Experiment: Paraquat dichloride 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0243 and Sama_0244 are separated by 1 nucleotides Sama_0244 and Sama_0245 are separated by 11 nucleotides Sama_0245 and Sama_0246 are separated by 310 nucleotides Sama_0246 and Sama_0247 are separated by 148 nucleotides
Sama_0243: Sama_0243 - heme exporter protein CcmC (RefSeq), at 267,296 to 268,042
_0243
Sama_0244: Sama_0244 - heme exporter protein CcmB (RefSeq), at 268,044 to 268,730
_0244
Sama_0245: Sama_0245 - cytochrome c biogenesis protein CcmA (RefSeq), at 268,742 to 269,395
_0245
Sama_0246: Sama_0246 - cytochrome c (RefSeq), at 269,706 to 269,999
_0246
Sama_0247: Sama_0247 - cytochrome c-type biogenesis protein CcmH (RefSeq), at 270,148 to 271,401
_0247
Position (kb) 268
269
270 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 267.784 kb on + strand, within Sama_0243 at 267.805 kb on + strand, within Sama_0243 at 267.806 kb on - strand, within Sama_0243 at 267.829 kb on + strand, within Sama_0243 at 267.829 kb on + strand, within Sama_0243 at 267.830 kb on - strand, within Sama_0243 at 267.831 kb on + strand, within Sama_0243 at 267.831 kb on + strand, within Sama_0243 at 267.831 kb on + strand, within Sama_0243 at 267.831 kb on + strand, within Sama_0243 at 267.831 kb on + strand, within Sama_0243 at 267.831 kb on + strand, within Sama_0243 at 267.831 kb on + strand, within Sama_0243 at 267.832 kb on - strand, within Sama_0243 at 267.832 kb on - strand, within Sama_0243 at 267.868 kb on + strand, within Sama_0243 at 267.900 kb on + strand, within Sama_0243 at 267.901 kb on - strand, within Sama_0243 at 267.901 kb on - strand, within Sama_0243 at 267.901 kb on - strand, within Sama_0243 at 267.946 kb on + strand, within Sama_0243 at 267.978 kb on - strand at 267.978 kb on - strand at 267.992 kb on + strand at 268.000 kb on - strand at 268.028 kb on + strand at 268.029 kb on - strand at 268.043 kb on - strand at 268.045 kb on + strand at 268.045 kb on + strand at 268.046 kb on - strand at 268.046 kb on - strand at 268.049 kb on + strand at 268.050 kb on - strand at 268.050 kb on - strand at 268.119 kb on + strand, within Sama_0244 at 268.119 kb on + strand, within Sama_0244 at 268.120 kb on - strand, within Sama_0244 at 268.120 kb on - strand, within Sama_0244 at 268.120 kb on - strand, within Sama_0244 at 268.122 kb on + strand, within Sama_0244 at 268.142 kb on - strand, within Sama_0244 at 268.142 kb on - strand, within Sama_0244 at 268.147 kb on - strand, within Sama_0244 at 268.201 kb on + strand, within Sama_0244 at 268.202 kb on - strand, within Sama_0244 at 268.202 kb on - strand, within Sama_0244 at 268.202 kb on - strand, within Sama_0244 at 268.212 kb on + strand, within Sama_0244 at 268.213 kb on - strand, within Sama_0244 at 268.215 kb on - strand, within Sama_0244 at 268.216 kb on - strand, within Sama_0244 at 268.231 kb on - strand, within Sama_0244 at 268.241 kb on + strand, within Sama_0244 at 268.242 kb on - strand, within Sama_0244 at 268.242 kb on - strand, within Sama_0244 at 268.245 kb on + strand, within Sama_0244 at 268.245 kb on + strand, within Sama_0244 at 268.245 kb on + strand, within Sama_0244 at 268.246 kb on - strand, within Sama_0244 at 268.246 kb on - strand, within Sama_0244 at 268.269 kb on + strand, within Sama_0244 at 268.269 kb on + strand, within Sama_0244 at 268.270 kb on - strand, within Sama_0244 at 268.270 kb on - strand, within Sama_0244 at 268.270 kb on - strand, within Sama_0244 at 268.270 kb on - strand, within Sama_0244 at 268.270 kb on - strand, within Sama_0244 at 268.298 kb on + strand, within Sama_0244 at 268.330 kb on + strand, within Sama_0244 at 268.330 kb on + strand, within Sama_0244 at 268.331 kb on - strand, within Sama_0244 at 268.331 kb on - strand, within Sama_0244 at 268.331 kb on - strand, within Sama_0244 at 268.439 kb on - strand, within Sama_0244 at 268.439 kb on - strand, within Sama_0244 at 268.456 kb on - strand, within Sama_0244 at 268.539 kb on + strand, within Sama_0244 at 268.573 kb on - strand, within Sama_0244 at 268.573 kb on - strand, within Sama_0244 at 268.689 kb on + strand at 268.689 kb on + strand at 268.690 kb on - strand at 268.709 kb on + strand at 268.715 kb on + strand at 268.716 kb on - strand at 268.716 kb on - strand at 268.716 kb on - strand at 268.744 kb on - strand at 268.744 kb on - strand at 268.745 kb on + strand at 268.745 kb on + strand at 268.745 kb on + strand at 268.745 kb on + strand at 268.745 kb on + strand at 268.745 kb on + strand at 268.745 kb on + strand at 268.746 kb on - strand at 268.746 kb on - strand at 268.746 kb on - strand at 268.746 kb on - strand at 268.746 kb on - strand at 268.746 kb on - strand at 268.755 kb on + strand at 268.756 kb on - strand at 268.897 kb on + strand, within Sama_0245 at 268.980 kb on - strand, within Sama_0245 at 269.055 kb on + strand, within Sama_0245 at 269.070 kb on - strand, within Sama_0245 at 269.081 kb on - strand, within Sama_0245 at 269.133 kb on + strand, within Sama_0245 at 269.133 kb on + strand, within Sama_0245 at 269.134 kb on - strand, within Sama_0245 at 269.138 kb on + strand, within Sama_0245 at 269.138 kb on + strand, within Sama_0245 at 269.139 kb on - strand, within Sama_0245 at 269.139 kb on - strand, within Sama_0245 at 269.139 kb on - strand, within Sama_0245 at 269.139 kb on - strand, within Sama_0245 at 269.282 kb on - strand, within Sama_0245 at 269.361 kb on - strand at 269.387 kb on + strand at 269.388 kb on - strand at 269.405 kb on + strand at 269.405 kb on + strand at 269.406 kb on - strand at 269.406 kb on - strand at 269.406 kb on - strand at 269.406 kb on - strand at 269.416 kb on - strand at 269.430 kb on - strand at 269.432 kb on + strand at 269.433 kb on - strand at 269.433 kb on - strand at 269.433 kb on - strand at 269.433 kb on - strand at 269.433 kb on - strand at 269.433 kb on - strand at 269.433 kb on - strand at 269.437 kb on + strand at 269.437 kb on + strand at 269.438 kb on - strand at 269.438 kb on - strand at 269.438 kb on - strand at 269.438 kb on - strand at 269.438 kb on - strand at 269.438 kb on - strand at 269.438 kb on - strand at 269.438 kb on - strand at 269.456 kb on - strand at 269.466 kb on + strand at 269.466 kb on + strand at 269.466 kb on + strand at 269.467 kb on - strand at 269.467 kb on - strand at 269.467 kb on - strand at 269.471 kb on + strand at 269.471 kb on + strand at 269.471 kb on + strand at 269.472 kb on - strand at 269.472 kb on - strand at 269.472 kb on - strand at 269.472 kb on - strand at 269.475 kb on + strand at 269.475 kb on + strand at 269.476 kb on - strand at 269.476 kb on - strand at 269.500 kb on + strand at 269.501 kb on - strand at 269.501 kb on - strand at 269.501 kb on - strand at 269.529 kb on + strand at 269.539 kb on + strand at 269.540 kb on - strand at 269.541 kb on + strand at 269.542 kb on - strand at 269.542 kb on - strand at 269.542 kb on - strand at 269.560 kb on + strand at 269.560 kb on + strand at 269.625 kb on + strand at 269.625 kb on + strand at 269.625 kb on + strand at 269.626 kb on - strand at 269.626 kb on - strand at 269.633 kb on + strand at 269.642 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.680 kb on - strand at 269.680 kb on - strand at 269.680 kb on - strand at 269.680 kb on - strand at 269.680 kb on - strand at 269.680 kb on - strand at 269.680 kb on - strand at 269.680 kb on - strand at 269.680 kb on - strand at 269.683 kb on + strand at 269.683 kb on + strand at 269.684 kb on - strand at 269.684 kb on - strand at 269.693 kb on - strand at 269.720 kb on - strand at 269.720 kb on - strand at 269.734 kb on + strand at 269.734 kb on + strand at 269.734 kb on + strand at 269.734 kb on + strand at 269.735 kb on - strand at 269.735 kb on - strand at 269.735 kb on - strand at 269.735 kb on - strand at 269.747 kb on + strand, within Sama_0246 at 269.747 kb on + strand, within Sama_0246 at 269.762 kb on - strand, within Sama_0246 at 269.785 kb on + strand, within Sama_0246 at 269.785 kb on + strand, within Sama_0246 at 269.785 kb on + strand, within Sama_0246 at 269.786 kb on - strand, within Sama_0246 at 269.786 kb on - strand, within Sama_0246 at 269.786 kb on - strand, within Sama_0246 at 269.786 kb on - strand, within Sama_0246 at 269.786 kb on - strand, within Sama_0246 at 269.787 kb on + strand, within Sama_0246 at 269.787 kb on + strand, within Sama_0246 at 269.787 kb on + strand, within Sama_0246 at 269.787 kb on + strand, within Sama_0246 at 269.787 kb on + strand, within Sama_0246 at 269.787 kb on + strand, within Sama_0246 at 269.788 kb on - strand, within Sama_0246 at 269.788 kb on - strand, within Sama_0246 at 269.789 kb on + strand, within Sama_0246 at 269.789 kb on + strand, within Sama_0246 at 269.789 kb on + strand, within Sama_0246 at 269.789 kb on + strand, within Sama_0246 at 269.789 kb on + strand, within Sama_0246 at 269.789 kb on + strand, within Sama_0246 at 269.789 kb on + strand, within Sama_0246 at 269.789 kb on + strand, within Sama_0246 at 269.789 kb on + strand, within Sama_0246 at 269.789 kb on + strand, within Sama_0246 at 269.790 kb on - strand, within Sama_0246 at 269.790 kb on - strand, within Sama_0246 at 269.790 kb on - strand, within Sama_0246 at 269.790 kb on - strand, within Sama_0246 at 269.790 kb on - strand, within Sama_0246 at 269.790 kb on - strand, within Sama_0246 at 269.806 kb on + strand, within Sama_0246 at 269.806 kb on + strand, within Sama_0246 at 269.807 kb on - strand, within Sama_0246 at 269.807 kb on - strand, within Sama_0246 at 269.807 kb on - strand, within Sama_0246 at 269.807 kb on - strand, within Sama_0246 at 269.807 kb on - strand, within Sama_0246 at 269.824 kb on + strand, within Sama_0246 at 269.882 kb on + strand, within Sama_0246 at 269.882 kb on + strand, within Sama_0246 at 269.955 kb on - strand, within Sama_0246 at 270.008 kb on + strand at 270.008 kb on + strand at 270.008 kb on + strand at 270.008 kb on + strand at 270.009 kb on - strand at 270.009 kb on - strand at 270.042 kb on - strand at 270.118 kb on + strand at 270.118 kb on + strand at 270.123 kb on + strand at 270.124 kb on - strand at 270.131 kb on + strand at 270.131 kb on + strand at 270.131 kb on + strand at 270.132 kb on - strand at 270.149 kb on + strand at 270.150 kb on - strand at 270.170 kb on - strand at 270.170 kb on - strand at 270.207 kb on + strand at 270.207 kb on + strand at 270.207 kb on + strand at 270.208 kb on - strand at 270.208 kb on - strand at 270.219 kb on + strand at 270.220 kb on - strand at 270.220 kb on - strand at 270.220 kb on + strand at 270.241 kb on - strand at 270.315 kb on + strand, within Sama_0247 at 270.316 kb on - strand, within Sama_0247 at 270.316 kb on - strand, within Sama_0247 at 270.324 kb on + strand, within Sama_0247 at 270.324 kb on + strand, within Sama_0247 at 270.325 kb on - strand, within Sama_0247 at 270.325 kb on - strand, within Sama_0247 at 270.325 kb on - strand, within Sama_0247 at 270.339 kb on + strand, within Sama_0247 at 270.340 kb on - strand, within Sama_0247 at 270.340 kb on - strand, within Sama_0247 at 270.340 kb on - strand, within Sama_0247
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.03125 mg/ml remove 267,784 + Sama_0243 0.65 -0.7 267,805 + Sama_0243 0.68 -1.2 267,806 - Sama_0243 0.68 -1.5 267,829 + Sama_0243 0.71 -1.0 267,829 + Sama_0243 0.71 +0.8 267,830 - Sama_0243 0.71 +0.0 267,831 + Sama_0243 0.72 -0.7 267,831 + Sama_0243 0.72 -0.2 267,831 + Sama_0243 0.72 -0.5 267,831 + Sama_0243 0.72 -0.5 267,831 + Sama_0243 0.72 -1.0 267,831 + Sama_0243 0.72 -0.2 267,831 + Sama_0243 0.72 -0.5 267,832 - Sama_0243 0.72 -0.5 267,832 - Sama_0243 0.72 -0.5 267,868 + Sama_0243 0.77 -1.0 267,900 + Sama_0243 0.81 -1.0 267,901 - Sama_0243 0.81 -0.5 267,901 - Sama_0243 0.81 -2.2 267,901 - Sama_0243 0.81 -0.5 267,946 + Sama_0243 0.87 -1.3 267,978 - -0.5 267,978 - -1.2 267,992 + -1.0 268,000 - -2.0 268,028 + -0.2 268,029 - -0.5 268,043 - -1.2 268,045 + -0.5 268,045 + -2.1 268,046 - -1.0 268,046 - -2.2 268,049 + -0.2 268,050 - -1.6 268,050 - -1.3 268,119 + Sama_0244 0.11 +0.0 268,119 + Sama_0244 0.11 -0.2 268,120 - Sama_0244 0.11 -1.0 268,120 - Sama_0244 0.11 -0.2 268,120 - Sama_0244 0.11 +0.6 268,122 + Sama_0244 0.11 -0.2 268,142 - Sama_0244 0.14 -0.7 268,142 - Sama_0244 0.14 +1.8 268,147 - Sama_0244 0.15 +0.3 268,201 + Sama_0244 0.23 -1.2 268,202 - Sama_0244 0.23 -1.0 268,202 - Sama_0244 0.23 -1.5 268,202 - Sama_0244 0.23 -1.6 268,212 + Sama_0244 0.24 -1.2 268,213 - Sama_0244 0.25 -0.5 268,215 - Sama_0244 0.25 -0.7 268,216 - Sama_0244 0.25 -1.0 268,231 - Sama_0244 0.27 -0.7 268,241 + Sama_0244 0.29 -1.0 268,242 - Sama_0244 0.29 -0.2 268,242 - Sama_0244 0.29 -1.0 268,245 + Sama_0244 0.29 +0.8 268,245 + Sama_0244 0.29 -1.2 268,245 + Sama_0244 0.29 +0.3 268,246 - Sama_0244 0.29 -0.7 268,246 - Sama_0244 0.29 -1.3 268,269 + Sama_0244 0.33 +0.3 268,269 + Sama_0244 0.33 -0.2 268,270 - Sama_0244 0.33 -0.5 268,270 - Sama_0244 0.33 -0.7 268,270 - Sama_0244 0.33 -1.2 268,270 - Sama_0244 0.33 -1.2 268,270 - Sama_0244 0.33 -2.4 268,298 + Sama_0244 0.37 -0.2 268,330 + Sama_0244 0.42 -1.0 268,330 + Sama_0244 0.42 -1.9 268,331 - Sama_0244 0.42 -1.2 268,331 - Sama_0244 0.42 -2.0 268,331 - Sama_0244 0.42 -1.2 268,439 - Sama_0244 0.57 -1.6 268,439 - Sama_0244 0.57 -0.2 268,456 - Sama_0244 0.60 -0.5 268,539 + Sama_0244 0.72 -1.0 268,573 - Sama_0244 0.77 -2.3 268,573 - Sama_0244 0.77 -1.7 268,689 + -0.2 268,689 + -1.6 268,690 - -0.2 268,709 + -1.6 268,715 + -1.2 268,716 - +0.3 268,716 - -1.3 268,716 - -0.7 268,744 - -1.6 268,744 - -1.4 268,745 + -2.7 268,745 + -2.2 268,745 + -1.7 268,745 + -0.7 268,745 + -0.2 268,745 + -0.5 268,745 + -1.5 268,746 - -1.6 268,746 - -1.0 268,746 - -1.2 268,746 - -0.7 268,746 - -1.6 268,746 - -1.5 268,755 + -0.5 268,756 - -1.5 268,897 + Sama_0245 0.24 +0.3 268,980 - Sama_0245 0.36 -0.5 269,055 + Sama_0245 0.48 -3.6 269,070 - Sama_0245 0.50 -0.7 269,081 - Sama_0245 0.52 -1.2 269,133 + Sama_0245 0.60 -1.2 269,133 + Sama_0245 0.60 -1.0 269,134 - Sama_0245 0.60 -1.5 269,138 + Sama_0245 0.61 -0.5 269,138 + Sama_0245 0.61 -1.7 269,139 - Sama_0245 0.61 -0.2 269,139 - Sama_0245 0.61 -1.2 269,139 - Sama_0245 0.61 -1.6 269,139 - Sama_0245 0.61 -1.3 269,282 - Sama_0245 0.83 -1.2 269,361 - +0.1 269,387 + -1.7 269,388 - -0.7 269,405 + -1.0 269,405 + -1.5 269,406 - +1.0 269,406 - -1.9 269,406 - -2.0 269,406 - +0.0 269,416 - +0.1 269,430 - -0.7 269,432 + -2.3 269,433 - +0.5 269,433 - -0.5 269,433 - -2.4 269,433 - +0.1 269,433 - -0.7 269,433 - +0.3 269,433 - +0.8 269,437 + -1.3 269,437 + +0.8 269,438 - -1.1 269,438 - +1.0 269,438 - -0.9 269,438 - +0.6 269,438 - +0.7 269,438 - -0.3 269,438 - +0.3 269,438 - -2.0 269,456 - -0.8 269,466 + +0.3 269,466 + -1.9 269,466 + -0.9 269,467 - +0.5 269,467 - -2.3 269,467 - +0.8 269,471 + +0.3 269,471 + +0.6 269,471 + +0.5 269,472 - -0.1 269,472 - +0.2 269,472 - +1.4 269,472 - -0.3 269,475 + +0.5 269,475 + +0.6 269,476 - -0.3 269,476 - -0.0 269,500 + -0.6 269,501 - -0.9 269,501 - +0.0 269,501 - -1.0 269,529 + +0.3 269,539 + +0.1 269,540 - -0.5 269,541 + -2.4 269,542 - -0.6 269,542 - -0.3 269,542 - -0.7 269,560 + -0.9 269,560 + +0.8 269,625 + -0.4 269,625 + +0.1 269,625 + -0.3 269,626 - -2.2 269,626 - -1.0 269,633 + -1.1 269,642 + -0.7 269,679 + -0.7 269,679 + +0.5 269,679 + -0.6 269,679 + -0.1 269,679 + -0.2 269,679 + -1.0 269,679 + -0.7 269,679 + -0.8 269,679 + +0.6 269,679 + +0.6 269,679 + -0.2 269,679 + +1.0 269,680 - -1.6 269,680 - -1.0 269,680 - -1.0 269,680 - -1.5 269,680 - -2.3 269,680 - -1.2 269,680 - -1.6 269,680 - -0.7 269,680 - -1.0 269,683 + -1.5 269,683 + -2.0 269,684 - -2.3 269,684 - -1.7 269,693 - -2.5 269,720 - -2.2 269,720 - -1.7 269,734 + -2.9 269,734 + -0.7 269,734 + -2.3 269,734 + -0.7 269,735 - -1.0 269,735 - -0.2 269,735 - +0.5 269,735 - -1.3 269,747 + Sama_0246 0.14 -1.0 269,747 + Sama_0246 0.14 -3.2 269,762 - Sama_0246 0.19 -1.2 269,785 + Sama_0246 0.27 -1.0 269,785 + Sama_0246 0.27 -1.5 269,785 + Sama_0246 0.27 -0.7 269,786 - Sama_0246 0.27 -1.6 269,786 - Sama_0246 0.27 -3.1 269,786 - Sama_0246 0.27 -0.5 269,786 - Sama_0246 0.27 -1.2 269,786 - Sama_0246 0.27 -1.6 269,787 + Sama_0246 0.28 -2.7 269,787 + Sama_0246 0.28 -1.6 269,787 + Sama_0246 0.28 -1.4 269,787 + Sama_0246 0.28 -0.9 269,787 + Sama_0246 0.28 -1.0 269,787 + Sama_0246 0.28 -2.3 269,788 - Sama_0246 0.28 -1.9 269,788 - Sama_0246 0.28 +0.3 269,789 + Sama_0246 0.28 -2.5 269,789 + Sama_0246 0.28 -1.5 269,789 + Sama_0246 0.28 -1.3 269,789 + Sama_0246 0.28 -1.9 269,789 + Sama_0246 0.28 -1.9 269,789 + Sama_0246 0.28 -0.2 269,789 + Sama_0246 0.28 -1.0 269,789 + Sama_0246 0.28 -0.2 269,789 + Sama_0246 0.28 -1.0 269,789 + Sama_0246 0.28 -2.2 269,790 - Sama_0246 0.29 +0.3 269,790 - Sama_0246 0.29 -0.7 269,790 - Sama_0246 0.29 +0.5 269,790 - Sama_0246 0.29 -1.0 269,790 - Sama_0246 0.29 -0.5 269,790 - Sama_0246 0.29 -2.3 269,806 + Sama_0246 0.34 -1.6 269,806 + Sama_0246 0.34 +0.3 269,807 - Sama_0246 0.34 -1.0 269,807 - Sama_0246 0.34 +0.3 269,807 - Sama_0246 0.34 -0.7 269,807 - Sama_0246 0.34 -0.5 269,807 - Sama_0246 0.34 -1.3 269,824 + Sama_0246 0.40 -2.4 269,882 + Sama_0246 0.60 -1.2 269,882 + Sama_0246 0.60 -1.2 269,955 - Sama_0246 0.85 -0.9 270,008 + -1.1 270,008 + -0.7 270,008 + -1.3 270,008 + -0.1 270,009 - +0.4 270,009 - +0.0 270,042 - -2.6 270,118 + +0.6 270,118 + -0.6 270,123 + -0.5 270,124 - +0.6 270,131 + -0.5 270,131 + -1.3 270,131 + +2.3 270,132 - -1.3 270,149 + -1.0 270,150 - -0.6 270,170 - -1.0 270,170 - +0.1 270,207 + -1.0 270,207 + -0.3 270,207 + -1.7 270,208 - +0.5 270,208 - +1.0 270,219 + -2.2 270,220 - -0.5 270,220 - -1.5 270,220 + -0.6 270,241 - -0.7 270,315 + Sama_0247 0.13 -0.6 270,316 - Sama_0247 0.13 -1.2 270,316 - Sama_0247 0.13 +0.8 270,324 + Sama_0247 0.14 -0.0 270,324 + Sama_0247 0.14 -2.1 270,325 - Sama_0247 0.14 +1.0 270,325 - Sama_0247 0.14 -0.6 270,325 - Sama_0247 0.14 +0.5 270,339 + Sama_0247 0.15 +0.8 270,340 - Sama_0247 0.15 +0.1 270,340 - Sama_0247 0.15 +3.0 270,340 - Sama_0247 0.15 -0.3
Or see this region's nucleotide sequence