Experiment: m.b. 1-ethyl-3-methylimidazolium chloride 7.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_250 and HP15_251 are separated by 68 nucleotides HP15_251 and HP15_252 overlap by 7 nucleotides HP15_252 and HP15_253 are separated by 183 nucleotides
HP15_250: HP15_250 - site-specific tyrosine recombinase XerC, at 238,530 to 239,462
_250
HP15_251: HP15_251 - diguanylate cyclase, at 239,531 to 241,024
_251
HP15_252: HP15_252 - conserved hypothetical protein, at 241,018 to 241,257
_252
HP15_253: HP15_253 - extracellular solute-binding protein, family 3, at 241,441 to 244,149
_253
Position (kb) 239
240
241
242 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 238.929 kb on - strand, within HP15_250 at 239.026 kb on - strand, within HP15_250 at 239.031 kb on - strand, within HP15_250 at 239.031 kb on - strand, within HP15_250 at 239.102 kb on + strand, within HP15_250 at 239.112 kb on - strand, within HP15_250 at 239.193 kb on + strand, within HP15_250 at 239.330 kb on - strand, within HP15_250 at 239.330 kb on - strand, within HP15_250 at 239.354 kb on + strand, within HP15_250 at 239.362 kb on - strand, within HP15_250 at 239.548 kb on - strand at 239.605 kb on - strand at 239.630 kb on - strand at 239.663 kb on + strand at 239.844 kb on + strand, within HP15_251 at 239.865 kb on - strand, within HP15_251 at 239.878 kb on + strand, within HP15_251 at 239.883 kb on + strand, within HP15_251 at 239.922 kb on - strand, within HP15_251 at 240.021 kb on + strand, within HP15_251 at 240.021 kb on + strand, within HP15_251 at 240.021 kb on + strand, within HP15_251 at 240.029 kb on - strand, within HP15_251 at 240.029 kb on - strand, within HP15_251 at 240.104 kb on + strand, within HP15_251 at 240.104 kb on + strand, within HP15_251 at 240.104 kb on + strand, within HP15_251 at 240.104 kb on + strand, within HP15_251 at 240.104 kb on + strand, within HP15_251 at 240.112 kb on + strand, within HP15_251 at 240.112 kb on - strand, within HP15_251 at 240.112 kb on - strand, within HP15_251 at 240.125 kb on - strand, within HP15_251 at 240.135 kb on - strand, within HP15_251 at 240.206 kb on + strand, within HP15_251 at 240.214 kb on + strand, within HP15_251 at 240.233 kb on + strand, within HP15_251 at 240.236 kb on - strand, within HP15_251 at 240.236 kb on - strand, within HP15_251 at 240.246 kb on - strand, within HP15_251 at 240.263 kb on - strand, within HP15_251 at 240.327 kb on + strand, within HP15_251 at 240.329 kb on - strand, within HP15_251 at 240.357 kb on - strand, within HP15_251 at 240.436 kb on + strand, within HP15_251 at 240.444 kb on - strand, within HP15_251 at 240.444 kb on - strand, within HP15_251 at 240.451 kb on + strand, within HP15_251 at 240.472 kb on + strand, within HP15_251 at 240.485 kb on - strand, within HP15_251 at 240.489 kb on + strand, within HP15_251 at 240.509 kb on + strand, within HP15_251 at 240.544 kb on - strand, within HP15_251 at 240.553 kb on - strand, within HP15_251 at 240.559 kb on + strand, within HP15_251 at 240.610 kb on - strand, within HP15_251 at 240.629 kb on + strand, within HP15_251 at 240.642 kb on - strand, within HP15_251 at 240.672 kb on - strand, within HP15_251 at 240.921 kb on + strand at 240.967 kb on + strand at 240.981 kb on + strand at 240.991 kb on + strand at 241.000 kb on - strand at 241.016 kb on - strand at 241.120 kb on - strand, within HP15_252 at 241.120 kb on - strand, within HP15_252 at 241.333 kb on + strand at 241.392 kb on + strand at 241.454 kb on - strand at 241.559 kb on + strand at 241.630 kb on - strand at 241.644 kb on + strand at 241.672 kb on + strand at 241.680 kb on - strand at 241.695 kb on - strand at 241.877 kb on - strand, within HP15_253
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 1-ethyl-3-methylimidazolium chloride 7.5 mM remove 238,929 - HP15_250 0.43 -2.5 239,026 - HP15_250 0.53 -2.6 239,031 - HP15_250 0.54 -2.9 239,031 - HP15_250 0.54 -3.0 239,102 + HP15_250 0.61 -0.4 239,112 - HP15_250 0.62 -2.6 239,193 + HP15_250 0.71 -1.4 239,330 - HP15_250 0.86 -0.7 239,330 - HP15_250 0.86 -1.4 239,354 + HP15_250 0.88 -2.1 239,362 - HP15_250 0.89 -1.3 239,548 - -0.3 239,605 - +0.5 239,630 - -0.4 239,663 + +1.1 239,844 + HP15_251 0.21 +0.7 239,865 - HP15_251 0.22 -0.1 239,878 + HP15_251 0.23 +0.9 239,883 + HP15_251 0.24 -0.9 239,922 - HP15_251 0.26 +0.1 240,021 + HP15_251 0.33 -0.1 240,021 + HP15_251 0.33 -0.9 240,021 + HP15_251 0.33 -0.3 240,029 - HP15_251 0.33 +0.7 240,029 - HP15_251 0.33 +0.9 240,104 + HP15_251 0.38 +0.7 240,104 + HP15_251 0.38 -0.1 240,104 + HP15_251 0.38 -0.6 240,104 + HP15_251 0.38 -0.4 240,104 + HP15_251 0.38 +0.4 240,112 + HP15_251 0.39 +0.3 240,112 - HP15_251 0.39 -1.5 240,112 - HP15_251 0.39 -1.3 240,125 - HP15_251 0.40 -0.1 240,135 - HP15_251 0.40 +0.4 240,206 + HP15_251 0.45 +0.4 240,214 + HP15_251 0.46 -0.0 240,233 + HP15_251 0.47 -1.7 240,236 - HP15_251 0.47 +1.9 240,236 - HP15_251 0.47 -0.5 240,246 - HP15_251 0.48 +1.0 240,263 - HP15_251 0.49 +0.3 240,327 + HP15_251 0.53 +0.4 240,329 - HP15_251 0.53 +1.4 240,357 - HP15_251 0.55 +0.0 240,436 + HP15_251 0.61 +0.6 240,444 - HP15_251 0.61 +0.2 240,444 - HP15_251 0.61 +0.4 240,451 + HP15_251 0.62 +0.2 240,472 + HP15_251 0.63 +0.1 240,485 - HP15_251 0.64 +0.2 240,489 + HP15_251 0.64 -0.5 240,509 + HP15_251 0.65 +0.1 240,544 - HP15_251 0.68 -0.7 240,553 - HP15_251 0.68 -1.8 240,559 + HP15_251 0.69 +2.6 240,610 - HP15_251 0.72 -0.5 240,629 + HP15_251 0.73 +0.3 240,642 - HP15_251 0.74 -0.2 240,672 - HP15_251 0.76 +0.7 240,921 + +2.2 240,967 + -0.0 240,981 + +0.1 240,991 + -0.3 241,000 - -0.6 241,016 - -0.4 241,120 - HP15_252 0.42 +0.1 241,120 - HP15_252 0.42 -0.9 241,333 + -1.0 241,392 + -2.2 241,454 - -0.1 241,559 + -1.9 241,630 - -0.1 241,644 + -1.9 241,672 + -0.4 241,680 - -2.4 241,695 - -2.2 241,877 - HP15_253 0.16 -2.6
Or see this region's nucleotide sequence