Experiment: Paraquat dichloride 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_1949 and Sama_1950 are separated by 56 nucleotides Sama_1950 and ihfA are separated by 81 nucleotides ihfA and pheT are separated by 3 nucleotides
Sama_1949: Sama_1949 - diguanylate cyclase, putative (RefSeq), at 2,376,276 to 2,377,259
_1949
Sama_1950: Sama_1950 - lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (RefSeq), at 2,377,316 to 2,378,242
_1950
Sama_1951: ihfA - integration host factor subunit alpha (RefSeq), at 2,378,324 to 2,378,620
ihfA
Sama_1952: pheT - phenylalanyl-tRNA synthetase subunit beta (RefSeq), at 2,378,624 to 2,381,011
pheT
Position (kb)
2377
2378
2379 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2376.326 kb on + strand at 2376.326 kb on + strand at 2376.326 kb on + strand at 2376.326 kb on + strand at 2376.326 kb on + strand at 2376.326 kb on + strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.327 kb on - strand at 2376.348 kb on + strand at 2376.392 kb on + strand, within Sama_1949 at 2376.435 kb on - strand, within Sama_1949 at 2376.468 kb on + strand, within Sama_1949 at 2376.534 kb on + strand, within Sama_1949 at 2376.537 kb on - strand, within Sama_1949 at 2376.572 kb on + strand, within Sama_1949 at 2376.830 kb on - strand, within Sama_1949 at 2376.866 kb on + strand, within Sama_1949 at 2376.867 kb on - strand, within Sama_1949 at 2376.897 kb on - strand, within Sama_1949 at 2376.932 kb on + strand, within Sama_1949 at 2377.113 kb on + strand, within Sama_1949 at 2377.195 kb on - strand at 2377.195 kb on - strand at 2377.277 kb on - strand at 2377.290 kb on + strand at 2377.290 kb on + strand at 2377.291 kb on - strand at 2377.291 kb on - strand at 2377.291 kb on - strand at 2377.291 kb on - strand at 2377.291 kb on - strand at 2377.291 kb on - strand at 2377.292 kb on + strand at 2377.324 kb on - strand at 2377.376 kb on - strand at 2377.378 kb on - strand at 2377.611 kb on - strand, within Sama_1950 at 2377.710 kb on + strand, within Sama_1950 at 2377.768 kb on + strand, within Sama_1950 at 2377.832 kb on + strand, within Sama_1950 at 2377.842 kb on - strand, within Sama_1950 at 2378.051 kb on - strand, within Sama_1950 at 2378.254 kb on - strand at 2378.265 kb on - strand at 2378.541 kb on - strand, within ihfA at 2378.541 kb on - strand, within ihfA at 2378.541 kb on - strand, within ihfA at 2378.626 kb on - strand at 2378.626 kb on - strand at 2378.626 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.03125 mg/ml remove 2,376,326 + -2.0 2,376,326 + +1.5 2,376,326 + +0.8 2,376,326 + -0.9 2,376,326 + -0.4 2,376,326 + +0.1 2,376,327 - +0.8 2,376,327 - -0.4 2,376,327 - -0.7 2,376,327 - -0.7 2,376,327 - +0.7 2,376,327 - +1.4 2,376,327 - -0.1 2,376,327 - -1.1 2,376,327 - -0.8 2,376,327 - -2.4 2,376,327 - +0.5 2,376,348 + -0.6 2,376,392 + Sama_1949 0.12 -2.6 2,376,435 - Sama_1949 0.16 -0.8 2,376,468 + Sama_1949 0.20 -0.5 2,376,534 + Sama_1949 0.26 -1.5 2,376,537 - Sama_1949 0.27 -0.7 2,376,572 + Sama_1949 0.30 -1.1 2,376,830 - Sama_1949 0.56 -0.4 2,376,866 + Sama_1949 0.60 -0.0 2,376,867 - Sama_1949 0.60 +2.0 2,376,897 - Sama_1949 0.63 +0.1 2,376,932 + Sama_1949 0.67 -0.6 2,377,113 + Sama_1949 0.85 +0.2 2,377,195 - -0.4 2,377,195 - +0.0 2,377,277 - +0.1 2,377,290 + -1.8 2,377,290 + +0.1 2,377,291 - -0.4 2,377,291 - -2.0 2,377,291 - +0.2 2,377,291 - -0.1 2,377,291 - -0.1 2,377,291 - +1.3 2,377,292 + -0.7 2,377,324 - -3.1 2,377,376 - -0.4 2,377,378 - -1.8 2,377,611 - Sama_1950 0.32 -2.0 2,377,710 + Sama_1950 0.43 -1.6 2,377,768 + Sama_1950 0.49 -2.0 2,377,832 + Sama_1950 0.56 -1.0 2,377,842 - Sama_1950 0.57 -1.4 2,378,051 - Sama_1950 0.79 -1.4 2,378,254 - -0.8 2,378,265 - +0.0 2,378,541 - ihfA Sama_1951 0.73 +0.8 2,378,541 - ihfA Sama_1951 0.73 +1.0 2,378,541 - ihfA Sama_1951 0.73 +0.0 2,378,626 - -0.4 2,378,626 - -1.4 2,378,626 - +1.4
Or see this region's nucleotide sequence