Experiment: Fusidic 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0977 and Psest_0978 are separated by 96 nucleotides Psest_0978 and Psest_0979 are separated by 127 nucleotides Psest_0979 and Psest_0980 are separated by 47 nucleotides Psest_0980 and Psest_0981 are separated by 28 nucleotides
Psest_0977: Psest_0977 - tRNA_Leu_GAG, at 1,060,289 to 1,060,374
_0977
Psest_0978: Psest_0978 - tRNA_Met_CAT, at 1,060,471 to 1,060,547
_0978
Psest_0979: Psest_0979 - Uncharacterized protein conserved in bacteria, at 1,060,675 to 1,061,133
_0979
Psest_0980: Psest_0980 - transcription termination factor NusA/transcription termination factor NusA, C-terminal duplication, at 1,061,181 to 1,062,662
_0980
Psest_0981: Psest_0981 - translation initiation factor IF-2, at 1,062,691 to 1,065,192
_0981
Position (kb)
1061
1062
1063 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1060.391 kb on + strand at 1060.391 kb on + strand at 1060.391 kb on + strand at 1060.422 kb on + strand at 1060.566 kb on + strand at 1060.579 kb on + strand at 1060.579 kb on + strand at 1060.652 kb on + strand at 1060.652 kb on + strand at 1060.652 kb on + strand at 1060.652 kb on + strand at 1060.652 kb on + strand at 1060.652 kb on + strand at 1060.652 kb on + strand at 1062.660 kb on + strand at 1062.660 kb on + strand at 1062.664 kb on + strand at 1062.664 kb on + strand at 1062.664 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.016 mg/ml remove 1,060,391 + +0.2 1,060,391 + -0.4 1,060,391 + -0.5 1,060,422 + +0.8 1,060,566 + -0.6 1,060,579 + -0.2 1,060,579 + -0.2 1,060,652 + -0.4 1,060,652 + +1.1 1,060,652 + -1.4 1,060,652 + -0.4 1,060,652 + +0.3 1,060,652 + -0.3 1,060,652 + -1.7 1,062,660 + -1.2 1,062,660 + +0.4 1,062,664 + -1.6 1,062,664 + -3.2 1,062,664 + -0.9
Or see this region's nucleotide sequence