Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0264 and rpoZ are separated by 24 nucleotides rpoZ and gmk are separated by 77 nucleotides gmk and Sama_0267 are separated by 187 nucleotides
Sama_0264: Sama_0264 - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (RefSeq), at 302,833 to 304,932
_0264
Sama_0265: rpoZ - DNA-directed RNA polymerase subunit omega (RefSeq), at 304,957 to 305,235
rpoZ
Sama_0266: gmk - guanylate kinase (RefSeq), at 305,313 to 305,936
gmk
Sama_0267: Sama_0267 - hypothetical protein (RefSeq), at 306,124 to 307,608
_0267
Position (kb) 305
306 Strain fitness (log2 ratio) -2
-1
0
1
2 at 304.912 kb on + strand at 304.913 kb on - strand at 304.929 kb on - strand at 304.929 kb on - strand at 304.940 kb on - strand at 304.959 kb on - strand at 304.959 kb on - strand at 305.218 kb on + strand at 305.219 kb on - strand at 305.219 kb on - strand at 305.219 kb on - strand at 305.225 kb on - strand at 305.238 kb on - strand at 305.238 kb on - strand at 305.263 kb on - strand at 305.263 kb on - strand at 305.301 kb on - strand at 305.301 kb on - strand at 305.305 kb on + strand at 305.305 kb on + strand at 305.306 kb on - strand at 305.306 kb on - strand at 305.306 kb on - strand at 305.333 kb on + strand at 305.334 kb on - strand at 305.339 kb on + strand at 305.339 kb on + strand at 305.339 kb on + strand at 305.339 kb on + strand at 305.340 kb on - strand at 305.340 kb on - strand at 305.373 kb on + strand at 306.001 kb on - strand at 306.001 kb on - strand at 306.013 kb on - strand at 306.013 kb on - strand at 306.015 kb on - strand at 306.015 kb on - strand at 306.081 kb on - strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.095 kb on + strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.096 kb on - strand at 306.097 kb on + strand at 306.097 kb on + strand at 306.097 kb on + strand at 306.097 kb on + strand at 306.097 kb on + strand at 306.097 kb on + strand at 306.097 kb on + strand at 306.097 kb on + strand at 306.097 kb on + strand at 306.098 kb on - strand at 306.098 kb on - strand at 306.098 kb on - strand at 306.098 kb on - strand at 306.098 kb on - strand at 306.145 kb on - strand at 306.145 kb on - strand at 306.175 kb on - strand at 306.200 kb on - strand at 306.200 kb on - strand at 306.200 kb on - strand at 306.200 kb on - strand at 306.332 kb on - strand, within Sama_0267 at 306.372 kb on - strand, within Sama_0267 at 306.382 kb on - strand, within Sama_0267 at 306.382 kb on - strand, within Sama_0267 at 306.432 kb on + strand, within Sama_0267 at 306.432 kb on + strand, within Sama_0267 at 306.432 kb on + strand, within Sama_0267 at 306.432 kb on + strand, within Sama_0267 at 306.432 kb on + strand, within Sama_0267 at 306.432 kb on + strand, within Sama_0267 at 306.433 kb on - strand, within Sama_0267 at 306.478 kb on + strand, within Sama_0267 at 306.683 kb on + strand, within Sama_0267 at 306.730 kb on + strand, within Sama_0267 at 306.730 kb on + strand, within Sama_0267 at 306.936 kb on + strand, within Sama_0267
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 304,912 + +0.7 304,913 - +1.8 304,929 - +0.7 304,929 - +0.4 304,940 - +1.2 304,959 - +0.8 304,959 - +0.4 305,218 + +0.4 305,219 - +0.9 305,219 - +1.6 305,219 - +0.4 305,225 - +1.0 305,238 - -2.1 305,238 - +0.7 305,263 - -0.6 305,263 - +0.7 305,301 - -1.9 305,301 - -0.9 305,305 + +1.4 305,305 + +1.6 305,306 - -0.5 305,306 - +0.6 305,306 - +0.1 305,333 + +1.6 305,334 - +0.5 305,339 + -0.2 305,339 + -0.6 305,339 + -1.0 305,339 + +0.7 305,340 - +0.0 305,340 - -0.3 305,373 + -0.3 306,001 - -0.1 306,001 - +0.7 306,013 - -0.6 306,013 - +1.4 306,015 - -0.0 306,015 - -0.3 306,081 - -0.2 306,095 + -0.2 306,095 + +0.2 306,095 + -0.4 306,095 + +0.1 306,095 + +0.5 306,095 + +0.3 306,095 + +1.1 306,095 + -0.0 306,095 + +0.1 306,095 + -0.1 306,095 + +1.1 306,096 - -0.0 306,096 - -0.2 306,096 - -2.1 306,096 - -0.3 306,096 - +1.7 306,096 - +0.9 306,096 - -0.4 306,096 - -2.6 306,096 - -0.2 306,096 - -0.8 306,096 - -0.2 306,097 + -0.6 306,097 + -0.2 306,097 + -0.4 306,097 + -1.3 306,097 + +0.1 306,097 + -1.3 306,097 + +1.4 306,097 + +0.1 306,097 + -0.2 306,098 - +0.8 306,098 - -0.0 306,098 - -1.2 306,098 - +0.7 306,098 - +0.1 306,145 - -0.6 306,145 - +1.7 306,175 - -0.4 306,200 - +0.1 306,200 - -0.6 306,200 - -2.1 306,200 - +0.8 306,332 - Sama_0267 0.14 +0.5 306,372 - Sama_0267 0.17 -1.1 306,382 - Sama_0267 0.17 -2.7 306,382 - Sama_0267 0.17 -0.4 306,432 + Sama_0267 0.21 +0.7 306,432 + Sama_0267 0.21 -0.2 306,432 + Sama_0267 0.21 -2.4 306,432 + Sama_0267 0.21 -1.4 306,432 + Sama_0267 0.21 -0.1 306,432 + Sama_0267 0.21 -0.5 306,433 - Sama_0267 0.21 +0.0 306,478 + Sama_0267 0.24 +1.5 306,683 + Sama_0267 0.38 -1.0 306,730 + Sama_0267 0.41 -0.4 306,730 + Sama_0267 0.41 -0.2 306,936 + Sama_0267 0.55 -0.9
Or see this region's nucleotide sequence