Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0262 and Sama_0263 are separated by 68 nucleotides Sama_0263 and Sama_0264 are separated by 25 nucleotides Sama_0264 and rpoZ are separated by 24 nucleotides rpoZ and gmk are separated by 77 nucleotides
Sama_0262: Sama_0262 - tRNA guanosine-2'-O-methyltransferase (RefSeq), at 301,630 to 302,355
_0262
Sama_0263: Sama_0263 - YjgF-like protein (RefSeq), at 302,424 to 302,807
_0263
Sama_0264: Sama_0264 - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (RefSeq), at 302,833 to 304,932
_0264
Sama_0265: rpoZ - DNA-directed RNA polymerase subunit omega (RefSeq), at 304,957 to 305,235
rpoZ
Sama_0266: gmk - guanylate kinase (RefSeq), at 305,313 to 305,936
gmk
Position (kb) 302
303
304
305 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.844 kb on + strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.845 kb on - strand, within Sama_0262 at 301.855 kb on - strand, within Sama_0262 at 301.885 kb on + strand, within Sama_0262 at 301.886 kb on - strand, within Sama_0262 at 301.942 kb on - strand, within Sama_0262 at 302.006 kb on - strand, within Sama_0262 at 302.022 kb on + strand, within Sama_0262 at 302.023 kb on - strand, within Sama_0262 at 302.023 kb on - strand, within Sama_0262 at 302.027 kb on + strand, within Sama_0262 at 302.027 kb on + strand, within Sama_0262 at 302.027 kb on + strand, within Sama_0262 at 302.085 kb on + strand, within Sama_0262 at 302.085 kb on + strand, within Sama_0262 at 302.085 kb on + strand, within Sama_0262 at 302.085 kb on + strand, within Sama_0262 at 302.085 kb on + strand, within Sama_0262 at 302.086 kb on - strand, within Sama_0262 at 302.086 kb on - strand, within Sama_0262 at 302.086 kb on - strand, within Sama_0262 at 302.086 kb on - strand, within Sama_0262 at 302.086 kb on - strand, within Sama_0262 at 302.086 kb on - strand, within Sama_0262 at 302.086 kb on - strand, within Sama_0262 at 302.086 kb on - strand, within Sama_0262 at 302.143 kb on - strand, within Sama_0262 at 302.287 kb on + strand at 302.288 kb on - strand at 302.288 kb on - strand at 302.288 kb on - strand at 302.371 kb on + strand at 302.371 kb on + strand at 302.371 kb on + strand at 302.371 kb on + strand at 302.372 kb on - strand at 302.425 kb on + strand at 302.425 kb on + strand at 302.425 kb on + strand at 302.425 kb on + strand at 302.425 kb on + strand at 302.425 kb on + strand at 302.426 kb on - strand at 302.426 kb on - strand at 302.426 kb on - strand at 302.504 kb on - strand, within Sama_0263 at 302.518 kb on + strand, within Sama_0263 at 302.573 kb on + strand, within Sama_0263 at 302.573 kb on + strand, within Sama_0263 at 302.573 kb on + strand, within Sama_0263 at 302.573 kb on + strand, within Sama_0263 at 302.574 kb on - strand, within Sama_0263 at 302.574 kb on - strand, within Sama_0263 at 302.619 kb on + strand, within Sama_0263 at 302.619 kb on + strand, within Sama_0263 at 302.620 kb on - strand, within Sama_0263 at 302.620 kb on - strand, within Sama_0263 at 302.620 kb on - strand, within Sama_0263 at 302.620 kb on - strand, within Sama_0263 at 302.640 kb on + strand, within Sama_0263 at 302.640 kb on + strand, within Sama_0263 at 302.640 kb on + strand, within Sama_0263 at 302.719 kb on + strand, within Sama_0263 at 302.719 kb on + strand, within Sama_0263 at 302.719 kb on + strand, within Sama_0263 at 302.720 kb on - strand, within Sama_0263 at 302.720 kb on - strand, within Sama_0263 at 302.720 kb on - strand, within Sama_0263 at 302.726 kb on + strand, within Sama_0263 at 302.727 kb on - strand, within Sama_0263 at 302.739 kb on + strand, within Sama_0263 at 302.740 kb on - strand, within Sama_0263 at 302.740 kb on - strand, within Sama_0263 at 302.740 kb on - strand, within Sama_0263 at 302.752 kb on + strand, within Sama_0263 at 302.752 kb on + strand, within Sama_0263 at 302.752 kb on + strand, within Sama_0263 at 302.753 kb on - strand, within Sama_0263 at 302.756 kb on + strand, within Sama_0263 at 302.807 kb on + strand at 302.807 kb on + strand at 302.807 kb on + strand at 302.807 kb on + strand at 302.808 kb on - strand at 302.808 kb on - strand at 302.820 kb on + strand at 302.820 kb on + strand at 302.821 kb on - strand at 302.904 kb on + strand at 302.904 kb on + strand at 302.904 kb on + strand at 302.939 kb on + strand at 302.939 kb on + strand at 302.939 kb on + strand at 302.939 kb on + strand at 302.940 kb on - strand at 302.940 kb on - strand at 302.940 kb on - strand at 302.946 kb on + strand at 302.946 kb on + strand at 302.946 kb on + strand at 302.946 kb on + strand at 302.947 kb on - strand at 302.947 kb on - strand at 302.947 kb on - strand at 302.974 kb on - strand at 303.065 kb on + strand, within Sama_0264 at 303.066 kb on - strand, within Sama_0264 at 303.085 kb on - strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.104 kb on + strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.105 kb on - strand, within Sama_0264 at 303.158 kb on - strand, within Sama_0264 at 303.329 kb on - strand, within Sama_0264 at 303.329 kb on - strand, within Sama_0264 at 303.329 kb on - strand, within Sama_0264 at 303.329 kb on - strand, within Sama_0264 at 303.329 kb on - strand, within Sama_0264 at 303.329 kb on - strand, within Sama_0264 at 303.353 kb on - strand, within Sama_0264 at 303.353 kb on - strand, within Sama_0264 at 303.353 kb on - strand, within Sama_0264 at 303.353 kb on - strand, within Sama_0264 at 303.399 kb on + strand, within Sama_0264 at 303.399 kb on + strand, within Sama_0264 at 303.459 kb on + strand, within Sama_0264 at 303.459 kb on + strand, within Sama_0264 at 303.460 kb on - strand, within Sama_0264 at 303.511 kb on + strand, within Sama_0264 at 303.765 kb on - strand, within Sama_0264 at 304.136 kb on - strand, within Sama_0264 at 304.912 kb on + strand at 304.913 kb on - strand at 304.929 kb on - strand at 304.929 kb on - strand at 304.940 kb on - strand at 304.959 kb on - strand at 304.959 kb on - strand at 305.218 kb on + strand at 305.219 kb on - strand at 305.219 kb on - strand at 305.219 kb on - strand at 305.225 kb on - strand at 305.238 kb on - strand at 305.238 kb on - strand at 305.263 kb on - strand at 305.263 kb on - strand at 305.301 kb on - strand at 305.301 kb on - strand at 305.305 kb on + strand at 305.305 kb on + strand at 305.306 kb on - strand at 305.306 kb on - strand at 305.306 kb on - strand at 305.333 kb on + strand at 305.334 kb on - strand at 305.339 kb on + strand at 305.339 kb on + strand at 305.339 kb on + strand at 305.339 kb on + strand at 305.340 kb on - strand at 305.340 kb on - strand at 305.373 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 301,844 + Sama_0262 0.29 +1.6 301,844 + Sama_0262 0.29 -0.6 301,844 + Sama_0262 0.29 -0.2 301,844 + Sama_0262 0.29 +0.5 301,844 + Sama_0262 0.29 -0.5 301,844 + Sama_0262 0.29 +1.4 301,844 + Sama_0262 0.29 -0.9 301,844 + Sama_0262 0.29 -0.4 301,844 + Sama_0262 0.29 -0.5 301,844 + Sama_0262 0.29 -1.3 301,844 + Sama_0262 0.29 -0.2 301,844 + Sama_0262 0.29 +0.2 301,844 + Sama_0262 0.29 +0.4 301,844 + Sama_0262 0.29 +0.5 301,845 - Sama_0262 0.30 -0.3 301,845 - Sama_0262 0.30 -0.2 301,845 - Sama_0262 0.30 +0.5 301,845 - Sama_0262 0.30 +0.7 301,845 - Sama_0262 0.30 -0.9 301,845 - Sama_0262 0.30 +0.6 301,845 - Sama_0262 0.30 -0.7 301,845 - Sama_0262 0.30 +1.2 301,845 - Sama_0262 0.30 +0.1 301,845 - Sama_0262 0.30 -0.6 301,855 - Sama_0262 0.31 +1.1 301,885 + Sama_0262 0.35 +0.2 301,886 - Sama_0262 0.35 -2.0 301,942 - Sama_0262 0.43 +0.2 302,006 - Sama_0262 0.52 -0.3 302,022 + Sama_0262 0.54 +0.6 302,023 - Sama_0262 0.54 +1.3 302,023 - Sama_0262 0.54 +0.2 302,027 + Sama_0262 0.55 -2.8 302,027 + Sama_0262 0.55 +0.4 302,027 + Sama_0262 0.55 -0.8 302,085 + Sama_0262 0.63 +1.1 302,085 + Sama_0262 0.63 +0.1 302,085 + Sama_0262 0.63 -0.1 302,085 + Sama_0262 0.63 -0.7 302,085 + Sama_0262 0.63 -0.6 302,086 - Sama_0262 0.63 +0.7 302,086 - Sama_0262 0.63 -0.2 302,086 - Sama_0262 0.63 -0.9 302,086 - Sama_0262 0.63 -0.7 302,086 - Sama_0262 0.63 -1.6 302,086 - Sama_0262 0.63 +0.4 302,086 - Sama_0262 0.63 -0.4 302,086 - Sama_0262 0.63 +0.9 302,143 - Sama_0262 0.71 +0.1 302,287 + +1.4 302,288 - +0.5 302,288 - +0.6 302,288 - +0.8 302,371 + -0.9 302,371 + -0.6 302,371 + -0.5 302,371 + +0.2 302,372 - -0.5 302,425 + -0.6 302,425 + -2.6 302,425 + -2.4 302,425 + +1.4 302,425 + -0.4 302,425 + -0.4 302,426 - +0.1 302,426 - +0.2 302,426 - +0.3 302,504 - Sama_0263 0.21 +0.5 302,518 + Sama_0263 0.24 +0.8 302,573 + Sama_0263 0.39 -0.6 302,573 + Sama_0263 0.39 +1.7 302,573 + Sama_0263 0.39 +0.4 302,573 + Sama_0263 0.39 +0.3 302,574 - Sama_0263 0.39 +0.5 302,574 - Sama_0263 0.39 +0.7 302,619 + Sama_0263 0.51 -1.3 302,619 + Sama_0263 0.51 -0.3 302,620 - Sama_0263 0.51 -1.1 302,620 - Sama_0263 0.51 +0.8 302,620 - Sama_0263 0.51 -0.8 302,620 - Sama_0263 0.51 +0.6 302,640 + Sama_0263 0.56 +0.0 302,640 + Sama_0263 0.56 -1.3 302,640 + Sama_0263 0.56 +1.4 302,719 + Sama_0263 0.77 -2.1 302,719 + Sama_0263 0.77 -0.4 302,719 + Sama_0263 0.77 +0.4 302,720 - Sama_0263 0.77 -0.7 302,720 - Sama_0263 0.77 -0.2 302,720 - Sama_0263 0.77 +0.6 302,726 + Sama_0263 0.79 -0.8 302,727 - Sama_0263 0.79 +0.2 302,739 + Sama_0263 0.82 +0.2 302,740 - Sama_0263 0.82 +0.2 302,740 - Sama_0263 0.82 -0.9 302,740 - Sama_0263 0.82 +0.5 302,752 + Sama_0263 0.85 -0.3 302,752 + Sama_0263 0.85 -1.9 302,752 + Sama_0263 0.85 +2.6 302,753 - Sama_0263 0.86 +1.5 302,756 + Sama_0263 0.86 +0.5 302,807 + -0.2 302,807 + -0.6 302,807 + -0.1 302,807 + -0.3 302,808 - +0.1 302,808 - +0.3 302,820 + +0.4 302,820 + +0.9 302,821 - -1.1 302,904 + +1.5 302,904 + +1.8 302,904 + +2.2 302,939 + +1.5 302,939 + +0.9 302,939 + +1.7 302,939 + +1.4 302,940 - +1.8 302,940 - +0.8 302,940 - +1.9 302,946 + +2.7 302,946 + -0.1 302,946 + +1.0 302,946 + -0.6 302,947 - +1.4 302,947 - +2.0 302,947 - +1.5 302,974 - +2.5 303,065 + Sama_0264 0.11 +1.2 303,066 - Sama_0264 0.11 +0.2 303,085 - Sama_0264 0.12 +0.6 303,104 + Sama_0264 0.13 +1.8 303,104 + Sama_0264 0.13 +2.0 303,104 + Sama_0264 0.13 +0.9 303,104 + Sama_0264 0.13 +1.0 303,104 + Sama_0264 0.13 +1.6 303,104 + Sama_0264 0.13 +1.4 303,104 + Sama_0264 0.13 +0.1 303,104 + Sama_0264 0.13 +0.8 303,104 + Sama_0264 0.13 +2.6 303,104 + Sama_0264 0.13 +1.0 303,105 - Sama_0264 0.13 +2.4 303,105 - Sama_0264 0.13 +1.6 303,105 - Sama_0264 0.13 +2.4 303,105 - Sama_0264 0.13 -0.2 303,105 - Sama_0264 0.13 +1.7 303,105 - Sama_0264 0.13 +1.0 303,105 - Sama_0264 0.13 +1.6 303,105 - Sama_0264 0.13 +0.8 303,105 - Sama_0264 0.13 +1.1 303,105 - Sama_0264 0.13 +2.7 303,105 - Sama_0264 0.13 +0.5 303,158 - Sama_0264 0.15 +2.0 303,329 - Sama_0264 0.24 +1.0 303,329 - Sama_0264 0.24 +1.3 303,329 - Sama_0264 0.24 +2.0 303,329 - Sama_0264 0.24 +1.3 303,329 - Sama_0264 0.24 +0.3 303,329 - Sama_0264 0.24 +1.8 303,353 - Sama_0264 0.25 +0.9 303,353 - Sama_0264 0.25 -1.6 303,353 - Sama_0264 0.25 +0.8 303,353 - Sama_0264 0.25 +1.9 303,399 + Sama_0264 0.27 +0.2 303,399 + Sama_0264 0.27 -0.3 303,459 + Sama_0264 0.30 +0.8 303,459 + Sama_0264 0.30 -2.1 303,460 - Sama_0264 0.30 +1.5 303,511 + Sama_0264 0.32 -0.9 303,765 - Sama_0264 0.44 -1.6 304,136 - Sama_0264 0.62 -1.4 304,912 + +0.7 304,913 - +1.8 304,929 - +0.7 304,929 - +0.4 304,940 - +1.2 304,959 - +0.8 304,959 - +0.4 305,218 + +0.4 305,219 - +0.9 305,219 - +1.6 305,219 - +0.4 305,225 - +1.0 305,238 - -2.1 305,238 - +0.7 305,263 - -0.6 305,263 - +0.7 305,301 - -1.9 305,301 - -0.9 305,305 + +1.4 305,305 + +1.6 305,306 - -0.5 305,306 - +0.6 305,306 - +0.1 305,333 + +1.6 305,334 - +0.5 305,339 + -0.2 305,339 + -0.6 305,339 + -1.0 305,339 + +0.7 305,340 - +0.0 305,340 - -0.3 305,373 + -0.3
Or see this region's nucleotide sequence