Experiment: Fusidic 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0258 and Psest_0259 overlap by 4 nucleotides Psest_0259 and Psest_0260 overlap by 4 nucleotides Psest_0260 and Psest_0261 are separated by 112 nucleotides
Psest_0258: Psest_0258 - signal recognition particle-docking protein FtsY, at 272,579 to 273,859
_0258
Psest_0259: Psest_0259 - cell division ATP-binding protein FtsE, at 273,856 to 274,527
_0259
Psest_0260: Psest_0260 - Cell division protein, at 274,524 to 275,543
_0260
Psest_0261: Psest_0261 - alternative sigma factor RpoH, at 275,656 to 276,510
_0261
Position (kb) 274
275
276 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 274.314 kb on - strand, within Psest_0259 at 274.347 kb on + strand, within Psest_0259 at 275.283 kb on + strand, within Psest_0260 at 275.284 kb on - strand, within Psest_0260 at 275.304 kb on + strand, within Psest_0260 at 275.305 kb on - strand, within Psest_0260 at 275.491 kb on + strand at 275.491 kb on + strand at 275.492 kb on - strand at 275.622 kb on + strand at 275.622 kb on + strand at 275.622 kb on + strand at 276.524 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.016 mg/ml remove 274,314 - Psest_0259 0.68 -3.5 274,347 + Psest_0259 0.73 -3.4 275,283 + Psest_0260 0.74 -2.5 275,284 - Psest_0260 0.75 -2.2 275,304 + Psest_0260 0.76 -2.2 275,305 - Psest_0260 0.77 +2.2 275,491 + -2.8 275,491 + -0.9 275,492 - -2.3 275,622 + +0.6 275,622 + +1.6 275,622 + +0.2 276,524 - -0.4
Or see this region's nucleotide sequence