Experiment: Fusidic 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3547 and Psest_3548 are separated by 12 nucleotides Psest_3548 and Psest_3549 overlap by 4 nucleotides Psest_3549 and Psest_3550 are separated by 59 nucleotides
Psest_3547: Psest_3547 - thiamine-monophosphate kinase, at 3,726,771 to 3,727,733
_3547
Psest_3548: Psest_3548 - transcription antitermination factor NusB, at 3,727,746 to 3,728,231
_3548
Psest_3549: Psest_3549 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,728,228 to 3,728,704
_3549
Psest_3550: Psest_3550 - 3,4-dihydroxy-2-butanone 4-phosphate synthase, at 3,728,764 to 3,729,843
_3550
Position (kb)
3727
3728
3729 Strain fitness (log2 ratio)
-1
0
1 at 3728.781 kb on - strand at 3728.781 kb on - strand at 3728.786 kb on - strand at 3728.786 kb on - strand at 3728.786 kb on - strand at 3728.786 kb on - strand at 3729.044 kb on - strand, within Psest_3550 at 3729.172 kb on - strand, within Psest_3550 at 3729.172 kb on - strand, within Psest_3550 at 3729.172 kb on - strand, within Psest_3550
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.016 mg/ml remove 3,728,781 - -0.9 3,728,781 - +0.7 3,728,786 - +0.7 3,728,786 - -1.3 3,728,786 - +0.8 3,728,786 - +1.6 3,729,044 - Psest_3550 0.26 +1.3 3,729,172 - Psest_3550 0.38 -1.4 3,729,172 - Psest_3550 0.38 +0.6 3,729,172 - Psest_3550 0.38 -0.3
Or see this region's nucleotide sequence