Experiment: Paraquat dichloride 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0515 and Sama_0516 are separated by 35 nucleotides Sama_0516 and Sama_0517 are separated by 126 nucleotides Sama_0517 and Sama_0518 are separated by 171 nucleotides Sama_0518 and Sama_0519 are separated by 9 nucleotides
Sama_0515: Sama_0515 - transporter, putative (RefSeq), at 634,698 to 636,065
_0515
Sama_0516: Sama_0516 - single-strand binding protein (RefSeq), at 636,101 to 636,805
_0516
Sama_0517: Sama_0517 - D-beta-hydroxybutyrate dehydrogenase (RefSeq), at 636,932 to 637,732
_0517
Sama_0518: Sama_0518 - 3-oxoacid CoA-transferase (RefSeq), at 637,904 to 638,665
_0518
Sama_0519: Sama_0519 - 3-oxoacid CoA-transferase (RefSeq), at 638,675 to 639,340
_0519
Position (kb) 636
637
638 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 635.962 kb on + strand at 635.963 kb on - strand at 636.000 kb on + strand at 636.028 kb on + strand at 636.810 kb on + strand at 636.819 kb on + strand at 636.819 kb on + strand at 636.819 kb on + strand at 636.820 kb on - strand at 636.820 kb on - strand at 636.820 kb on - strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.822 kb on - strand at 636.822 kb on - strand at 636.822 kb on - strand at 636.822 kb on - strand at 636.822 kb on - strand at 636.822 kb on - strand at 637.022 kb on + strand, within Sama_0517 at 637.022 kb on + strand, within Sama_0517 at 637.022 kb on + strand, within Sama_0517 at 637.077 kb on + strand, within Sama_0517 at 637.077 kb on + strand, within Sama_0517 at 637.078 kb on - strand, within Sama_0517 at 637.092 kb on + strand, within Sama_0517 at 637.093 kb on - strand, within Sama_0517 at 637.093 kb on - strand, within Sama_0517 at 637.105 kb on + strand, within Sama_0517 at 637.106 kb on - strand, within Sama_0517 at 637.106 kb on - strand, within Sama_0517 at 637.124 kb on + strand, within Sama_0517 at 637.124 kb on + strand, within Sama_0517 at 637.124 kb on + strand, within Sama_0517 at 637.124 kb on + strand, within Sama_0517 at 637.125 kb on - strand, within Sama_0517 at 637.125 kb on - strand, within Sama_0517 at 637.125 kb on - strand, within Sama_0517 at 637.125 kb on - strand, within Sama_0517 at 637.132 kb on + strand, within Sama_0517 at 637.132 kb on + strand, within Sama_0517 at 637.133 kb on - strand, within Sama_0517 at 637.133 kb on - strand, within Sama_0517 at 637.133 kb on - strand, within Sama_0517 at 637.133 kb on - strand, within Sama_0517 at 637.133 kb on - strand, within Sama_0517 at 637.147 kb on + strand, within Sama_0517 at 637.147 kb on + strand, within Sama_0517 at 637.180 kb on + strand, within Sama_0517 at 637.181 kb on - strand, within Sama_0517 at 637.231 kb on + strand, within Sama_0517 at 637.231 kb on + strand, within Sama_0517 at 637.231 kb on + strand, within Sama_0517 at 637.231 kb on + strand, within Sama_0517 at 637.231 kb on + strand, within Sama_0517 at 637.232 kb on - strand, within Sama_0517 at 637.232 kb on - strand, within Sama_0517 at 637.252 kb on - strand, within Sama_0517 at 637.297 kb on + strand, within Sama_0517 at 637.298 kb on - strand, within Sama_0517 at 637.298 kb on - strand, within Sama_0517 at 637.298 kb on - strand, within Sama_0517 at 637.453 kb on + strand, within Sama_0517 at 637.453 kb on + strand, within Sama_0517 at 637.454 kb on - strand, within Sama_0517 at 637.454 kb on - strand, within Sama_0517 at 637.495 kb on + strand, within Sama_0517 at 637.496 kb on - strand, within Sama_0517 at 637.523 kb on + strand, within Sama_0517 at 637.523 kb on + strand, within Sama_0517 at 637.523 kb on + strand, within Sama_0517 at 637.523 kb on + strand, within Sama_0517 at 637.524 kb on - strand, within Sama_0517 at 637.524 kb on - strand, within Sama_0517 at 637.524 kb on - strand, within Sama_0517 at 637.524 kb on - strand, within Sama_0517 at 637.524 kb on - strand, within Sama_0517 at 637.524 kb on - strand, within Sama_0517 at 637.557 kb on + strand, within Sama_0517 at 637.582 kb on + strand, within Sama_0517 at 637.583 kb on - strand, within Sama_0517 at 637.583 kb on - strand, within Sama_0517 at 637.619 kb on + strand, within Sama_0517 at 637.620 kb on - strand, within Sama_0517 at 637.647 kb on - strand, within Sama_0517 at 637.690 kb on + strand at 637.691 kb on - strand at 637.710 kb on - strand at 637.710 kb on - strand at 637.710 kb on - strand at 637.710 kb on - strand at 637.733 kb on + strand at 637.733 kb on + strand at 637.734 kb on - strand at 637.734 kb on - strand at 637.734 kb on - strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.867 kb on + strand at 637.867 kb on + strand at 637.868 kb on - strand at 637.868 kb on - strand at 637.868 kb on - strand at 637.868 kb on - strand at 637.868 kb on - strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.882 kb on + strand at 637.882 kb on + strand at 637.882 kb on + strand at 637.882 kb on + strand at 637.883 kb on - strand at 637.883 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.912 kb on + strand at 637.912 kb on + strand at 637.912 kb on + strand at 637.912 kb on + strand at 637.912 kb on + strand at 637.913 kb on - strand at 637.913 kb on - strand at 637.913 kb on - strand at 637.915 kb on + strand at 637.915 kb on + strand at 637.915 kb on + strand at 637.915 kb on + strand at 637.916 kb on - strand at 637.966 kb on + strand at 637.966 kb on + strand at 637.967 kb on - strand at 637.967 kb on - strand at 637.982 kb on + strand, within Sama_0518 at 637.982 kb on + strand, within Sama_0518 at 637.983 kb on - strand, within Sama_0518 at 637.983 kb on - strand, within Sama_0518 at 637.983 kb on - strand, within Sama_0518 at 637.983 kb on - strand, within Sama_0518 at 638.051 kb on - strand, within Sama_0518 at 638.135 kb on + strand, within Sama_0518 at 638.136 kb on - strand, within Sama_0518 at 638.136 kb on - strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.203 kb on + strand, within Sama_0518 at 638.299 kb on + strand, within Sama_0518 at 638.301 kb on + strand, within Sama_0518 at 638.313 kb on - strand, within Sama_0518 at 638.382 kb on - strand, within Sama_0518 at 638.382 kb on - strand, within Sama_0518 at 638.441 kb on - strand, within Sama_0518 at 638.444 kb on + strand, within Sama_0518 at 638.445 kb on - strand, within Sama_0518 at 638.457 kb on + strand, within Sama_0518 at 638.458 kb on - strand, within Sama_0518 at 638.511 kb on + strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.585 kb on + strand, within Sama_0518 at 638.586 kb on - strand, within Sama_0518 at 638.586 kb on - strand, within Sama_0518 at 638.586 kb on - strand, within Sama_0518 at 638.659 kb on + strand at 638.659 kb on + strand at 638.659 kb on + strand at 638.660 kb on - strand at 638.663 kb on + strand at 638.663 kb on + strand at 638.664 kb on - strand at 638.664 kb on - strand at 638.664 kb on - strand at 638.732 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.03125 mg/ml remove 635,962 + -2.1 635,963 - +0.8 636,000 + -0.7 636,028 + +0.7 636,810 + +0.1 636,819 + -0.2 636,819 + +1.7 636,819 + -0.5 636,820 - +0.3 636,820 - -0.0 636,820 - -1.7 636,821 + -1.1 636,821 + +0.4 636,821 + -0.5 636,821 + +0.8 636,821 + -0.8 636,821 + +0.7 636,821 + +0.2 636,821 + -1.0 636,821 + +1.2 636,821 + +0.3 636,821 + +0.1 636,821 + -0.3 636,821 + +0.1 636,821 + +0.1 636,821 + -0.6 636,821 + -1.0 636,821 + -1.8 636,821 + -0.5 636,822 - -0.7 636,822 - +1.2 636,822 - -0.1 636,822 - -2.2 636,822 - +0.3 636,822 - -2.1 637,022 + Sama_0517 0.11 +0.7 637,022 + Sama_0517 0.11 +0.1 637,022 + Sama_0517 0.11 +0.2 637,077 + Sama_0517 0.18 -2.0 637,077 + Sama_0517 0.18 -1.7 637,078 - Sama_0517 0.18 -0.2 637,092 + Sama_0517 0.20 -0.8 637,093 - Sama_0517 0.20 +0.9 637,093 - Sama_0517 0.20 +0.2 637,105 + Sama_0517 0.22 -1.1 637,106 - Sama_0517 0.22 -0.3 637,106 - Sama_0517 0.22 -0.8 637,124 + Sama_0517 0.24 -0.7 637,124 + Sama_0517 0.24 +0.5 637,124 + Sama_0517 0.24 +0.7 637,124 + Sama_0517 0.24 -0.3 637,125 - Sama_0517 0.24 +0.2 637,125 - Sama_0517 0.24 -0.9 637,125 - Sama_0517 0.24 -0.5 637,125 - Sama_0517 0.24 +0.9 637,132 + Sama_0517 0.25 +0.3 637,132 + Sama_0517 0.25 +0.5 637,133 - Sama_0517 0.25 +0.2 637,133 - Sama_0517 0.25 +1.8 637,133 - Sama_0517 0.25 +0.3 637,133 - Sama_0517 0.25 +0.1 637,133 - Sama_0517 0.25 -1.1 637,147 + Sama_0517 0.27 +0.3 637,147 + Sama_0517 0.27 -0.8 637,180 + Sama_0517 0.31 -1.3 637,181 - Sama_0517 0.31 -2.3 637,231 + Sama_0517 0.37 +0.2 637,231 + Sama_0517 0.37 +0.5 637,231 + Sama_0517 0.37 -1.5 637,231 + Sama_0517 0.37 +0.0 637,231 + Sama_0517 0.37 +1.0 637,232 - Sama_0517 0.37 +1.1 637,232 - Sama_0517 0.37 -0.3 637,252 - Sama_0517 0.40 -0.7 637,297 + Sama_0517 0.46 +0.6 637,298 - Sama_0517 0.46 +0.4 637,298 - Sama_0517 0.46 -0.6 637,298 - Sama_0517 0.46 +1.1 637,453 + Sama_0517 0.65 -0.5 637,453 + Sama_0517 0.65 +0.1 637,454 - Sama_0517 0.65 +0.7 637,454 - Sama_0517 0.65 +0.5 637,495 + Sama_0517 0.70 -0.3 637,496 - Sama_0517 0.70 +0.5 637,523 + Sama_0517 0.74 +2.0 637,523 + Sama_0517 0.74 +0.5 637,523 + Sama_0517 0.74 +0.2 637,523 + Sama_0517 0.74 -0.8 637,524 - Sama_0517 0.74 +0.4 637,524 - Sama_0517 0.74 -0.3 637,524 - Sama_0517 0.74 -3.1 637,524 - Sama_0517 0.74 -1.0 637,524 - Sama_0517 0.74 -1.3 637,524 - Sama_0517 0.74 +0.3 637,557 + Sama_0517 0.78 -0.0 637,582 + Sama_0517 0.81 -0.7 637,583 - Sama_0517 0.81 +1.6 637,583 - Sama_0517 0.81 -0.3 637,619 + Sama_0517 0.86 -0.1 637,620 - Sama_0517 0.86 -0.4 637,647 - Sama_0517 0.89 +0.3 637,690 + +0.4 637,691 - +0.5 637,710 - -1.5 637,710 - +0.1 637,710 - -0.2 637,710 - -0.3 637,733 + -0.5 637,733 + +0.0 637,734 - -0.7 637,734 - -0.5 637,734 - +0.7 637,762 + +1.5 637,762 + -1.3 637,762 + -2.6 637,762 + -0.5 637,762 + +0.3 637,762 + +1.7 637,762 + +1.0 637,762 + -2.5 637,762 + +0.3 637,762 + -0.1 637,763 - -0.9 637,763 - +1.2 637,763 - -0.7 637,763 - -0.1 637,763 - +1.8 637,763 - -0.6 637,763 - -1.7 637,763 - -0.6 637,763 - +0.3 637,763 - +0.2 637,763 - -0.5 637,763 - -1.6 637,867 + +0.7 637,867 + -1.3 637,868 - -2.0 637,868 - -0.6 637,868 - +0.4 637,868 - +0.7 637,868 - -2.3 637,879 + -0.0 637,879 + -2.6 637,879 + -0.7 637,879 + -0.5 637,879 + -1.0 637,879 + +0.0 637,879 + -1.8 637,879 + +1.1 637,879 + -3.0 637,879 + +1.2 637,880 - +0.7 637,880 - +1.8 637,880 - +0.1 637,880 - -1.7 637,880 - -0.6 637,880 - +2.3 637,880 - -1.3 637,880 - -0.1 637,880 - -0.1 637,880 - +0.5 637,882 + -1.3 637,882 + -0.1 637,882 + +1.9 637,882 + +0.3 637,883 - +0.1 637,883 - -1.3 637,888 - -0.2 637,888 - -0.5 637,888 - +2.1 637,888 - -1.9 637,888 - -0.7 637,888 - +2.5 637,912 + -0.5 637,912 + +0.5 637,912 + -0.6 637,912 + +1.1 637,912 + +0.3 637,913 - -0.2 637,913 - -1.3 637,913 - -0.9 637,915 + +0.1 637,915 + -1.4 637,915 + -1.0 637,915 + +0.2 637,916 - +1.5 637,966 + -1.0 637,966 + +0.2 637,967 - +0.3 637,967 - +0.1 637,982 + Sama_0518 0.10 -1.2 637,982 + Sama_0518 0.10 +0.5 637,983 - Sama_0518 0.10 -0.2 637,983 - Sama_0518 0.10 -0.1 637,983 - Sama_0518 0.10 -0.2 637,983 - Sama_0518 0.10 -0.7 638,051 - Sama_0518 0.19 +1.7 638,135 + Sama_0518 0.30 -0.1 638,136 - Sama_0518 0.30 +1.9 638,136 - Sama_0518 0.30 -0.5 638,189 + Sama_0518 0.37 +0.8 638,189 + Sama_0518 0.37 -0.5 638,189 + Sama_0518 0.37 -0.1 638,189 + Sama_0518 0.37 +0.3 638,189 + Sama_0518 0.37 -0.3 638,190 - Sama_0518 0.38 -0.8 638,190 - Sama_0518 0.38 -0.6 638,190 - Sama_0518 0.38 -1.2 638,190 - Sama_0518 0.38 -1.1 638,190 - Sama_0518 0.38 -0.2 638,190 - Sama_0518 0.38 +0.2 638,203 + Sama_0518 0.39 +1.0 638,299 + Sama_0518 0.52 +0.7 638,301 + Sama_0518 0.52 +0.7 638,313 - Sama_0518 0.54 -0.6 638,382 - Sama_0518 0.63 +0.5 638,382 - Sama_0518 0.63 +0.0 638,441 - Sama_0518 0.70 +0.3 638,444 + Sama_0518 0.71 -0.3 638,445 - Sama_0518 0.71 -1.5 638,457 + Sama_0518 0.73 +0.1 638,458 - Sama_0518 0.73 -0.9 638,511 + Sama_0518 0.80 +0.1 638,512 - Sama_0518 0.80 -0.2 638,512 - Sama_0518 0.80 -1.4 638,512 - Sama_0518 0.80 +0.4 638,512 - Sama_0518 0.80 -0.8 638,512 - Sama_0518 0.80 -1.8 638,585 + Sama_0518 0.89 +0.0 638,586 - Sama_0518 0.90 +1.0 638,586 - Sama_0518 0.90 -1.2 638,586 - Sama_0518 0.90 -2.4 638,659 + +0.5 638,659 + +0.5 638,659 + -0.8 638,660 - -0.3 638,663 + -0.8 638,663 + -0.8 638,664 - +0.1 638,664 - -2.4 638,664 - -0.6 638,732 + -0.2
Or see this region's nucleotide sequence