Experiment: Bacitracin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_R0025 and Sama_R0026 are separated by 66 nucleotides Sama_R0026 and Sama_R0027 are separated by 66 nucleotides Sama_R0027 and Sama_R0028 are separated by 45 nucleotides Sama_R0028 and Sama_R0029 are separated by 42 nucleotides Sama_R0029 and Sama_0363 are separated by 129 nucleotides Sama_0363 and Sama_0364 are separated by 41 nucleotides Sama_0364 and Sama_0365 are separated by 61 nucleotides
Sama_R0025: Sama_R0025 - 23S ribosomal RNA (RefSeq), at 438,231 to 441,144
_R0025
Sama_R0026: Sama_R0026 - tRNA-Val (RefSeq), at 441,211 to 441,286
_R0026
Sama_R0027: Sama_R0027 - 5S ribosomal RNA (RefSeq), at 441,353 to 441,468
_R0027
Sama_R0028: Sama_R0028 - tRNA-Thr (RefSeq), at 441,514 to 441,589
_R0028
Sama_R0029: Sama_R0029 - 5S ribosomal RNA (RefSeq), at 441,632 to 441,746
_R0029
Sama_0363: Sama_0363 - mutator MutT protein (RefSeq), at 441,876 to 442,271
_0363
Sama_0364: Sama_0364 - hypothetical protein (RefSeq), at 442,313 to 442,528
_0364
Sama_0365: Sama_0365 - hypothetical protein (RefSeq), at 442,590 to 443,324
_0365
Position (kb) 441
442
443 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 441.287 kb on + strand at 441.287 kb on + strand at 441.287 kb on + strand at 441.287 kb on + strand at 441.287 kb on + strand at 441.287 kb on + strand at 441.287 kb on + strand at 441.287 kb on + strand at 441.453 kb on + strand, within Sama_R0027 at 441.453 kb on + strand, within Sama_R0027 at 441.472 kb on + strand at 441.549 kb on + strand, within Sama_R0028 at 441.549 kb on + strand, within Sama_R0028 at 441.591 kb on + strand at 441.591 kb on + strand at 441.608 kb on + strand at 441.608 kb on + strand at 441.679 kb on + strand, within Sama_R0029 at 441.679 kb on + strand, within Sama_R0029 at 441.704 kb on + strand, within Sama_R0029 at 441.704 kb on + strand, within Sama_R0029 at 441.704 kb on + strand, within Sama_R0029 at 441.730 kb on + strand, within Sama_R0029 at 441.730 kb on + strand, within Sama_R0029 at 441.788 kb on + strand at 441.819 kb on + strand at 441.831 kb on - strand at 441.846 kb on + strand at 441.846 kb on + strand at 441.881 kb on + strand at 441.881 kb on + strand at 441.882 kb on - strand at 441.882 kb on - strand at 441.882 kb on - strand at 441.899 kb on + strand at 441.899 kb on + strand at 441.935 kb on - strand, within Sama_0363 at 441.935 kb on - strand, within Sama_0363 at 442.022 kb on + strand, within Sama_0363 at 442.023 kb on - strand, within Sama_0363 at 442.023 kb on - strand, within Sama_0363 at 442.032 kb on + strand, within Sama_0363 at 442.032 kb on + strand, within Sama_0363 at 442.032 kb on + strand, within Sama_0363 at 442.033 kb on - strand, within Sama_0363 at 442.033 kb on - strand, within Sama_0363 at 442.033 kb on - strand, within Sama_0363 at 442.049 kb on - strand, within Sama_0363 at 442.067 kb on + strand, within Sama_0363 at 442.067 kb on + strand, within Sama_0363 at 442.068 kb on - strand, within Sama_0363 at 442.068 kb on - strand, within Sama_0363 at 442.068 kb on - strand, within Sama_0363 at 442.068 kb on - strand, within Sama_0363 at 442.068 kb on - strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.111 kb on - strand, within Sama_0363 at 442.235 kb on + strand at 442.235 kb on + strand at 442.236 kb on - strand at 442.236 kb on - strand at 442.236 kb on - strand at 442.236 kb on - strand at 442.246 kb on - strand at 442.265 kb on + strand at 442.265 kb on + strand at 442.265 kb on + strand at 442.265 kb on + strand at 442.265 kb on + strand at 442.266 kb on - strand at 442.266 kb on - strand at 442.266 kb on - strand at 442.271 kb on + strand at 442.271 kb on + strand at 442.271 kb on + strand at 442.271 kb on + strand at 442.271 kb on + strand at 442.272 kb on - strand at 442.272 kb on - strand at 442.287 kb on + strand at 442.288 kb on - strand at 442.288 kb on - strand at 442.290 kb on + strand at 442.290 kb on + strand at 442.290 kb on + strand at 442.290 kb on + strand at 442.291 kb on - strand at 442.327 kb on + strand at 442.327 kb on + strand at 442.327 kb on + strand at 442.327 kb on + strand at 442.388 kb on + strand, within Sama_0364 at 442.425 kb on - strand, within Sama_0364 at 442.425 kb on - strand, within Sama_0364 at 442.487 kb on - strand, within Sama_0364 at 442.514 kb on - strand at 442.514 kb on - strand at 442.529 kb on - strand at 442.529 kb on - strand at 442.551 kb on + strand at 442.551 kb on + strand at 442.551 kb on + strand at 442.552 kb on - strand at 442.552 kb on - strand at 442.552 kb on - strand at 442.552 kb on - strand at 442.577 kb on + strand at 442.578 kb on - strand at 442.578 kb on - strand at 442.582 kb on + strand at 442.583 kb on - strand at 442.583 kb on - strand at 442.583 kb on - strand at 442.611 kb on - strand at 442.611 kb on - strand at 442.625 kb on + strand at 442.688 kb on + strand, within Sama_0365 at 442.765 kb on + strand, within Sama_0365 at 442.765 kb on + strand, within Sama_0365 at 442.766 kb on - strand, within Sama_0365 at 442.795 kb on + strand, within Sama_0365 at 442.841 kb on + strand, within Sama_0365 at 442.842 kb on - strand, within Sama_0365 at 442.876 kb on + strand, within Sama_0365 at 442.876 kb on + strand, within Sama_0365 at 442.876 kb on + strand, within Sama_0365 at 442.877 kb on - strand, within Sama_0365 at 442.930 kb on - strand, within Sama_0365 at 442.940 kb on + strand, within Sama_0365 at 442.940 kb on + strand, within Sama_0365 at 442.975 kb on - strand, within Sama_0365 at 442.975 kb on - strand, within Sama_0365 at 442.984 kb on - strand, within Sama_0365 at 442.992 kb on - strand, within Sama_0365 at 443.048 kb on + strand, within Sama_0365 at 443.048 kb on + strand, within Sama_0365 at 443.049 kb on - strand, within Sama_0365 at 443.049 kb on - strand, within Sama_0365 at 443.052 kb on + strand, within Sama_0365 at 443.053 kb on - strand, within Sama_0365 at 443.097 kb on + strand, within Sama_0365 at 443.097 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.100 kb on + strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.101 kb on - strand, within Sama_0365 at 443.121 kb on - strand, within Sama_0365 at 443.121 kb on - strand, within Sama_0365 at 443.136 kb on + strand, within Sama_0365 at 443.162 kb on - strand, within Sama_0365 at 443.178 kb on + strand, within Sama_0365 at 443.178 kb on + strand, within Sama_0365 at 443.188 kb on - strand, within Sama_0365 at 443.196 kb on + strand, within Sama_0365 at 443.196 kb on + strand, within Sama_0365 at 443.196 kb on + strand, within Sama_0365 at 443.197 kb on - strand, within Sama_0365 at 443.237 kb on + strand, within Sama_0365 at 443.237 kb on + strand, within Sama_0365 at 443.237 kb on + strand, within Sama_0365 at 443.237 kb on + strand, within Sama_0365 at 443.238 kb on - strand, within Sama_0365 at 443.238 kb on - strand, within Sama_0365
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.125 mg/ml remove 441,287 + +1.1 441,287 + -2.4 441,287 + -0.8 441,287 + +0.4 441,287 + -0.4 441,287 + -0.5 441,287 + -0.1 441,287 + -1.0 441,453 + Sama_R0027 0.86 -1.4 441,453 + Sama_R0027 0.86 -0.4 441,472 + +0.3 441,549 + Sama_R0028 0.46 -1.8 441,549 + Sama_R0028 0.46 -1.9 441,591 + +0.6 441,591 + -0.4 441,608 + -0.9 441,608 + -0.5 441,679 + Sama_R0029 0.41 -0.0 441,679 + Sama_R0029 0.41 +1.1 441,704 + Sama_R0029 0.63 +0.4 441,704 + Sama_R0029 0.63 -0.2 441,704 + Sama_R0029 0.63 -0.7 441,730 + Sama_R0029 0.85 -0.1 441,730 + Sama_R0029 0.85 -0.2 441,788 + +0.3 441,819 + +1.0 441,831 - +0.7 441,846 + -0.2 441,846 + -0.3 441,881 + +0.9 441,881 + -0.8 441,882 - +0.8 441,882 - -0.2 441,882 - -0.6 441,899 + -0.2 441,899 + -0.2 441,935 - Sama_0363 0.15 -1.8 441,935 - Sama_0363 0.15 +0.5 442,022 + Sama_0363 0.37 +0.2 442,023 - Sama_0363 0.37 +0.2 442,023 - Sama_0363 0.37 -1.0 442,032 + Sama_0363 0.39 -0.0 442,032 + Sama_0363 0.39 -0.1 442,032 + Sama_0363 0.39 +0.5 442,033 - Sama_0363 0.40 -0.7 442,033 - Sama_0363 0.40 -2.9 442,033 - Sama_0363 0.40 +2.1 442,049 - Sama_0363 0.44 +0.4 442,067 + Sama_0363 0.48 +0.7 442,067 + Sama_0363 0.48 -0.8 442,068 - Sama_0363 0.48 -0.9 442,068 - Sama_0363 0.48 +0.2 442,068 - Sama_0363 0.48 +0.9 442,068 - Sama_0363 0.48 +0.4 442,068 - Sama_0363 0.48 +0.2 442,069 + Sama_0363 0.49 +0.1 442,069 + Sama_0363 0.49 +0.5 442,069 + Sama_0363 0.49 +0.3 442,069 + Sama_0363 0.49 +0.7 442,069 + Sama_0363 0.49 +0.6 442,069 + Sama_0363 0.49 +0.1 442,069 + Sama_0363 0.49 +0.2 442,070 - Sama_0363 0.49 -0.2 442,070 - Sama_0363 0.49 +0.4 442,070 - Sama_0363 0.49 -0.3 442,070 - Sama_0363 0.49 -0.3 442,070 - Sama_0363 0.49 -0.4 442,070 - Sama_0363 0.49 +0.8 442,070 - Sama_0363 0.49 +0.5 442,070 - Sama_0363 0.49 +0.6 442,070 - Sama_0363 0.49 -0.3 442,070 - Sama_0363 0.49 +0.1 442,070 - Sama_0363 0.49 +0.3 442,070 - Sama_0363 0.49 -0.4 442,070 - Sama_0363 0.49 +0.4 442,111 - Sama_0363 0.59 +0.3 442,235 + -0.7 442,235 + +0.1 442,236 - +0.5 442,236 - -0.4 442,236 - +0.6 442,236 - -0.2 442,246 - -0.8 442,265 + -0.3 442,265 + -0.5 442,265 + -0.0 442,265 + -0.4 442,265 + +0.9 442,266 - +0.9 442,266 - -2.1 442,266 - -0.7 442,271 + -1.1 442,271 + -0.6 442,271 + +0.2 442,271 + -0.1 442,271 + -0.9 442,272 - -0.1 442,272 - -2.0 442,287 + +0.4 442,288 - -0.1 442,288 - -1.9 442,290 + +0.8 442,290 + +1.1 442,290 + +0.0 442,290 + +0.4 442,291 - -0.2 442,327 + +0.6 442,327 + -0.6 442,327 + -0.9 442,327 + -0.8 442,388 + Sama_0364 0.35 +0.4 442,425 - Sama_0364 0.52 -1.2 442,425 - Sama_0364 0.52 -0.4 442,487 - Sama_0364 0.81 -1.6 442,514 - -2.4 442,514 - -0.1 442,529 - +0.5 442,529 - -0.7 442,551 + +0.2 442,551 + -0.7 442,551 + -0.4 442,552 - +0.7 442,552 - +0.0 442,552 - +0.5 442,552 - -0.8 442,577 + -0.7 442,578 - -0.8 442,578 - -0.6 442,582 + -0.0 442,583 - -0.6 442,583 - +0.4 442,583 - -0.5 442,611 - -0.6 442,611 - +0.1 442,625 + -0.0 442,688 + Sama_0365 0.13 -0.2 442,765 + Sama_0365 0.24 +0.3 442,765 + Sama_0365 0.24 +1.7 442,766 - Sama_0365 0.24 -1.4 442,795 + Sama_0365 0.28 -1.6 442,841 + Sama_0365 0.34 +0.3 442,842 - Sama_0365 0.34 +0.2 442,876 + Sama_0365 0.39 +0.9 442,876 + Sama_0365 0.39 -0.4 442,876 + Sama_0365 0.39 +1.3 442,877 - Sama_0365 0.39 +0.1 442,930 - Sama_0365 0.46 +0.7 442,940 + Sama_0365 0.48 -0.1 442,940 + Sama_0365 0.48 -0.2 442,975 - Sama_0365 0.52 -0.4 442,975 - Sama_0365 0.52 -0.5 442,984 - Sama_0365 0.54 -1.0 442,992 - Sama_0365 0.55 -0.7 443,048 + Sama_0365 0.62 +0.4 443,048 + Sama_0365 0.62 +0.9 443,049 - Sama_0365 0.62 +0.5 443,049 - Sama_0365 0.62 +0.7 443,052 + Sama_0365 0.63 +1.1 443,053 - Sama_0365 0.63 +0.1 443,097 + Sama_0365 0.69 +0.4 443,097 + Sama_0365 0.69 +0.7 443,100 + Sama_0365 0.69 +0.3 443,100 + Sama_0365 0.69 +0.1 443,100 + Sama_0365 0.69 +0.6 443,100 + Sama_0365 0.69 -1.5 443,100 + Sama_0365 0.69 +0.4 443,100 + Sama_0365 0.69 -0.4 443,100 + Sama_0365 0.69 +0.1 443,100 + Sama_0365 0.69 -0.1 443,100 + Sama_0365 0.69 -0.5 443,101 - Sama_0365 0.70 +0.6 443,101 - Sama_0365 0.70 +0.2 443,101 - Sama_0365 0.70 +0.4 443,101 - Sama_0365 0.70 -0.3 443,101 - Sama_0365 0.70 +0.1 443,101 - Sama_0365 0.70 +0.2 443,101 - Sama_0365 0.70 -0.1 443,101 - Sama_0365 0.70 +0.3 443,101 - Sama_0365 0.70 -0.8 443,101 - Sama_0365 0.70 +0.8 443,121 - Sama_0365 0.72 +0.6 443,121 - Sama_0365 0.72 -0.9 443,136 + Sama_0365 0.74 +0.5 443,162 - Sama_0365 0.78 -0.2 443,178 + Sama_0365 0.80 +0.6 443,178 + Sama_0365 0.80 -0.0 443,188 - Sama_0365 0.81 +0.9 443,196 + Sama_0365 0.82 +0.4 443,196 + Sama_0365 0.82 +0.1 443,196 + Sama_0365 0.82 +0.0 443,197 - Sama_0365 0.83 +0.2 443,237 + Sama_0365 0.88 +0.8 443,237 + Sama_0365 0.88 +0.9 443,237 + Sama_0365 0.88 +0.3 443,237 + Sama_0365 0.88 +0.2 443,238 - Sama_0365 0.88 +2.9 443,238 - Sama_0365 0.88 +0.6
Or see this region's nucleotide sequence