 kb on + strand at 302.425 kb on + strand at 302.425 kb on + strand at 302.426 kb on - strand at 302.426 kb on - strand at 302.426 kb on - strand at 302.504 kb on - strand, within Sama_0263 at 302.518 kb on + strand, within Sama_0263 at 302.573 kb on + strand, within Sama_0263 at 302.573 kb on + strand, within Sama_0263 at 302.573 kb on + strand, within Sama_0263 at 302.573 kb on + strand, within Sama_0263 at 302.574 kb on - strand, within Sama_0263 at 302.574 kb on - strand, within Sama_0263 at 302.619 kb on + strand, within Sama_0263 at 302.619 kb on + strand, within Sama_0263 at 302.620 kb on - strand, within Sama_0263 at 302.620 kb on - strand, within Sama_0263 at 302.620 kb on - strand, within Sama_0263 at 302.620 kb on - strand, within Sama_0263 at 302.640 kb on + strand, within Sama_0263 at 302.640 kb on + strand, within Sama_0263 at 302.640 kb on + strand, within Sama_0263 at 302.719 kb on + strand, within Sama_0263 at 302.719 kb on + strand, within Sama_0263 at 302.719 kb on + strand, within Sama_0263 at 302.720 kb on - strand, within Sama_0263 at 302.720 kb on - strand, within Sama_0263 at 302.720 kb on - strand, within Sama_0263 at 302.726 kb on + strand, within Sama_0263 at 302.727 kb on - strand, within Sama_0263 at 302.739 kb on + strand, within Sama_0263 at 302.740 kb on - strand, within Sama_0263 at 302.740 kb on - strand, within Sama_0263 at 302.740 kb on - strand, within Sama_0263 at 302.752 kb on + strand, within Sama_0263 at 302.752 kb on + strand, within Sama_0263 at 302.752 kb on + strand, within Sama_0263 at 302.753 kb on - strand, within Sama_0263 at 302.756 kb on + strand, within Sama_0263 at 302.807 kb on + strand at 302.807 kb on + strand at 302.807 kb on + strand at 302.807 kb on + strand at 302.808 kb on - strand at 302.808 kb on - strand at 302.820 kb on + strand at 302.820 kb on + strand at 302.821 kb on - strand at 302.904 kb on + strand at 302.904 kb on + strand at 302.904 kb on + strand at 302.939 kb on + strand at 302.939 kb on + strand at 302.939 kb on + strand at 302.939 kb on + strand at 302.940 kb on - strand at 302.940 kb on - strand at 302.940 kb on - strand at 302.946 kb on + strand at 302.946 kb on + strand at 302.946 kb on + strand at 302.946 kb on + strand at 302.947 kb on - strand at 302.947 kb on - strand at 302.947 kb on - strand at 302.974 kb on - strand at 303.065 kb on + strand, within Sama_0264 at 303.066 kb on - strand, within Sama_0264 at 303.085 kb on - strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.158 kb on - strand, within Sama_0264 at 303.329 kb on - strand, within Sama_0264 at 303.329 kb on - strand, within Sama_0264 at 303.329 kb on - strand, within Sama_0264 at 303.329 kb on - strand, within Sama_0264 at 303.329 kb on - strand, within Sama_0264 at 303.329 kb on - strand, within Sama_0264 at 303.353 kb on - strand, within Sama_0264 at 303.353 kb on - strand, within Sama_0264 at 303.353 kb on - strand, within Sama_0264 at 303.353 kb on - strand, within Sama_0264 at 303.399 kb on + strand, within Sama_0264 at 303.399 kb on + strand, within Sama_0264 at 303.459 kb on + strand, within Sama_0264 at 303.459 kb on + strand, within Sama_0264 at 303.460 kb on - strand, within Sama_0264 at 303.511 kb on + strand, within Sama_0264 at 303.765 kb on - strand, within Sama_0264 at 304.136 kb on - strand, within Sama_0264 at 304.912 kb on + strand at 304.913 kb on - strand at 304.929 kb on - strand at 304.929 kb on - strand at 304.940 kb on - strand at 304.959 kb on - strand at 304.959 kb on - strand at 305.218 kb on + strand at 305.219 kb on - strand at 305.219 kb on - strand at 305.219 kb on - strand at 305.225 kb on - strand at 305.238 kb on - strand at 305.238 kb on - strand at 305.263 kb on - strand at 305.263 kb on - strand at 305.301 kb on - strand at 305.301 kb on - strand at 305.305 kb on + strand at 305.305 kb on + strand at 305.306 kb on - strand at 305.306 kb on - strand at 305.306 kb on - strand at 305.333 kb on + strand at 305.334 kb on - strand at 305.339 kb on + strand at 305.339 kb on + strand at 305.339 kb on + strand at 305.339 kb on + strand at 305.340 kb on - strand at 305.340 kb on - strand at 305.373 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 301,844 + Sama_0262 0.29 +0.6 301,844 + Sama_0262 0.29 +1.2 301,844 + Sama_0262 0.29 +0.6 301,844 + Sama_0262 0.29 -1.1 301,844 + Sama_0262 0.29 -0.3 301,844 + Sama_0262 0.29 +0.2 301,844 + Sama_0262 0.29 -0.7 301,844 + Sama_0262 0.29 -0.2 301,844 + Sama_0262 0.29 -0.7 301,844 + Sama_0262 0.29 -1.6 301,844 + Sama_0262 0.29 -0.7 301,844 + Sama_0262 0.29 -0.2 301,844 + Sama_0262 0.29 -1.1 301,844 + Sama_0262 0.29 +0.3 301,845 - Sama_0262 0.30 -2.4 301,845 - Sama_0262 0.30 +2.1 301,845 - Sama_0262 0.30 -2.8 301,845 - Sama_0262 0.30 -1.5 301,845 - Sama_0262 0.30 -1.5 301,845 - Sama_0262 0.30 -0.3 301,845 - Sama_0262 0.30 +0.9 301,845 - Sama_0262 0.30 +1.4 301,845 - Sama_0262 0.30 -0.4 301,845 - Sama_0262 0.30 +1.2 301,855 - Sama_0262 0.31 -0.9 301,885 + Sama_0262 0.35 -1.2 301,886 - Sama_0262 0.35 +0.8 301,942 - Sama_0262 0.43 -1.2 302,006 - Sama_0262 0.52 -1.5 302,022 + Sama_0262 0.54 +0.5 302,023 - Sama_0262 0.54 -0.5 302,023 - Sama_0262 0.54 +0.4 302,027 + Sama_0262 0.55 -1.0 302,027 + Sama_0262 0.55 +0.5 302,027 + Sama_0262 0.55 -0.3 302,085 + Sama_0262 0.63 -0.4 302,085 + Sama_0262 0.63 -1.1 302,085 + Sama_0262 0.63 -1.1 302,085 + Sama_0262 0.63 +0.5 302,085 + Sama_0262 0.63 -0.4 302,086 - Sama_0262 0.63 -0.1 302,086 - Sama_0262 0.63 +1.6 302,086 - Sama_0262 0.63 +0.9 302,086 - Sama_0262 0.63 +0.1 302,086 - Sama_0262 0.63 +0.2 302,086 - Sama_0262 0.63 +0.2 302,086 - Sama_0262 0.63 -0.1 302,086 - Sama_0262 0.63 -1.5 302,143 - Sama_0262 0.71 -1.7 302,287 + +3.2 302,288 - -1.8 302,288 - +1.6 302,288 - -0.7 302,371 + -1.5 302,371 + -0.5 302,371 + -1.3 302,371 + -0.7 302,372 - -2.3 302,425 + -0.1 302,425 + +0.2 302,425 + -0.6 302,425 + +2.4 302,425 + -0.2 302,425 + +0.4 302,426 - +0.4 302,426 - -0.8 302,426 - -1.1 302,504 - Sama_0263 0.21 +1.6 302,518 + Sama_0263 0.24 +1.6 302,573 + Sama_0263 0.39 +0.5 302,573 + Sama_0263 0.39 -1.0 302,573 + Sama_0263 0.39 +3.0 302,573 + Sama_0263 0.39 +1.6 302,574 - Sama_0263 0.39 +1.0 302,574 - Sama_0263 0.39 -0.5 302,619 + Sama_0263 0.51 +0.1 302,619 + Sama_0263 0.51 +1.3 302,620 - Sama_0263 0.51 +1.1 302,620 - Sama_0263 0.51 +0.8 302,620 - Sama_0263 0.51 +1.5 302,620 - Sama_0263 0.51 +2.2 302,640 + Sama_0263 0.56 +0.3 302,640 + Sama_0263 0.56 -1.5 302,640 + Sama_0263 0.56 +4.2 302,719 + Sama_0263 0.77 +0.7 302,719 + Sama_0263 0.77 -0.1 302,719 + Sama_0263 0.77 +0.6 302,720 - Sama_0263 0.77 +1.8 302,720 - Sama_0263 0.77 +1.1 302,720 - Sama_0263 0.77 +0.1 302,726 + Sama_0263 0.79 +2.0 302,727 - Sama_0263 0.79 +1.4 302,739 + Sama_0263 0.82 -1.1 302,740 - Sama_0263 0.82 +0.8 302,740 - Sama_0263 0.82 +0.9 302,740 - Sama_0263 0.82 +1.3 302,752 + Sama_0263 0.85 +1.5 302,752 + Sama_0263 0.85 +1.4 302,752 + Sama_0263 0.85 +3.2 302,753 - Sama_0263 0.86 +1.9 302,756 + Sama_0263 0.86 +1.0 302,807 + +1.6 302,807 + +1.2 302,807 + +2.3 302,807 + +1.5 302,808 - +0.9 302,808 - +1.4 302,820 + +1.8 302,820 + +1.1 302,821 - +0.0 302,904 + +4.8 302,904 + +5.6 302,904 + +5.6 302,939 + +2.9 302,939 + +3.6 302,939 + +2.8 302,939 + +1.9 302,940 - +4.4 302,940 - +2.7 302,940 - +3.9 302,946 + +3.5 302,946 + +3.5 302,946 + +4.9 302,946 + +4.2 302,947 - +1.6 302,947 - +4.3 302,947 - +3.3 302,974 - +3.5 303,065 + Sama_0264 0.11 +4.0 303,066 - Sama_0264 0.11 +2.4 303,085 - Sama_0264 0.12 +3.9 303,104 + Sama_0264 0.13 +4.7 303,104 + Sama_0264 0.13 +3.8 303,104 + Sama_0264 0.13 +2.6 303,104 + Sama_0264 0.13 +4.6 303,104 + Sama_0264 0.13 +1.4 303,104 + Sama_0264 0.13 +3.6 303,104 + Sama_0264 0.13 +3.3 303,104 + Sama_0264 0.13 +2.5 303,104 + Sama_0264 0.13 +2.4 303,104 + Sama_0264 0.13 +2.4 303,105 - Sama_0264 0.13 +3.2 303,105 - Sama_0264 0.13 +1.3 303,105 - Sama_0264 0.13 +3.0 303,105 - Sama_0264 0.13 +2.4 303,105 - Sama_0264 0.13 +2.0 303,105 - Sama_0264 0.13 +3.6 303,105 - Sama_0264 0.13 +2.0 303,105 - Sama_0264 0.13 +1.9 303,105 - Sama_0264 0.13 +2.9 303,105 - Sama_0264 0.13 +3.5 303,105 - Sama_0264 0.13 +3.5 303,158 - Sama_0264 0.15 +5.3 303,329 - Sama_0264 0.24 +2.4 303,329 - Sama_0264 0.24 +4.1 303,329 - Sama_0264 0.24 +4.9 303,329 - Sama_0264 0.24 +4.2 303,329 - Sama_0264 0.24 +3.3 303,329 - Sama_0264 0.24 +3.1 303,353 - Sama_0264 0.25 +3.7 303,353 - Sama_0264 0.25 +0.2 303,353 - Sama_0264 0.25 +2.3 303,353 - Sama_0264 0.25 +3.1 303,399 + Sama_0264 0.27 +5.7 303,399 + Sama_0264 0.27 +5.9 303,459 + Sama_0264 0.30 +9.7 303,459 + Sama_0264 0.30 +8.3 303,460 - Sama_0264 0.30 +2.3 303,511 + Sama_0264 0.32 +7.8 303,765 - Sama_0264 0.44 +9.0 304,136 - Sama_0264 0.62 +0.4 304,912 + +8.0 304,913 - +3.9 304,929 - +6.5 304,929 - +5.1 304,940 - +4.0 304,959 - +1.3 304,959 - +1.8 305,218 + +10.1 305,219 - -0.1 305,219 - -0.3 305,219 - +0.9 305,225 - -0.6 305,238 - -0.3 305,238 - -0.0 305,263 - -0.1 305,263 - +1.3 305,301 - -0.1 305,301 - +0.9 305,305 + +6.9 305,305 + +8.0 305,306 - -0.6 305,306 - +1.2 305,306 - -0.4 305,333 + +1.2 305,334 - -1.0 305,339 + +0.6 305,339 + +2.2 305,339 + -2.2 305,339 + -1.2 305,340 - +0.1 305,340 - -1.3 305,373 + +2.0
Or see this region's nucleotide sequence