Experiment: Paraquat dichloride 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_1853 and Sama_1854 are separated by 5 nucleotides Sama_1854 and thiH are separated by 114 nucleotides thiH and thiG overlap by 4 nucleotides thiG and Sama_1857 are separated by 1 nucleotides
Sama_1853: Sama_1853 - hypothetical protein (RefSeq), at 2,257,149 to 2,258,933
_1853
Sama_1854: Sama_1854 - methyl-accepting chemotaxis protein (RefSeq), at 2,258,939 to 2,259,190
_1854
Sama_1855: thiH - thiamine biosynthesis protein ThiH (RefSeq), at 2,259,305 to 2,260,420
thiH
Sama_1856: thiG - thiazole synthase (RefSeq), at 2,260,417 to 2,261,232
thiG
Sama_1857: Sama_1857 - thiamine biosynthesis protein ThiS (RefSeq), at 2,261,234 to 2,261,437
_1857
Position (kb)
2259
2260
2261 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2258.309 kb on + strand, within Sama_1853 at 2258.310 kb on - strand, within Sama_1853 at 2258.310 kb on - strand, within Sama_1853 at 2258.346 kb on - strand, within Sama_1853 at 2258.346 kb on - strand, within Sama_1853 at 2258.456 kb on + strand, within Sama_1853 at 2258.634 kb on + strand, within Sama_1853 at 2258.634 kb on + strand, within Sama_1853 at 2258.635 kb on - strand, within Sama_1853 at 2258.638 kb on - strand, within Sama_1853 at 2258.667 kb on + strand, within Sama_1853 at 2258.668 kb on - strand, within Sama_1853 at 2258.811 kb on + strand at 2258.812 kb on - strand at 2258.940 kb on + strand at 2258.941 kb on - strand at 2258.954 kb on + strand at 2258.954 kb on + strand at 2258.954 kb on + strand at 2258.954 kb on + strand at 2258.955 kb on - strand at 2258.955 kb on - strand at 2258.955 kb on - strand at 2258.955 kb on - strand at 2258.955 kb on - strand at 2259.053 kb on - strand, within Sama_1854 at 2259.206 kb on - strand at 2259.206 kb on - strand at 2259.283 kb on - strand at 2259.307 kb on - strand at 2259.340 kb on + strand at 2259.340 kb on + strand at 2259.340 kb on + strand at 2259.341 kb on - strand at 2259.432 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.568 kb on + strand, within thiH at 2259.568 kb on + strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.587 kb on - strand, within thiH at 2259.649 kb on + strand, within thiH at 2259.649 kb on + strand, within thiH at 2259.684 kb on + strand, within thiH at 2259.793 kb on + strand, within thiH at 2259.809 kb on - strand, within thiH at 2259.847 kb on - strand, within thiH at 2259.847 kb on - strand, within thiH at 2259.847 kb on - strand, within thiH at 2259.847 kb on - strand, within thiH at 2259.862 kb on - strand, within thiH at 2259.955 kb on - strand, within thiH at 2259.988 kb on + strand, within thiH at 2260.051 kb on - strand, within thiH at 2260.085 kb on - strand, within thiH at 2260.115 kb on - strand, within thiH at 2260.143 kb on + strand, within thiH at 2260.176 kb on + strand, within thiH at 2260.176 kb on + strand, within thiH at 2260.176 kb on + strand, within thiH at 2260.177 kb on - strand, within thiH at 2260.177 kb on - strand, within thiH at 2260.188 kb on + strand, within thiH at 2260.188 kb on + strand, within thiH at 2260.188 kb on + strand, within thiH at 2260.189 kb on - strand, within thiH at 2260.189 kb on - strand, within thiH at 2260.192 kb on + strand, within thiH at 2260.192 kb on + strand, within thiH at 2260.192 kb on + strand, within thiH at 2260.193 kb on - strand, within thiH at 2260.217 kb on - strand, within thiH at 2260.257 kb on + strand, within thiH at 2260.353 kb on + strand at 2260.372 kb on + strand at 2260.372 kb on + strand at 2260.372 kb on + strand at 2260.408 kb on - strand at 2260.474 kb on - strand at 2260.621 kb on - strand, within thiG at 2260.623 kb on + strand, within thiG at 2260.768 kb on + strand, within thiG at 2260.847 kb on + strand, within thiG at 2260.847 kb on + strand, within thiG at 2260.847 kb on + strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.848 kb on - strand, within thiG at 2260.855 kb on + strand, within thiG at 2260.856 kb on - strand, within thiG at 2260.856 kb on - strand, within thiG at 2260.861 kb on - strand, within thiG at 2260.861 kb on - strand, within thiG at 2260.861 kb on - strand, within thiG at 2260.922 kb on + strand, within thiG at 2260.923 kb on - strand, within thiG at 2261.164 kb on - strand at 2261.281 kb on + strand, within Sama_1857 at 2261.290 kb on + strand, within Sama_1857 at 2261.291 kb on - strand, within Sama_1857 at 2261.291 kb on - strand, within Sama_1857 at 2261.291 kb on - strand, within Sama_1857 at 2261.291 kb on - strand, within Sama_1857 at 2261.302 kb on + strand, within Sama_1857 at 2261.303 kb on - strand, within Sama_1857 at 2261.315 kb on + strand, within Sama_1857 at 2261.315 kb on + strand, within Sama_1857 at 2261.316 kb on - strand, within Sama_1857 at 2261.316 kb on - strand, within Sama_1857 at 2261.316 kb on - strand, within Sama_1857 at 2261.369 kb on - strand, within Sama_1857
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.03125 mg/ml remove 2,258,309 + Sama_1853 0.65 -0.3 2,258,310 - Sama_1853 0.65 +0.9 2,258,310 - Sama_1853 0.65 +0.8 2,258,346 - Sama_1853 0.67 +1.2 2,258,346 - Sama_1853 0.67 -0.3 2,258,456 + Sama_1853 0.73 +0.8 2,258,634 + Sama_1853 0.83 -0.2 2,258,634 + Sama_1853 0.83 +1.3 2,258,635 - Sama_1853 0.83 -0.4 2,258,638 - Sama_1853 0.83 +0.4 2,258,667 + Sama_1853 0.85 +0.1 2,258,668 - Sama_1853 0.85 -0.3 2,258,811 + +0.2 2,258,812 - -1.6 2,258,940 + +1.0 2,258,941 - +1.0 2,258,954 + -0.6 2,258,954 + -0.4 2,258,954 + +0.5 2,258,954 + +0.1 2,258,955 - -1.8 2,258,955 - +1.0 2,258,955 - +0.1 2,258,955 - +0.5 2,258,955 - +0.0 2,259,053 - Sama_1854 0.45 -1.0 2,259,206 - +0.6 2,259,206 - -0.5 2,259,283 - +0.6 2,259,307 - -0.4 2,259,340 + -0.4 2,259,340 + -1.2 2,259,340 + +0.2 2,259,341 - +0.2 2,259,432 + thiH Sama_1855 0.11 -0.3 2,259,448 + thiH Sama_1855 0.13 -2.3 2,259,448 + thiH Sama_1855 0.13 +0.8 2,259,448 + thiH Sama_1855 0.13 -0.6 2,259,448 + thiH Sama_1855 0.13 -0.8 2,259,448 + thiH Sama_1855 0.13 -0.6 2,259,449 - thiH Sama_1855 0.13 -0.2 2,259,449 - thiH Sama_1855 0.13 -1.2 2,259,449 - thiH Sama_1855 0.13 -0.2 2,259,449 - thiH Sama_1855 0.13 +1.8 2,259,449 - thiH Sama_1855 0.13 -0.1 2,259,568 + thiH Sama_1855 0.24 +0.7 2,259,568 + thiH Sama_1855 0.24 +0.7 2,259,569 - thiH Sama_1855 0.24 +1.2 2,259,569 - thiH Sama_1855 0.24 -0.5 2,259,569 - thiH Sama_1855 0.24 -0.3 2,259,569 - thiH Sama_1855 0.24 +0.4 2,259,569 - thiH Sama_1855 0.24 +0.4 2,259,587 - thiH Sama_1855 0.25 -0.6 2,259,649 + thiH Sama_1855 0.31 -0.8 2,259,649 + thiH Sama_1855 0.31 -1.4 2,259,684 + thiH Sama_1855 0.34 +0.8 2,259,793 + thiH Sama_1855 0.44 +0.0 2,259,809 - thiH Sama_1855 0.45 +3.2 2,259,847 - thiH Sama_1855 0.49 +1.0 2,259,847 - thiH Sama_1855 0.49 +0.2 2,259,847 - thiH Sama_1855 0.49 -3.0 2,259,847 - thiH Sama_1855 0.49 +0.1 2,259,862 - thiH Sama_1855 0.50 +0.3 2,259,955 - thiH Sama_1855 0.58 +1.2 2,259,988 + thiH Sama_1855 0.61 +0.1 2,260,051 - thiH Sama_1855 0.67 -0.2 2,260,085 - thiH Sama_1855 0.70 +0.4 2,260,115 - thiH Sama_1855 0.73 -1.4 2,260,143 + thiH Sama_1855 0.75 +0.6 2,260,176 + thiH Sama_1855 0.78 +1.2 2,260,176 + thiH Sama_1855 0.78 -0.8 2,260,176 + thiH Sama_1855 0.78 +0.8 2,260,177 - thiH Sama_1855 0.78 +0.1 2,260,177 - thiH Sama_1855 0.78 +0.1 2,260,188 + thiH Sama_1855 0.79 -0.6 2,260,188 + thiH Sama_1855 0.79 -0.3 2,260,188 + thiH Sama_1855 0.79 +0.8 2,260,189 - thiH Sama_1855 0.79 -1.8 2,260,189 - thiH Sama_1855 0.79 -1.6 2,260,192 + thiH Sama_1855 0.79 +0.7 2,260,192 + thiH Sama_1855 0.79 +1.0 2,260,192 + thiH Sama_1855 0.79 -0.7 2,260,193 - thiH Sama_1855 0.80 +1.8 2,260,217 - thiH Sama_1855 0.82 +0.4 2,260,257 + thiH Sama_1855 0.85 -1.6 2,260,353 + -0.6 2,260,372 + +0.6 2,260,372 + -0.3 2,260,372 + +1.6 2,260,408 - +1.2 2,260,474 - -3.1 2,260,621 - thiG Sama_1856 0.25 -0.6 2,260,623 + thiG Sama_1856 0.25 -0.6 2,260,768 + thiG Sama_1856 0.43 +1.5 2,260,847 + thiG Sama_1856 0.53 +1.5 2,260,847 + thiG Sama_1856 0.53 -0.4 2,260,847 + thiG Sama_1856 0.53 -0.4 2,260,848 - thiG Sama_1856 0.53 +0.3 2,260,848 - thiG Sama_1856 0.53 +1.0 2,260,848 - thiG Sama_1856 0.53 -1.3 2,260,848 - thiG Sama_1856 0.53 -0.4 2,260,848 - thiG Sama_1856 0.53 -1.8 2,260,848 - thiG Sama_1856 0.53 +0.8 2,260,848 - thiG Sama_1856 0.53 -0.3 2,260,848 - thiG Sama_1856 0.53 +0.4 2,260,855 + thiG Sama_1856 0.54 +0.7 2,260,856 - thiG Sama_1856 0.54 -0.1 2,260,856 - thiG Sama_1856 0.54 +0.7 2,260,861 - thiG Sama_1856 0.54 +0.2 2,260,861 - thiG Sama_1856 0.54 -0.4 2,260,861 - thiG Sama_1856 0.54 +0.7 2,260,922 + thiG Sama_1856 0.62 -0.4 2,260,923 - thiG Sama_1856 0.62 +1.0 2,261,164 - +0.6 2,261,281 + Sama_1857 0.23 -1.6 2,261,290 + Sama_1857 0.27 +0.0 2,261,291 - Sama_1857 0.28 +0.0 2,261,291 - Sama_1857 0.28 +0.6 2,261,291 - Sama_1857 0.28 +1.1 2,261,291 - Sama_1857 0.28 -1.6 2,261,302 + Sama_1857 0.33 +1.2 2,261,303 - Sama_1857 0.34 -0.4 2,261,315 + Sama_1857 0.40 +0.7 2,261,315 + Sama_1857 0.40 -0.1 2,261,316 - Sama_1857 0.40 -0.4 2,261,316 - Sama_1857 0.40 -1.8 2,261,316 - Sama_1857 0.40 -1.3 2,261,369 - Sama_1857 0.66 +0.8
Or see this region's nucleotide sequence