Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0193 and Sama_0194 are separated by 122 nucleotides Sama_0194 and Sama_0195 are separated by 8 nucleotides Sama_0195 and Sama_R0004 are separated by 558 nucleotides
Sama_0193: Sama_0193 - tRNA (uracil-5-)-methyltransferase (RefSeq), at 221,311 to 222,408
_0193
Sama_0194: Sama_0194 - glutamate racemase (RefSeq), at 222,531 to 223,331
_0194
Sama_0195: Sama_0195 - RNA-binding protein (RefSeq), at 223,340 to 223,795
_0195
Sama_R0004: Sama_R0004 - 16S ribosomal RNA (RefSeq), at 224,354 to 225,890
_R0004
Position (kb) 223
224 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 222.377 kb on + strand at 222.377 kb on + strand at 222.378 kb on - strand at 222.378 kb on - strand at 222.378 kb on - strand at 222.378 kb on - strand at 222.427 kb on + strand at 222.447 kb on + strand at 222.463 kb on + strand at 222.463 kb on + strand at 222.463 kb on + strand at 222.463 kb on + strand at 222.463 kb on + strand at 222.463 kb on + strand at 222.463 kb on + strand at 222.463 kb on + strand at 222.463 kb on + strand at 222.464 kb on - strand at 222.464 kb on - strand at 222.464 kb on - strand at 222.464 kb on - strand at 222.464 kb on - strand at 222.464 kb on - strand at 222.464 kb on - strand at 222.464 kb on - strand at 222.490 kb on + strand at 222.490 kb on + strand at 222.495 kb on + strand at 222.495 kb on + strand at 222.495 kb on + strand at 223.312 kb on + strand at 223.313 kb on - strand at 223.313 kb on - strand at 223.313 kb on - strand at 223.322 kb on + strand at 223.322 kb on + strand at 223.323 kb on - strand at 223.323 kb on - strand at 223.323 kb on - strand at 223.389 kb on - strand, within Sama_0195 at 223.389 kb on - strand, within Sama_0195 at 223.394 kb on + strand, within Sama_0195 at 223.394 kb on + strand, within Sama_0195 at 223.394 kb on + strand, within Sama_0195 at 223.394 kb on + strand, within Sama_0195 at 223.395 kb on - strand, within Sama_0195 at 223.395 kb on - strand, within Sama_0195 at 223.395 kb on - strand, within Sama_0195 at 223.416 kb on + strand, within Sama_0195 at 223.416 kb on + strand, within Sama_0195 at 223.416 kb on + strand, within Sama_0195 at 223.417 kb on - strand, within Sama_0195 at 223.417 kb on - strand, within Sama_0195 at 223.525 kb on + strand, within Sama_0195 at 223.537 kb on - strand, within Sama_0195 at 223.556 kb on + strand, within Sama_0195 at 223.556 kb on + strand, within Sama_0195 at 223.556 kb on + strand, within Sama_0195 at 223.556 kb on + strand, within Sama_0195 at 223.556 kb on + strand, within Sama_0195 at 223.557 kb on - strand, within Sama_0195 at 223.557 kb on - strand, within Sama_0195 at 223.557 kb on - strand, within Sama_0195 at 223.557 kb on - strand, within Sama_0195 at 223.557 kb on - strand, within Sama_0195 at 223.557 kb on - strand, within Sama_0195 at 223.557 kb on - strand, within Sama_0195 at 223.596 kb on + strand, within Sama_0195 at 223.596 kb on + strand, within Sama_0195 at 223.596 kb on + strand, within Sama_0195 at 223.596 kb on + strand, within Sama_0195 at 223.596 kb on + strand, within Sama_0195 at 223.596 kb on + strand, within Sama_0195 at 223.596 kb on + strand, within Sama_0195 at 223.597 kb on - strand, within Sama_0195 at 223.597 kb on - strand, within Sama_0195 at 223.597 kb on - strand, within Sama_0195 at 223.597 kb on - strand, within Sama_0195 at 223.597 kb on - strand, within Sama_0195 at 223.618 kb on + strand, within Sama_0195 at 223.618 kb on + strand, within Sama_0195 at 223.618 kb on + strand, within Sama_0195 at 223.620 kb on + strand, within Sama_0195 at 223.620 kb on + strand, within Sama_0195 at 223.620 kb on + strand, within Sama_0195 at 223.620 kb on + strand, within Sama_0195 at 223.620 kb on + strand, within Sama_0195 at 223.620 kb on + strand, within Sama_0195 at 223.620 kb on + strand, within Sama_0195 at 223.621 kb on - strand, within Sama_0195 at 223.621 kb on - strand, within Sama_0195 at 223.621 kb on - strand, within Sama_0195 at 223.621 kb on - strand, within Sama_0195 at 223.621 kb on - strand, within Sama_0195 at 223.621 kb on - strand, within Sama_0195 at 223.621 kb on - strand, within Sama_0195 at 223.621 kb on - strand, within Sama_0195 at 223.672 kb on + strand, within Sama_0195 at 223.672 kb on + strand, within Sama_0195 at 223.673 kb on - strand, within Sama_0195 at 223.673 kb on - strand, within Sama_0195 at 223.673 kb on - strand, within Sama_0195 at 223.676 kb on + strand, within Sama_0195 at 223.676 kb on + strand, within Sama_0195 at 223.676 kb on + strand, within Sama_0195 at 223.676 kb on + strand, within Sama_0195 at 223.677 kb on - strand, within Sama_0195 at 223.677 kb on - strand, within Sama_0195 at 223.694 kb on + strand, within Sama_0195 at 223.694 kb on + strand, within Sama_0195 at 223.694 kb on + strand, within Sama_0195 at 223.694 kb on + strand, within Sama_0195 at 223.695 kb on - strand, within Sama_0195 at 223.695 kb on - strand, within Sama_0195 at 223.695 kb on - strand, within Sama_0195 at 223.699 kb on + strand, within Sama_0195 at 223.700 kb on - strand, within Sama_0195 at 223.710 kb on + strand, within Sama_0195 at 223.710 kb on + strand, within Sama_0195 at 223.710 kb on + strand, within Sama_0195 at 223.710 kb on + strand, within Sama_0195 at 223.727 kb on + strand, within Sama_0195 at 223.727 kb on + strand, within Sama_0195 at 223.728 kb on - strand, within Sama_0195 at 223.728 kb on - strand, within Sama_0195 at 223.777 kb on + strand at 223.778 kb on - strand at 223.820 kb on - strand at 223.831 kb on - strand at 223.842 kb on - strand at 223.851 kb on + strand at 223.851 kb on + strand at 223.852 kb on - strand at 223.853 kb on + strand at 223.853 kb on + strand at 223.853 kb on + strand at 223.853 kb on + strand at 223.854 kb on - strand at 223.854 kb on - strand at 223.854 kb on - strand at 223.855 kb on + strand at 223.855 kb on + strand at 223.855 kb on + strand at 223.855 kb on + strand at 223.855 kb on + strand at 223.855 kb on + strand at 223.855 kb on + strand at 223.855 kb on + strand at 223.856 kb on - strand at 223.856 kb on - strand at 223.856 kb on - strand at 223.856 kb on - strand at 223.856 kb on - strand at 223.856 kb on - strand at 223.856 kb on - strand at 223.856 kb on - strand at 223.856 kb on - strand at 223.856 kb on - strand at 223.856 kb on - strand at 223.859 kb on - strand at 223.876 kb on + strand at 223.877 kb on - strand at 223.934 kb on - strand at 224.005 kb on - strand at 224.061 kb on + strand at 224.062 kb on - strand at 224.062 kb on - strand at 224.063 kb on + strand at 224.063 kb on + strand at 224.063 kb on + strand at 224.063 kb on + strand at 224.064 kb on - strand at 224.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 222,377 + -0.5 222,377 + -2.6 222,378 - +0.4 222,378 - -1.1 222,378 - -0.2 222,378 - -0.7 222,427 + -0.1 222,447 + +0.9 222,463 + +0.2 222,463 + -1.2 222,463 + -1.2 222,463 + +1.1 222,463 + +0.4 222,463 + +0.5 222,463 + +0.6 222,463 + -0.6 222,463 + +0.2 222,464 - +1.4 222,464 - -0.2 222,464 - -3.6 222,464 - +0.6 222,464 - +0.9 222,464 - +0.6 222,464 - +0.3 222,464 - -1.3 222,490 + -0.9 222,490 + -0.0 222,495 + +1.5 222,495 + -0.9 222,495 + +1.3 223,312 + +0.2 223,313 - +1.0 223,313 - -0.5 223,313 - +0.4 223,322 + +1.7 223,322 + -0.8 223,323 - -1.4 223,323 - +1.1 223,323 - -1.4 223,389 - Sama_0195 0.11 +0.7 223,389 - Sama_0195 0.11 +0.5 223,394 + Sama_0195 0.12 +1.0 223,394 + Sama_0195 0.12 +0.8 223,394 + Sama_0195 0.12 +1.1 223,394 + Sama_0195 0.12 +1.4 223,395 - Sama_0195 0.12 -0.5 223,395 - Sama_0195 0.12 -0.1 223,395 - Sama_0195 0.12 +0.9 223,416 + Sama_0195 0.17 -0.9 223,416 + Sama_0195 0.17 +0.0 223,416 + Sama_0195 0.17 +2.2 223,417 - Sama_0195 0.17 -0.3 223,417 - Sama_0195 0.17 +0.5 223,525 + Sama_0195 0.41 +0.6 223,537 - Sama_0195 0.43 +1.1 223,556 + Sama_0195 0.47 +1.4 223,556 + Sama_0195 0.47 +0.2 223,556 + Sama_0195 0.47 -0.8 223,556 + Sama_0195 0.47 +1.7 223,556 + Sama_0195 0.47 +0.4 223,557 - Sama_0195 0.48 +0.8 223,557 - Sama_0195 0.48 +2.4 223,557 - Sama_0195 0.48 -0.3 223,557 - Sama_0195 0.48 -0.0 223,557 - Sama_0195 0.48 +0.0 223,557 - Sama_0195 0.48 +1.2 223,557 - Sama_0195 0.48 +1.0 223,596 + Sama_0195 0.56 +0.8 223,596 + Sama_0195 0.56 -0.7 223,596 + Sama_0195 0.56 -0.0 223,596 + Sama_0195 0.56 +0.2 223,596 + Sama_0195 0.56 +0.6 223,596 + Sama_0195 0.56 +1.2 223,596 + Sama_0195 0.56 +1.0 223,597 - Sama_0195 0.56 -0.9 223,597 - Sama_0195 0.56 +0.4 223,597 - Sama_0195 0.56 +0.7 223,597 - Sama_0195 0.56 +0.1 223,597 - Sama_0195 0.56 +0.8 223,618 + Sama_0195 0.61 -1.0 223,618 + Sama_0195 0.61 +0.4 223,618 + Sama_0195 0.61 +0.7 223,620 + Sama_0195 0.61 +1.7 223,620 + Sama_0195 0.61 +0.5 223,620 + Sama_0195 0.61 -1.1 223,620 + Sama_0195 0.61 +1.5 223,620 + Sama_0195 0.61 +0.7 223,620 + Sama_0195 0.61 +0.7 223,620 + Sama_0195 0.61 +0.4 223,621 - Sama_0195 0.62 +1.4 223,621 - Sama_0195 0.62 +0.5 223,621 - Sama_0195 0.62 +0.3 223,621 - Sama_0195 0.62 -1.0 223,621 - Sama_0195 0.62 -0.3 223,621 - Sama_0195 0.62 +0.1 223,621 - Sama_0195 0.62 +1.5 223,621 - Sama_0195 0.62 +1.1 223,672 + Sama_0195 0.73 +1.1 223,672 + Sama_0195 0.73 +0.7 223,673 - Sama_0195 0.73 +1.9 223,673 - Sama_0195 0.73 +0.9 223,673 - Sama_0195 0.73 +0.7 223,676 + Sama_0195 0.74 +0.1 223,676 + Sama_0195 0.74 +0.7 223,676 + Sama_0195 0.74 +0.8 223,676 + Sama_0195 0.74 +0.5 223,677 - Sama_0195 0.74 +0.9 223,677 - Sama_0195 0.74 +1.6 223,694 + Sama_0195 0.78 +0.4 223,694 + Sama_0195 0.78 +0.0 223,694 + Sama_0195 0.78 +0.1 223,694 + Sama_0195 0.78 +0.3 223,695 - Sama_0195 0.78 +0.7 223,695 - Sama_0195 0.78 +1.2 223,695 - Sama_0195 0.78 -2.4 223,699 + Sama_0195 0.79 +0.7 223,700 - Sama_0195 0.79 +1.0 223,710 + Sama_0195 0.81 +1.9 223,710 + Sama_0195 0.81 -0.4 223,710 + Sama_0195 0.81 -0.9 223,710 + Sama_0195 0.81 +0.8 223,727 + Sama_0195 0.85 +2.2 223,727 + Sama_0195 0.85 +0.1 223,728 - Sama_0195 0.85 -0.0 223,728 - Sama_0195 0.85 +1.2 223,777 + -0.2 223,778 - +0.4 223,820 - -1.6 223,831 - -1.8 223,842 - -2.1 223,851 + +1.4 223,851 + +1.2 223,852 - -1.7 223,853 + +0.6 223,853 + +1.7 223,853 + +1.0 223,853 + +1.3 223,854 - -1.9 223,854 - -0.9 223,854 - -1.4 223,855 + -1.2 223,855 + +1.0 223,855 + +0.7 223,855 + +0.5 223,855 + -1.9 223,855 + -0.9 223,855 + -0.5 223,855 + +1.0 223,856 - -1.5 223,856 - -1.5 223,856 - -2.4 223,856 - -0.9 223,856 - -1.1 223,856 - -1.6 223,856 - -2.4 223,856 - -2.9 223,856 - -1.2 223,856 - -1.4 223,856 - +0.2 223,859 - -4.3 223,876 + +1.2 223,877 - -1.8 223,934 - -1.3 224,005 - -1.4 224,061 + -0.1 224,062 - -1.4 224,062 - -0.8 224,063 + -0.2 224,063 + +0.7 224,063 + -0.9 224,063 + -0.6 224,064 - +0.4 224,064 - -3.5
Or see this region's nucleotide sequence