Experiment: Fusidic 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1494 and Psest_1495 are separated by 155 nucleotides Psest_1495 and Psest_1496 are separated by 73 nucleotides Psest_1496 and Psest_1497 are separated by 3 nucleotides
Psest_1494: Psest_1494 - DNA-binding regulatory protein, YebC/PmpR family, at 1,596,654 to 1,597,403
_1494
Psest_1495: Psest_1495 - crossover junction endodeoxyribonuclease RuvC, at 1,597,559 to 1,598,083
_1495
Psest_1496: Psest_1496 - Holliday junction DNA helicase, RuvA subunit, at 1,598,157 to 1,598,762
_1496
Psest_1497: Psest_1497 - Holliday junction DNA helicase, RuvB subunit, at 1,598,766 to 1,599,818
_1497
Position (kb)
1597
1598
1599 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1597.435 kb on + strand at 1597.435 kb on + strand at 1597.435 kb on + strand at 1597.436 kb on - strand at 1597.520 kb on + strand at 1597.521 kb on - strand at 1597.537 kb on - strand at 1597.540 kb on + strand at 1597.540 kb on + strand at 1598.113 kb on - strand at 1598.332 kb on + strand, within Psest_1496 at 1598.333 kb on - strand, within Psest_1496 at 1598.333 kb on - strand, within Psest_1496 at 1598.760 kb on + strand at 1598.760 kb on + strand at 1598.760 kb on + strand at 1598.760 kb on + strand at 1598.761 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.016 mg/ml remove 1,597,435 + +0.3 1,597,435 + -0.1 1,597,435 + -1.7 1,597,436 - -2.3 1,597,520 + +0.4 1,597,521 - +1.6 1,597,537 - -1.2 1,597,540 + -0.8 1,597,540 + -3.0 1,598,113 - -2.7 1,598,332 + Psest_1496 0.29 -0.9 1,598,333 - Psest_1496 0.29 -3.7 1,598,333 - Psest_1496 0.29 -2.8 1,598,760 + -0.0 1,598,760 + -0.5 1,598,760 + -3.6 1,598,760 + -2.2 1,598,761 - -1.2
Or see this region's nucleotide sequence