Experiment: Fusidic 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1678 and Psest_1679 are separated by 390 nucleotides Psest_1679 and Psest_1680 are separated by 559 nucleotides
Psest_1678: Psest_1678 - UDP-N-acetylenolpyruvoylglucosamine reductase, at 1,785,074 to 1,786,093
_1678
Psest_1679: Psest_1679 - ribonuclease, Rne/Rng family, at 1,786,484 to 1,789,666
_1679
Psest_1680: Psest_1680 - pseudouridine synthase, RluA family, at 1,790,226 to 1,791,179
_1680
Position (kb)
1786
1787
1788
1789
1790 Strain fitness (log2 ratio)
-2
-1
0
1 at 1786.147 kb on - strand at 1786.147 kb on - strand at 1786.236 kb on + strand at 1786.236 kb on + strand at 1786.237 kb on - strand at 1786.336 kb on + strand at 1786.337 kb on - strand at 1786.337 kb on - strand at 1786.337 kb on - strand at 1786.338 kb on + strand at 1786.353 kb on - strand at 1786.383 kb on + strand at 1786.384 kb on - strand at 1786.410 kb on + strand at 1786.410 kb on + strand at 1786.410 kb on + strand at 1786.411 kb on - strand at 1786.595 kb on + strand at 1786.596 kb on - strand at 1786.707 kb on - strand at 1787.061 kb on - strand, within Psest_1679 at 1787.428 kb on + strand, within Psest_1679 at 1787.428 kb on + strand, within Psest_1679 at 1787.429 kb on - strand, within Psest_1679 at 1790.057 kb on - strand at 1790.188 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.016 mg/ml remove 1,786,147 - -1.3 1,786,147 - +0.2 1,786,236 + +0.2 1,786,236 + -0.4 1,786,237 - -2.0 1,786,336 + -1.2 1,786,337 - -0.4 1,786,337 - -0.9 1,786,337 - +0.1 1,786,338 + +0.4 1,786,353 - +0.5 1,786,383 + -1.3 1,786,384 - -1.3 1,786,410 + +0.2 1,786,410 + -0.0 1,786,410 + +1.0 1,786,411 - +1.0 1,786,595 + -2.3 1,786,596 - +0.3 1,786,707 - +0.6 1,787,061 - Psest_1679 0.18 +0.7 1,787,428 + Psest_1679 0.30 -2.0 1,787,428 + Psest_1679 0.30 -0.8 1,787,429 - Psest_1679 0.30 -1.4 1,790,057 - +1.4 1,790,188 - +0.9
Or see this region's nucleotide sequence