Experiment: Fusidic 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0059 and Psest_0060 are separated by 2 nucleotides Psest_0060 and Psest_0061 are separated by 118 nucleotides Psest_0061 and Psest_0062 are separated by 175 nucleotides Psest_0062 and Psest_0063 overlap by 4 nucleotides
Psest_0059: Psest_0059 - hypothetical protein, at 60,782 to 61,153
_0059
Psest_0060: Psest_0060 - Predicted Fe-S protein, at 61,156 to 61,338
_0060
Psest_0061: Psest_0061 - hypothetical protein, at 61,457 to 61,786
_0061
Psest_0062: Psest_0062 - tryptophan synthase, alpha subunit, at 61,962 to 62,771
_0062
Psest_0063: Psest_0063 - tryptophan synthase, beta subunit, at 62,768 to 63,979
_0063
Position (kb) 61
62
63 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 61.215 kb on + strand, within Psest_0060 at 61.216 kb on - strand, within Psest_0060 at 61.290 kb on - strand, within Psest_0060 at 61.416 kb on + strand at 61.417 kb on - strand at 61.530 kb on + strand, within Psest_0061 at 61.530 kb on + strand, within Psest_0061 at 61.530 kb on + strand, within Psest_0061 at 61.531 kb on - strand, within Psest_0061 at 61.641 kb on + strand, within Psest_0061 at 61.653 kb on + strand, within Psest_0061 at 61.896 kb on + strand at 61.897 kb on - strand at 61.900 kb on + strand at 61.900 kb on + strand at 61.900 kb on + strand at 61.901 kb on - strand at 61.901 kb on - strand at 61.901 kb on - strand at 61.901 kb on - strand at 61.901 kb on - strand at 61.980 kb on + strand at 61.980 kb on + strand at 61.981 kb on - strand at 62.245 kb on + strand, within Psest_0062 at 62.245 kb on + strand, within Psest_0062 at 62.245 kb on + strand, within Psest_0062 at 62.245 kb on + strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.246 kb on - strand, within Psest_0062 at 62.248 kb on + strand, within Psest_0062 at 62.249 kb on - strand, within Psest_0062 at 62.249 kb on - strand, within Psest_0062 at 62.249 kb on - strand, within Psest_0062 at 62.464 kb on + strand, within Psest_0062 at 62.464 kb on + strand, within Psest_0062 at 62.464 kb on + strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.465 kb on - strand, within Psest_0062 at 62.684 kb on - strand, within Psest_0062 at 62.992 kb on - strand, within Psest_0063 at 62.992 kb on - strand, within Psest_0063 at 63.405 kb on + strand, within Psest_0063 at 63.405 kb on + strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.564 kb on + strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.742 kb on - strand, within Psest_0063 at 63.742 kb on - strand, within Psest_0063 at 63.742 kb on - strand, within Psest_0063 at 63.742 kb on - strand, within Psest_0063
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.016 mg/ml remove 61,215 + Psest_0060 0.32 -0.2 61,216 - Psest_0060 0.33 -0.2 61,290 - Psest_0060 0.73 -0.6 61,416 + +0.4 61,417 - -0.8 61,530 + Psest_0061 0.22 +2.6 61,530 + Psest_0061 0.22 +2.8 61,530 + Psest_0061 0.22 +0.6 61,531 - Psest_0061 0.22 -1.4 61,641 + Psest_0061 0.56 +1.6 61,653 + Psest_0061 0.59 +1.9 61,896 + +3.6 61,897 - -1.2 61,900 + +0.0 61,900 + +1.0 61,900 + +3.4 61,901 - -0.3 61,901 - -2.6 61,901 - -0.8 61,901 - +0.4 61,901 - +0.3 61,980 + -0.8 61,980 + +1.0 61,981 - +1.2 62,245 + Psest_0062 0.35 -2.9 62,245 + Psest_0062 0.35 -3.5 62,245 + Psest_0062 0.35 -2.0 62,245 + Psest_0062 0.35 -1.9 62,246 - Psest_0062 0.35 -0.3 62,246 - Psest_0062 0.35 -1.5 62,246 - Psest_0062 0.35 -1.6 62,246 - Psest_0062 0.35 -3.0 62,246 - Psest_0062 0.35 -0.7 62,248 + Psest_0062 0.35 -2.5 62,249 - Psest_0062 0.35 +0.4 62,249 - Psest_0062 0.35 -0.6 62,249 - Psest_0062 0.35 -1.1 62,464 + Psest_0062 0.62 -3.7 62,464 + Psest_0062 0.62 -2.8 62,464 + Psest_0062 0.62 -3.5 62,465 - Psest_0062 0.62 -2.0 62,465 - Psest_0062 0.62 -1.7 62,465 - Psest_0062 0.62 -1.9 62,465 - Psest_0062 0.62 -2.9 62,465 - Psest_0062 0.62 -1.0 62,465 - Psest_0062 0.62 -1.6 62,465 - Psest_0062 0.62 -1.3 62,465 - Psest_0062 0.62 -2.0 62,465 - Psest_0062 0.62 -4.2 62,465 - Psest_0062 0.62 -2.4 62,465 - Psest_0062 0.62 -1.9 62,465 - Psest_0062 0.62 -2.8 62,684 - Psest_0062 0.89 -3.8 62,992 - Psest_0063 0.18 -2.4 62,992 - Psest_0063 0.18 -3.1 63,405 + Psest_0063 0.53 +1.0 63,405 + Psest_0063 0.53 -0.3 63,406 - Psest_0063 0.53 -2.9 63,406 - Psest_0063 0.53 -2.8 63,406 - Psest_0063 0.53 -2.7 63,406 - Psest_0063 0.53 -0.7 63,406 - Psest_0063 0.53 -2.0 63,406 - Psest_0063 0.53 -1.3 63,406 - Psest_0063 0.53 +0.4 63,406 - Psest_0063 0.53 -2.6 63,564 + Psest_0063 0.66 -2.4 63,565 - Psest_0063 0.66 -2.1 63,565 - Psest_0063 0.66 -2.4 63,565 - Psest_0063 0.66 -0.6 63,565 - Psest_0063 0.66 -0.7 63,565 - Psest_0063 0.66 -2.9 63,741 + Psest_0063 0.80 -3.3 63,741 + Psest_0063 0.80 -1.0 63,741 + Psest_0063 0.80 -1.3 63,741 + Psest_0063 0.80 -2.0 63,741 + Psest_0063 0.80 -0.3 63,742 - Psest_0063 0.80 -2.9 63,742 - Psest_0063 0.80 -3.0 63,742 - Psest_0063 0.80 -1.4 63,742 - Psest_0063 0.80 -0.8
Or see this region's nucleotide sequence