Experiment: Bacitracin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glyA and nrdR are separated by 103 nucleotides nrdR and Sama_1016 are separated by 21 nucleotides
Sama_1014: glyA - serine hydroxymethyltransferase (RefSeq), at 1,229,015 to 1,230,268
glyA
Sama_1015: nrdR - transcriptional regulator NrdR (RefSeq), at 1,230,372 to 1,230,821
nrdR
Sama_1016: Sama_1016 - diaminohydroxyphosphoribosylaminopyrimidine deaminase., 5-amino-6-(5-phosphoribosylamino)uracil reductase (RefSeq), at 1,230,843 to 1,231,979
_1016
Position (kb)
1230
1231 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1229.413 kb on - strand, within glyA at 1229.432 kb on + strand, within glyA at 1229.432 kb on + strand, within glyA at 1229.432 kb on + strand, within glyA at 1229.432 kb on + strand, within glyA at 1229.432 kb on + strand, within glyA at 1229.433 kb on - strand, within glyA at 1229.433 kb on - strand, within glyA at 1229.433 kb on - strand, within glyA at 1229.447 kb on + strand, within glyA at 1229.447 kb on + strand, within glyA at 1229.477 kb on + strand, within glyA at 1229.544 kb on - strand, within glyA at 1229.600 kb on + strand, within glyA at 1229.600 kb on + strand, within glyA at 1229.600 kb on + strand, within glyA at 1229.600 kb on + strand, within glyA at 1229.600 kb on + strand, within glyA at 1229.601 kb on - strand, within glyA at 1229.601 kb on - strand, within glyA at 1229.601 kb on - strand, within glyA at 1229.625 kb on + strand, within glyA at 1229.625 kb on + strand, within glyA at 1229.625 kb on + strand, within glyA at 1229.626 kb on - strand, within glyA at 1229.649 kb on + strand, within glyA at 1229.649 kb on + strand, within glyA at 1229.649 kb on + strand, within glyA at 1229.651 kb on + strand, within glyA at 1229.680 kb on - strand, within glyA at 1229.759 kb on + strand, within glyA at 1229.759 kb on + strand, within glyA at 1229.760 kb on - strand, within glyA at 1229.781 kb on + strand, within glyA at 1229.873 kb on + strand, within glyA at 1229.873 kb on + strand, within glyA at 1229.873 kb on + strand, within glyA at 1229.873 kb on + strand, within glyA at 1229.916 kb on + strand, within glyA at 1229.916 kb on + strand, within glyA at 1229.916 kb on + strand, within glyA at 1229.917 kb on - strand, within glyA at 1229.917 kb on - strand, within glyA at 1229.917 kb on - strand, within glyA at 1229.917 kb on - strand, within glyA at 1230.004 kb on + strand, within glyA at 1230.049 kb on + strand, within glyA at 1230.049 kb on + strand, within glyA at 1230.049 kb on + strand, within glyA at 1230.049 kb on + strand, within glyA at 1230.181 kb on - strand at 1230.181 kb on - strand at 1230.181 kb on - strand at 1230.181 kb on - strand at 1230.181 kb on - strand at 1230.231 kb on + strand at 1230.231 kb on + strand at 1230.260 kb on + strand at 1230.261 kb on - strand at 1230.261 kb on - strand at 1230.274 kb on + strand at 1230.283 kb on + strand at 1230.283 kb on + strand at 1230.288 kb on + strand at 1230.288 kb on + strand at 1230.288 kb on + strand at 1230.288 kb on + strand at 1230.321 kb on + strand at 1230.327 kb on + strand at 1230.327 kb on + strand at 1230.327 kb on + strand at 1230.327 kb on + strand at 1230.445 kb on + strand, within nrdR at 1230.485 kb on + strand, within nrdR at 1230.485 kb on + strand, within nrdR at 1230.741 kb on + strand, within nrdR at 1230.741 kb on + strand, within nrdR at 1230.741 kb on + strand, within nrdR at 1230.741 kb on + strand, within nrdR at 1230.741 kb on + strand, within nrdR at 1230.760 kb on + strand, within nrdR at 1230.760 kb on + strand, within nrdR at 1230.819 kb on + strand at 1230.819 kb on + strand at 1230.819 kb on + strand at 1230.819 kb on + strand at 1230.819 kb on + strand at 1230.848 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.125 mg/ml remove 1,229,413 - glyA Sama_1014 0.32 +0.3 1,229,432 + glyA Sama_1014 0.33 +0.8 1,229,432 + glyA Sama_1014 0.33 -0.0 1,229,432 + glyA Sama_1014 0.33 -0.3 1,229,432 + glyA Sama_1014 0.33 -0.6 1,229,432 + glyA Sama_1014 0.33 +0.2 1,229,433 - glyA Sama_1014 0.33 -0.8 1,229,433 - glyA Sama_1014 0.33 +0.2 1,229,433 - glyA Sama_1014 0.33 -0.4 1,229,447 + glyA Sama_1014 0.34 -0.3 1,229,447 + glyA Sama_1014 0.34 +0.3 1,229,477 + glyA Sama_1014 0.37 -1.9 1,229,544 - glyA Sama_1014 0.42 +0.4 1,229,600 + glyA Sama_1014 0.47 -0.4 1,229,600 + glyA Sama_1014 0.47 -1.1 1,229,600 + glyA Sama_1014 0.47 +1.9 1,229,600 + glyA Sama_1014 0.47 +1.5 1,229,600 + glyA Sama_1014 0.47 +0.4 1,229,601 - glyA Sama_1014 0.47 -0.1 1,229,601 - glyA Sama_1014 0.47 -0.6 1,229,601 - glyA Sama_1014 0.47 -0.5 1,229,625 + glyA Sama_1014 0.49 +0.1 1,229,625 + glyA Sama_1014 0.49 -0.6 1,229,625 + glyA Sama_1014 0.49 +0.2 1,229,626 - glyA Sama_1014 0.49 -0.5 1,229,649 + glyA Sama_1014 0.51 +3.0 1,229,649 + glyA Sama_1014 0.51 +0.3 1,229,649 + glyA Sama_1014 0.51 +0.1 1,229,651 + glyA Sama_1014 0.51 -0.1 1,229,680 - glyA Sama_1014 0.53 +0.5 1,229,759 + glyA Sama_1014 0.59 -0.1 1,229,759 + glyA Sama_1014 0.59 +0.1 1,229,760 - glyA Sama_1014 0.59 +0.2 1,229,781 + glyA Sama_1014 0.61 +0.3 1,229,873 + glyA Sama_1014 0.68 +0.7 1,229,873 + glyA Sama_1014 0.68 -0.1 1,229,873 + glyA Sama_1014 0.68 +0.2 1,229,873 + glyA Sama_1014 0.68 +0.4 1,229,916 + glyA Sama_1014 0.72 +0.5 1,229,916 + glyA Sama_1014 0.72 -0.2 1,229,916 + glyA Sama_1014 0.72 +0.5 1,229,917 - glyA Sama_1014 0.72 -0.2 1,229,917 - glyA Sama_1014 0.72 -0.6 1,229,917 - glyA Sama_1014 0.72 +1.4 1,229,917 - glyA Sama_1014 0.72 -1.9 1,230,004 + glyA Sama_1014 0.79 -0.4 1,230,049 + glyA Sama_1014 0.82 +0.3 1,230,049 + glyA Sama_1014 0.82 +0.5 1,230,049 + glyA Sama_1014 0.82 +0.2 1,230,049 + glyA Sama_1014 0.82 +1.9 1,230,181 - -0.7 1,230,181 - -0.7 1,230,181 - -0.6 1,230,181 - +1.7 1,230,181 - -1.5 1,230,231 + +0.2 1,230,231 + +0.7 1,230,260 + -0.1 1,230,261 - +0.9 1,230,261 - +0.2 1,230,274 + +0.3 1,230,283 + -0.5 1,230,283 + -0.4 1,230,288 + +0.1 1,230,288 + +0.9 1,230,288 + -1.0 1,230,288 + -0.2 1,230,321 + -0.8 1,230,327 + -1.5 1,230,327 + +0.8 1,230,327 + -1.0 1,230,327 + +0.7 1,230,445 + nrdR Sama_1015 0.16 -1.8 1,230,485 + nrdR Sama_1015 0.25 -0.9 1,230,485 + nrdR Sama_1015 0.25 -0.3 1,230,741 + nrdR Sama_1015 0.82 -1.8 1,230,741 + nrdR Sama_1015 0.82 -1.3 1,230,741 + nrdR Sama_1015 0.82 -0.1 1,230,741 + nrdR Sama_1015 0.82 -1.6 1,230,741 + nrdR Sama_1015 0.82 -1.0 1,230,760 + nrdR Sama_1015 0.86 -1.7 1,230,760 + nrdR Sama_1015 0.86 -0.6 1,230,819 + +0.5 1,230,819 + -0.1 1,230,819 + +0.1 1,230,819 + +1.8 1,230,819 + -0.2 1,230,848 + +1.1
Or see this region's nucleotide sequence