Experiment: Bacitracin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0823 and Sama_0824 are separated by 117 nucleotides Sama_0824 and Sama_0825 are separated by 81 nucleotides Sama_0825 and rpsU are separated by 6 nucleotides rpsU and Sama_0827 are separated by 232 nucleotides
Sama_0823: Sama_0823 - RNA polymerase sigma-70 factor (RefSeq), at 1,006,839 to 1,008,701
_0823
Sama_0824: Sama_0824 - DNA primase (RefSeq), at 1,008,819 to 1,010,549
_0824
Sama_0825: Sama_0825 - hypothetical protein (RefSeq), at 1,010,631 to 1,011,074
_0825
Sama_0826: rpsU - 30S ribosomal protein S21 (RefSeq), at 1,011,081 to 1,011,296
rpsU
Sama_0827: Sama_0827 - O-sialoglycoprotein endopeptidase (RefSeq), at 1,011,529 to 1,012,542
_0827
Position (kb)
1008
1009
1010
1011 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1008.770 kb on - strand at 1008.770 kb on - strand at 1008.770 kb on - strand at 1008.770 kb on - strand at 1008.772 kb on - strand at 1008.820 kb on + strand at 1010.618 kb on - strand at 1010.618 kb on - strand at 1010.713 kb on - strand, within Sama_0825 at 1010.718 kb on - strand, within Sama_0825 at 1010.746 kb on - strand, within Sama_0825 at 1011.429 kb on + strand at 1011.464 kb on + strand at 1011.472 kb on + strand at 1011.472 kb on + strand at 1011.472 kb on + strand at 1011.472 kb on + strand at 1011.472 kb on + strand at 1011.472 kb on + strand at 1011.472 kb on + strand at 1011.472 kb on + strand at 1011.475 kb on + strand at 1011.475 kb on + strand at 1011.475 kb on + strand at 1011.478 kb on + strand at 1011.478 kb on + strand at 1011.483 kb on + strand at 1011.483 kb on + strand at 1011.484 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.125 mg/ml remove 1,008,770 - +0.2 1,008,770 - -1.4 1,008,770 - -2.2 1,008,770 - -2.2 1,008,772 - -0.4 1,008,820 + -0.0 1,010,618 - -1.2 1,010,618 - -2.5 1,010,713 - Sama_0825 0.18 -1.8 1,010,718 - Sama_0825 0.20 -1.6 1,010,746 - Sama_0825 0.26 -1.8 1,011,429 + -0.5 1,011,464 + -0.2 1,011,472 + -1.2 1,011,472 + -0.8 1,011,472 + +0.6 1,011,472 + -0.2 1,011,472 + -0.4 1,011,472 + -1.0 1,011,472 + -0.9 1,011,472 + -0.8 1,011,475 + -0.8 1,011,475 + -0.8 1,011,475 + -0.9 1,011,478 + -0.7 1,011,478 + -0.6 1,011,483 + -2.8 1,011,483 + -0.5 1,011,484 - +1.0
Or see this region's nucleotide sequence