Strain Fitness in Shewanella oneidensis MR-1 around SO0603

Experiment: NAG (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmiaA and hfq are separated by 91 nucleotideshfq and hflX are separated by 23 nucleotides SO0602: miaA - tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI ptt file), at 628,738 to 629,664 miaA SO0603: hfq - host factor-I protein (NCBI ptt file), at 629,756 to 630,028 hfq SO0604: hflX - GTP-binding protein HflX (NCBI ptt file), at 630,052 to 631,359 hflX Position (kb) 629 630 631Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 628.891 kb on + strand, within miaAat 629.010 kb on + strand, within miaAat 629.302 kb on + strand, within miaAat 629.395 kb on + strand, within miaAat 629.471 kb on + strand, within miaAat 629.942 kb on + strand, within hfqat 629.999 kb on + strand, within hfqat 630.310 kb on + strand, within hflXat 630.354 kb on + strand, within hflXat 630.359 kb on + strand, within hflXat 630.402 kb on - strand, within hflXat 630.502 kb on + strand, within hflXat 630.697 kb on + strand, within hflXat 630.697 kb on + strand, within hflXat 630.697 kb on + strand, within hflXat 630.774 kb on + strand, within hflXat 630.803 kb on + strand, within hflX

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Per-strain Table

Position Strand Gene LocusTag Fraction NAG (C)
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628,891 + miaA SO0602 0.17 -2.4
629,010 + miaA SO0602 0.29 -3.2
629,302 + miaA SO0602 0.61 -2.2
629,395 + miaA SO0602 0.71 -2.2
629,471 + miaA SO0602 0.79 -3.0
629,942 + hfq SO0603 0.68 -5.0
629,999 + hfq SO0603 0.89 -2.6
630,310 + hflX SO0604 0.20 -2.1
630,354 + hflX SO0604 0.23 -5.0
630,359 + hflX SO0604 0.23 -4.6
630,402 - hflX SO0604 0.27 +0.2
630,502 + hflX SO0604 0.34 -2.4
630,697 + hflX SO0604 0.49 -3.7
630,697 + hflX SO0604 0.49 -3.7
630,697 + hflX SO0604 0.49 -3.3
630,774 + hflX SO0604 0.55 -3.9
630,803 + hflX SO0604 0.57 -3.5

Or see this region's nucleotide sequence