Experiment: Paraquat dichloride 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secD and yajC are separated by 18 nucleotides yajC and tgt are separated by 26 nucleotides tgt and queA are separated by 152 nucleotides
Sama_2191: secD - preprotein translocase subunit SecD (RefSeq), at 2,649,111 to 2,650,961
secD
Sama_2192: yajC - preprotein translocase subunit YajC (RefSeq), at 2,650,980 to 2,651,315
yajC
Sama_2193: tgt - queuine tRNA-ribosyltransferase (RefSeq), at 2,651,342 to 2,652,466
tgt
Sama_2194: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (RefSeq), at 2,652,619 to 2,653,656
queA
Position (kb)
2651
2652
2653 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2650.476 kb on - strand, within secD at 2650.895 kb on - strand at 2651.344 kb on - strand at 2651.344 kb on - strand at 2651.352 kb on - strand at 2651.383 kb on - strand at 2651.383 kb on - strand at 2651.383 kb on - strand at 2651.383 kb on - strand at 2651.383 kb on - strand at 2651.406 kb on - strand at 2651.458 kb on - strand, within tgt at 2651.477 kb on - strand, within tgt at 2651.551 kb on + strand, within tgt at 2651.554 kb on - strand, within tgt at 2651.567 kb on - strand, within tgt at 2651.670 kb on - strand, within tgt at 2651.742 kb on + strand, within tgt at 2651.839 kb on - strand, within tgt at 2651.895 kb on + strand, within tgt at 2651.896 kb on - strand, within tgt at 2651.896 kb on - strand, within tgt at 2652.053 kb on + strand, within tgt at 2652.183 kb on - strand, within tgt at 2652.364 kb on - strand at 2652.368 kb on - strand at 2652.446 kb on - strand at 2652.448 kb on + strand at 2652.634 kb on - strand at 2652.696 kb on - strand at 2652.706 kb on + strand at 2652.707 kb on - strand at 2652.707 kb on - strand at 2652.791 kb on - strand, within queA at 2652.817 kb on - strand, within queA at 2652.817 kb on - strand, within queA at 2652.822 kb on + strand, within queA at 2652.823 kb on - strand, within queA at 2652.968 kb on - strand, within queA at 2653.012 kb on + strand, within queA at 2653.126 kb on - strand, within queA at 2653.157 kb on + strand, within queA at 2653.157 kb on + strand, within queA at 2653.157 kb on + strand, within queA at 2653.158 kb on - strand, within queA at 2653.158 kb on - strand, within queA at 2653.206 kb on + strand, within queA at 2653.207 kb on - strand, within queA at 2653.208 kb on + strand, within queA at 2653.209 kb on - strand, within queA at 2653.224 kb on + strand, within queA at 2653.260 kb on + strand, within queA at 2653.260 kb on + strand, within queA at 2653.261 kb on - strand, within queA at 2653.261 kb on - strand, within queA at 2653.404 kb on + strand, within queA at 2653.405 kb on - strand, within queA at 2653.405 kb on - strand, within queA at 2653.405 kb on - strand, within queA at 2653.405 kb on - strand, within queA at 2653.406 kb on - strand, within queA
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.03125 mg/ml remove 2,650,476 - secD Sama_2191 0.74 +1.2 2,650,895 - -2.3 2,651,344 - -2.3 2,651,344 - -0.8 2,651,352 - -1.1 2,651,383 - -1.8 2,651,383 - -1.8 2,651,383 - -1.9 2,651,383 - -3.0 2,651,383 - -2.3 2,651,406 - -2.7 2,651,458 - tgt Sama_2193 0.10 -0.3 2,651,477 - tgt Sama_2193 0.12 -0.8 2,651,551 + tgt Sama_2193 0.19 -1.3 2,651,554 - tgt Sama_2193 0.19 -1.8 2,651,567 - tgt Sama_2193 0.20 -0.6 2,651,670 - tgt Sama_2193 0.29 -0.1 2,651,742 + tgt Sama_2193 0.36 -1.8 2,651,839 - tgt Sama_2193 0.44 -1.3 2,651,895 + tgt Sama_2193 0.49 -1.6 2,651,896 - tgt Sama_2193 0.49 +0.5 2,651,896 - tgt Sama_2193 0.49 -1.8 2,652,053 + tgt Sama_2193 0.63 -1.6 2,652,183 - tgt Sama_2193 0.75 -0.9 2,652,364 - -1.1 2,652,368 - -0.8 2,652,446 - -0.3 2,652,448 + -0.3 2,652,634 - +0.5 2,652,696 - -0.3 2,652,706 + +0.2 2,652,707 - -1.1 2,652,707 - -0.0 2,652,791 - queA Sama_2194 0.17 -2.3 2,652,817 - queA Sama_2194 0.19 -0.8 2,652,817 - queA Sama_2194 0.19 -0.1 2,652,822 + queA Sama_2194 0.20 -1.2 2,652,823 - queA Sama_2194 0.20 +0.9 2,652,968 - queA Sama_2194 0.34 -1.7 2,653,012 + queA Sama_2194 0.38 -1.8 2,653,126 - queA Sama_2194 0.49 -0.4 2,653,157 + queA Sama_2194 0.52 -1.2 2,653,157 + queA Sama_2194 0.52 -1.6 2,653,157 + queA Sama_2194 0.52 +2.2 2,653,158 - queA Sama_2194 0.52 -1.3 2,653,158 - queA Sama_2194 0.52 +0.0 2,653,206 + queA Sama_2194 0.57 -0.3 2,653,207 - queA Sama_2194 0.57 -1.2 2,653,208 + queA Sama_2194 0.57 +0.9 2,653,209 - queA Sama_2194 0.57 -1.0 2,653,224 + queA Sama_2194 0.58 -0.8 2,653,260 + queA Sama_2194 0.62 -1.9 2,653,260 + queA Sama_2194 0.62 +0.7 2,653,261 - queA Sama_2194 0.62 -1.6 2,653,261 - queA Sama_2194 0.62 -1.8 2,653,404 + queA Sama_2194 0.76 -1.1 2,653,405 - queA Sama_2194 0.76 -1.3 2,653,405 - queA Sama_2194 0.76 -0.1 2,653,405 - queA Sama_2194 0.76 +1.1 2,653,405 - queA Sama_2194 0.76 -0.2 2,653,406 - queA Sama_2194 0.76 -0.6
Or see this region's nucleotide sequence