Experiment: Paraquat dichloride 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_1222 and Sama_R0046 are separated by 237 nucleotides Sama_R0046 and Sama_R0047 are separated by 64 nucleotides Sama_R0047 and Sama_R0048 are separated by 12 nucleotides Sama_R0048 and tig are separated by 252 nucleotides tig and clpP are separated by 89 nucleotides clpP and clpX are separated by 85 nucleotides
Sama_1222: Sama_1222 - methylenetetrahydrofolate dehydrogenase (NADP(+)) (RefSeq), at 1,503,327 to 1,504,181
_1222
Sama_R0046: Sama_R0046 - tRNA-Pro (RefSeq), at 1,504,419 to 1,504,495
_R0046
Sama_R0047: Sama_R0047 - tRNA-Arg (RefSeq), at 1,504,560 to 1,504,636
_R0047
Sama_R0048: Sama_R0048 - tRNA-His (RefSeq), at 1,504,649 to 1,504,724
_R0048
Sama_1223: tig - trigger factor (RefSeq), at 1,504,977 to 1,506,281
tig
Sama_1224: clpP - ATP-dependent Clp protease proteolytic subunit (RefSeq), at 1,506,371 to 1,506,979
clpP
Sama_1225: clpX - ATP-dependent protease ATP-binding subunit ClpX (RefSeq), at 1,507,065 to 1,508,342
clpX
Position (kb)
1504
1505
1506
1507 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1504.303 kb on + strand at 1504.304 kb on - strand at 1504.312 kb on - strand at 1504.313 kb on + strand at 1504.314 kb on - strand at 1504.484 kb on + strand, within Sama_R0046 at 1504.499 kb on + strand at 1504.499 kb on + strand at 1504.501 kb on + strand at 1504.501 kb on + strand at 1504.501 kb on + strand at 1504.515 kb on + strand at 1504.554 kb on + strand at 1504.554 kb on + strand at 1504.739 kb on + strand at 1504.774 kb on + strand at 1504.815 kb on + strand at 1504.815 kb on + strand at 1504.919 kb on + strand at 1504.919 kb on + strand at 1504.919 kb on + strand at 1504.919 kb on + strand at 1504.920 kb on - strand at 1504.923 kb on - strand at 1504.942 kb on + strand at 1504.943 kb on - strand at 1504.954 kb on + strand at 1504.954 kb on + strand at 1504.955 kb on - strand at 1504.960 kb on + strand at 1504.960 kb on + strand at 1504.960 kb on + strand at 1504.961 kb on - strand at 1505.060 kb on + strand at 1505.118 kb on - strand, within tig at 1505.122 kb on + strand, within tig at 1505.122 kb on + strand, within tig at 1505.126 kb on + strand, within tig at 1505.126 kb on + strand, within tig at 1505.126 kb on + strand, within tig at 1505.148 kb on + strand, within tig at 1505.246 kb on + strand, within tig at 1505.246 kb on + strand, within tig at 1505.317 kb on + strand, within tig at 1505.317 kb on + strand, within tig at 1505.420 kb on + strand, within tig at 1505.420 kb on + strand, within tig at 1505.420 kb on + strand, within tig at 1505.420 kb on + strand, within tig at 1505.420 kb on + strand, within tig at 1505.552 kb on + strand, within tig at 1505.634 kb on + strand, within tig at 1505.634 kb on + strand, within tig at 1505.716 kb on + strand, within tig at 1505.716 kb on + strand, within tig at 1505.770 kb on + strand, within tig at 1505.911 kb on + strand, within tig at 1505.911 kb on + strand, within tig at 1505.911 kb on + strand, within tig at 1506.044 kb on + strand, within tig at 1506.092 kb on + strand, within tig at 1506.092 kb on + strand, within tig at 1506.132 kb on + strand, within tig at 1506.135 kb on + strand, within tig at 1506.135 kb on + strand, within tig at 1506.254 kb on + strand at 1506.254 kb on + strand at 1506.314 kb on + strand at 1506.314 kb on + strand at 1506.326 kb on + strand at 1506.326 kb on + strand at 1506.353 kb on + strand at 1506.449 kb on + strand, within clpP at 1506.449 kb on + strand, within clpP at 1506.450 kb on - strand, within clpP at 1506.450 kb on - strand, within clpP at 1506.458 kb on + strand, within clpP at 1506.458 kb on + strand, within clpP at 1506.458 kb on + strand, within clpP at 1506.458 kb on + strand, within clpP at 1506.458 kb on + strand, within clpP at 1506.458 kb on + strand, within clpP at 1506.459 kb on - strand, within clpP at 1506.459 kb on - strand, within clpP at 1506.578 kb on + strand, within clpP at 1506.578 kb on + strand, within clpP at 1506.578 kb on + strand, within clpP at 1506.579 kb on - strand, within clpP at 1506.584 kb on + strand, within clpP at 1506.585 kb on - strand, within clpP at 1506.585 kb on - strand, within clpP at 1506.629 kb on + strand, within clpP at 1506.634 kb on + strand, within clpP at 1506.637 kb on + strand, within clpP at 1506.637 kb on + strand, within clpP at 1506.637 kb on + strand, within clpP at 1506.637 kb on + strand, within clpP at 1506.637 kb on + strand, within clpP at 1506.638 kb on - strand, within clpP at 1506.691 kb on + strand, within clpP at 1506.691 kb on + strand, within clpP at 1506.859 kb on - strand, within clpP at 1506.944 kb on + strand at 1507.023 kb on + strand at 1507.104 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.03125 mg/ml remove 1,504,303 + +0.3 1,504,304 - +0.3 1,504,312 - +0.8 1,504,313 + -0.1 1,504,314 - +1.3 1,504,484 + Sama_R0046 0.84 +1.9 1,504,499 + -2.9 1,504,499 + +2.2 1,504,501 + +0.1 1,504,501 + -0.9 1,504,501 + +0.9 1,504,515 + -0.5 1,504,554 + +0.3 1,504,554 + +0.0 1,504,739 + +1.2 1,504,774 + -0.0 1,504,815 + -1.5 1,504,815 + -1.0 1,504,919 + -1.8 1,504,919 + -1.5 1,504,919 + -2.1 1,504,919 + -1.0 1,504,920 - -1.7 1,504,923 - -1.5 1,504,942 + -2.5 1,504,943 - -1.2 1,504,954 + -3.6 1,504,954 + -1.5 1,504,955 - +0.1 1,504,960 + -0.5 1,504,960 + +0.0 1,504,960 + -1.2 1,504,961 - +0.9 1,505,060 + -1.0 1,505,118 - tig Sama_1223 0.11 -2.2 1,505,122 + tig Sama_1223 0.11 -1.7 1,505,122 + tig Sama_1223 0.11 -2.4 1,505,126 + tig Sama_1223 0.11 +0.3 1,505,126 + tig Sama_1223 0.11 -1.7 1,505,126 + tig Sama_1223 0.11 -0.5 1,505,148 + tig Sama_1223 0.13 -2.1 1,505,246 + tig Sama_1223 0.21 -1.6 1,505,246 + tig Sama_1223 0.21 -1.2 1,505,317 + tig Sama_1223 0.26 -1.5 1,505,317 + tig Sama_1223 0.26 -1.8 1,505,420 + tig Sama_1223 0.34 -1.4 1,505,420 + tig Sama_1223 0.34 -1.7 1,505,420 + tig Sama_1223 0.34 -1.8 1,505,420 + tig Sama_1223 0.34 -2.5 1,505,420 + tig Sama_1223 0.34 -1.0 1,505,552 + tig Sama_1223 0.44 -0.7 1,505,634 + tig Sama_1223 0.50 -1.5 1,505,634 + tig Sama_1223 0.50 -3.2 1,505,716 + tig Sama_1223 0.57 -2.4 1,505,716 + tig Sama_1223 0.57 -1.2 1,505,770 + tig Sama_1223 0.61 -1.7 1,505,911 + tig Sama_1223 0.72 -1.8 1,505,911 + tig Sama_1223 0.72 -2.2 1,505,911 + tig Sama_1223 0.72 -1.5 1,506,044 + tig Sama_1223 0.82 +0.1 1,506,092 + tig Sama_1223 0.85 -2.4 1,506,092 + tig Sama_1223 0.85 -3.0 1,506,132 + tig Sama_1223 0.89 -2.7 1,506,135 + tig Sama_1223 0.89 -0.7 1,506,135 + tig Sama_1223 0.89 -2.7 1,506,254 + -2.0 1,506,254 + +0.5 1,506,314 + -1.0 1,506,314 + -0.5 1,506,326 + -1.2 1,506,326 + -1.0 1,506,353 + +0.3 1,506,449 + clpP Sama_1224 0.13 -0.7 1,506,449 + clpP Sama_1224 0.13 -1.3 1,506,450 - clpP Sama_1224 0.13 -1.3 1,506,450 - clpP Sama_1224 0.13 -2.7 1,506,458 + clpP Sama_1224 0.14 -0.7 1,506,458 + clpP Sama_1224 0.14 -0.5 1,506,458 + clpP Sama_1224 0.14 +0.0 1,506,458 + clpP Sama_1224 0.14 -1.2 1,506,458 + clpP Sama_1224 0.14 -0.2 1,506,458 + clpP Sama_1224 0.14 -1.1 1,506,459 - clpP Sama_1224 0.14 -1.8 1,506,459 - clpP Sama_1224 0.14 -2.0 1,506,578 + clpP Sama_1224 0.34 -1.1 1,506,578 + clpP Sama_1224 0.34 +0.3 1,506,578 + clpP Sama_1224 0.34 +0.3 1,506,579 - clpP Sama_1224 0.34 -1.5 1,506,584 + clpP Sama_1224 0.35 +0.7 1,506,585 - clpP Sama_1224 0.35 -2.1 1,506,585 - clpP Sama_1224 0.35 +0.3 1,506,629 + clpP Sama_1224 0.42 -1.1 1,506,634 + clpP Sama_1224 0.43 +0.3 1,506,637 + clpP Sama_1224 0.44 -1.0 1,506,637 + clpP Sama_1224 0.44 -0.2 1,506,637 + clpP Sama_1224 0.44 -1.2 1,506,637 + clpP Sama_1224 0.44 -0.4 1,506,637 + clpP Sama_1224 0.44 -0.2 1,506,638 - clpP Sama_1224 0.44 +0.0 1,506,691 + clpP Sama_1224 0.53 -1.8 1,506,691 + clpP Sama_1224 0.53 -1.5 1,506,859 - clpP Sama_1224 0.80 -1.7 1,506,944 + -1.5 1,507,023 + -1.8 1,507,104 + -1.8
Or see this region's nucleotide sequence